scholarly journals High‐mobility group box‐1 promotes vascular calcification in diabetic mice via endoplasmic reticulum stress

2021 ◽  
Author(s):  
Zheng Chen ◽  
Ran Li ◽  
Li‐Gang Pei ◽  
Zhong‐Hai Wei ◽  
Jun Xie ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Vascular Calcification ◽  
High Mobility ◽  
High Mobility Group ◽  
Diabetic Mice

scholarly journals High-mobility group box 1 induces endoplasmic reticulum stress and activates hepatic stellate cells

2018 ◽  
Vol 98 (9) ◽  
pp. 1200-1210 ◽  
Author(s):  
Qin He ◽  
Yu Fu ◽  
Xiangming Ding ◽  
Dongxiao Li ◽  
Zi Wang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Hepatic Stellate Cells ◽  
High Mobility ◽  
Stellate Cells ◽  
High Mobility Group

scholarly journals High Mobility Group B-1 (HMGB-1) Promotes Apoptosis of Macrophage-Derived Foam Cells by Inducing Endoplasmic Reticulum Stress

2018 ◽  
Vol 48 (3) ◽  
pp. 1019-1029 ◽  
Author(s):  
Han Wu ◽  
Zheng Chen ◽  
Jian-Zhou Chen ◽  
Li-Gang Pei ◽  
Jun Xie ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Lipid Accumulation ◽  
High Mobility ◽  
Foam Cells ◽  
High Mobility Group ◽  
Foam Cell Formation ◽  
Dependent Manner ◽  
Group B

Background/Aims: High mobility group B-1 (HMGB-1)-induced endoplasmic reticulum stress (ERS) has been implicated in inflammation and dendritic cell maturation. C/EBP-homologous protein (CHOP) is a vital component of ERS and apoptosis and plays a critical role in atherosclerosis. However, only a little information is available about the role of HMGB-1 in foam cell formation. Thus, the role of HMGB-1-induced ERS/CHOP pathway in apoptosis and formation of macrophage-derived foam cells is investigated. Methods: RAW264.7 cells were treated with oxidized low-density lipoprotein (oxLDL) in the absence and/or presence of HMGB-1, N-acetylcysteine (NAC, an antioxidant), glycyrrhizin (Gly, an HMGB-1 inhibitor), tunicamycin (TM, an ERS inducer), and 4-phenylbutyrate (4-PBA, an ERS inhibitor). Reactive oxygen species (ROS) production was examined by dihydroethidium (DHE) staining. Oil Red O staining, intracellular total cholesterol assay, and Dil-oxLDL uptake assay evaluated the accumulation of lipids in macrophages. Cell apoptosis was measured by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Western blot detected the expression of HMGB-1/ERS/CHOP pathway. Results: oxLDL induced HMGB-1 translocation and secretion in a dose- and time-dependent manner, which was inhibited by NAC. oxLDL-induced lipid accumulation in macrophages was promoted synergistically by HMGB-1 that was attenuated by Gly. Moreover, TM synergized with oxLDL induced lipid accumulation and apoptosis of macrophages; however, 4-PBA alleviated the oxLDL-induced apoptotic foam cells. Additionally, the inhibition of ERS with 4-PBA suppressed the expression of HMGB-1-induced CHOP. Conclusions: OxLDL triggered HMGB-1 secretion in macrophages via oxidative stress. Furthermore, HMGB-1 promoted the formation and apoptosis of macrophage-derived foam cells via activation of ERS/CHOP pathway.

scholarly journals High-Mobility Group Box-1 Protein Promotes Angiogenesis After Peripheral Ischemia in Diabetic Mice Through a VEGF-Dependent Mechanism

Diabetes ◽  
2010 ◽  
Vol 59 (6) ◽  
pp. 1496-1505 ◽  
Author(s):  
F. Biscetti ◽  
G. Straface ◽  
R. De Cristofaro ◽  
S. Lancellotti ◽  
P. Rizzo ◽  
...  
Keyword(s):  
High Mobility ◽  
High Mobility Group ◽  
Diabetic Mice ◽  
Peripheral Ischemia ◽  
Dependent Mechanism

25-Hydroxycholesterol promotes vascular calcification via activation of endoplasmic reticulum stress

2020 ◽  
Vol 880 ◽  
pp. 173165 ◽  
Author(s):  
Qianqian Dong ◽  
Yanting Chen ◽  
Wantao Liu ◽  
Xiaoyu Liu ◽  
An Chen ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Vascular Calcification
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