scholarly journals Protein arginine methyltransferase 5 (PRMT5) activates WNT/β‐catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3

2021 ◽  
Vol 25 (3) ◽  
pp. 1583-1600
Author(s):  
Harshita Shailesh ◽  
Kodappully S. Siveen ◽  
Saïd Sif
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Epigenetic Silencing ◽  
Protein Arginine Methyltransferase ◽  
Arginine Methyltransferase ◽  
Protein Arginine Methyltransferase 5

scholarly journals Protein arginine methyltransferase 6-dependent gene expression and splicing: association with breast cancer outcomes

2012 ◽  
Vol 19 (4) ◽  
pp. 509-526 ◽  
Author(s):  
Dennis H Dowhan ◽  
Matthew J Harrison ◽  
Natalie A Eriksson ◽  
Peter Bailey ◽  
Michael A Pearen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Alternative Splicing ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Gene Expression Signature ◽  
Human Breast ◽  
Arginine Methyltransferase ◽  
Breast Tumours

Protein arginine methyltransferase-6 (PRMT6) regulates steroid-dependent transcription and alternative splicing and is implicated in endocrine system development and function, cell death, cell cycle, gene expression and cancer. Despite its role in these processes, little is known about its function and cellular targets in breast cancer. To identify novel gene targets regulated by PRMT6 in breast cancer cells, we used a combination of small interfering RNA and exon-specific microarray profilingin vitrocoupled toin vivovalidation in normal breast and primary human breast tumours. This approach, which allows the examination of genome-wide changes in individual exon usage and total transcript levels, demonstrated thatPRMT6knockdown significantly affected i) the transcription of 159 genes and ii) alternate splicing of 449 genes. ThePRMT6-dependent transcriptional and alternative splicing targets identifiedin vitrowere validated in human breast tumours. Using the list of genes differentially expressed between normal andPRMT6knockdown cells, we generated aPRMT6-dependent gene expression signature that provides an indication of PRMT6 dysfunction in breast cancer cells. Interrogation of several well-studied breast cancer microarray expression datasets with thePRMT6gene expression signature demonstrated that PRMT6 dysfunction is associated with better overall relapse-free and distant metastasis-free survival in the oestrogen receptor (ER (ESR1)) breast cancer subgroup. These results suggest that dysregulation ofPRMT6-dependent transcription and alternative splicing may be involved in breast cancer pathophysiology and the molecular consequences identifying a unique and informative biomarker profile.

scholarly journals Resveratrol Prevents Epigenetic Silencing of BRCA-1 by the Aromatic Hydrocarbon Receptor in Human Breast Cancer Cells

2010 ◽  
Vol 140 (9) ◽  
pp. 1607-1614 ◽  
Author(s):  
Andreas J. Papoutsis ◽  
Sarah D. Lamore ◽  
Georg T. Wondrak ◽  
Ornella I. Selmin ◽  
Donato F. Romagnolo
Keyword(s):  
Breast Cancer ◽  
Aromatic Hydrocarbon ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Epigenetic Silencing ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Aromatic Hydrocarbon Receptor ◽  
Brca 1

scholarly journals Epigenetic silencing of RNF144A expression in breast cancer cells through promoter hypermethylation and MBD4

2018 ◽  
Vol 7 (4) ◽  
pp. 1317-1325 ◽  
Author(s):  
Ye Zhang ◽  
Yin-Long Yang ◽  
Fang-Lin Zhang ◽  
Xiao-Hong Liao ◽  
Zhi-Min Shao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Promoter Hypermethylation ◽  
Epigenetic Silencing

scholarly journals Protein arginine methyltransferase 5 is essential for growth of lung cancer cells

2012 ◽  
Vol 446 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Zhongping Gu ◽  
Shen Gao ◽  
Fahao Zhang ◽  
Zhiqiang Wang ◽  
Wencai Ma ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
A549 Cells ◽  
Lung Cancer Cells ◽  
In Vivo Studies ◽  
Human Lung Tissue ◽  
Protein Arginine Methyltransferase ◽  
Arginine Methyltransferase ◽  
Arginine Residues ◽  
Protein Arginine Methyltransferase 5

PRMT5 (protein arginine methyltransferase 5) is an enzyme that catalyses transfer of methyl groups from S-adenosyl methionine to the arginine residues of histones or non-histone proteins and is involved in a variety of cellular processes. Although it is highly expressed in some tumours, its direct role in cancer growth has not been fully investigated. In the present study, in human lung tissue samples we found that PRMT5 was highly expressed in lung cancer cells, whereas its expression was not detectable in benign lung tissues. Silencing PRMT5 expression strongly inhibited proliferation of lung adenocarcinoma A549 cells in tissue culture, and silencing PRMT5 expression in A549 cells also abolished growth of lung A549 xenografts in mice. In vitro and in vivo studies showed that the cell growth arrest induced by loss of PRMT5 expression was partially attributable to down-regulation of fibroblast growth factor receptor signalling. These results suggest that PRMT5 and its methyltransferase activity is essential for proliferation of lung cancer cells and may serve as a novel target for the treatment of lung cancer.

scholarly journals Epigenetic Silencing of miR-203 Upregulates SNAI2 and Contributes to the Invasiveness of Malignant Breast Cancer Cells

2011 ◽  
Vol 2 (8) ◽  
pp. 782-791 ◽  
Author(s):  
Z. Zhang ◽  
B. Zhang ◽  
W. Li ◽  
L. Fu ◽  
L. Fu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Epigenetic Silencing ◽  
Malignant Breast
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