scholarly journals microRNA‐106b derived from endothelial cell–secreted extracellular vesicles prevents skin wound healing by inhibiting JMJD3 and RIPK3

2021 ◽  
Author(s):  
Lin Qi ◽  
Yufeng Lu ◽  
Zhaolin Wang ◽  
Guiyun Zhang
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Extracellular Vesicles ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Andrea da Fonseca Ferreira ◽  
Pricila da Silva Cunha ◽  
Virgínia Mendes Carregal ◽  
Priscila de Cássia da Silva ◽  
Marcelo Coutinho de Miranda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Average Diameter ◽  
Skin Wound ◽  
Akt Pathway ◽  
Skin Wound Healing ◽  
Akt Phosphorylation ◽  
Akt Activation

Mesenchymal stem/stromal cells (MSCs) are promising tools in cell therapy. They secrete extracellular vesicles (EVs) that carry different classes of molecules that can promote skin repair, but the mechanisms are poorly understood. Skin wound healing is a complex process that requires the activity of several signaling pathways and cell types, including keratinocytes and fibroblasts. In this study, we explored whether adipose tissue MSC-derived EVs could accelerate migration and proliferation of keratinocytes and fibroblasts, activate the AKT pathway, and promote wound healing in vivo. Furthermore, we evaluated if EV effects are miR-205 dependent. We found that MSC EVs had an average diameter of 135 nm. Keratinocytes and fibroblasts exposed to EVs exhibited higher levels of proliferation, migration, and AKT activation. Topical administration of EVs accelerated skin wound closure. Knockdown of miR-205 decreased AKT phosphorylation in fibroblasts and keratinocytes, whereas migration was decreased only in keratinocytes. Moreover, knockdown of miR-205 failed to inhibit AKT phosphorylation in fibroblasts and keratinocytes exposed to EVs. About the mechanism of EV effects, we found that incubation with EVs prevented inhibition of AKT activation by miR-205 knockdown, suggesting that EVs activate AKT independently of miR-205. In conclusion, we demonstrated that EVs are a promising tool for wound healing.

Bone marrow-derived mesenchymal stem cells and extracellular vesicles enriched collagen chitosan scaffold in skin wound healing (a rat model)

2020 ◽  
pp. 088532822096392
Author(s):  
Salma Abolgheit ◽  
Sally Abdelkader ◽  
Moustafa Aboushelib ◽  
Enas Omar ◽  
Radwa Mehanna
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Tensile Strength ◽  
Mesenchymal Stem Cells ◽  
Extracellular Vesicles ◽  
Skin Wound ◽  
Collagen Deposition ◽  
Skin Wound Healing ◽  
Chitosan Scaffolds

Background Over the past ten years, regenerative medicine has focused on the regeneration and the reconstruction of damaged, diseased, or lost tissues and organs. Skin, being the largest organ in the human body, had attained a good attraction in this field. Delayed wound healing is one of the most challenging clinical medicine complications. This study aimed to evaluate the collagen chitosan scaffold’s effect alone, or enriched with either bone marrow-derived mesenchymal stem cells (BM-MSCs) or their secreted extracellular vesicles (EVs) on the duration and quality of skin wound healing. Methods A full-thickness skin wound was induced on the back of 32 adult male Sprague-Dawley rats. The wounds were either covered with collagen chitosan scaffolds alone, scaffolds enriched with stem cells, or extracellular vesicles. Unprotected wounds were used as control. Healing duration, collagen deposition and alignment, CD 68+ macrophage count, and functional tensile strength of healed skin were assessed (α = 0.05, n = 8). Results The rate of skin healing was significantly accelerated in all treated groups compared to the control. Immuno-histochemical assessment of CD68+ macrophages showed enhanced macrophages count, in addition to higher collagen deposition and better collagen alignment in EVs and BM-MSCs treated groups compared to the control group. Higher tensile strength values reflected the better collagen deposition and alignment for these groups. EVs showed higher amounts of collagen deposition and better alignment compared to MSCs treated group. Conclusion The collagen chitosan scaffolds enriched with MSCs or their EVs improved wound healing and improved the quantity and remodeling of collagen with a better assignment to EVs.

scholarly journals Extracellular Vesicles in Skin Wound Healing

2021 ◽  
Author(s):  
Deimantė Narauskaitė ◽  
Gabrielė Vydmantaitė ◽  
Justina Rusteikaitė ◽  
Revathi Sampath ◽  
Akvilė Rudaitytė ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Current Knowledge ◽  
Skin Wound ◽  
Surface Proteins ◽  
Therapeutic Interventions ◽  
Skin Wound Healing ◽  
Skin Repair ◽  
Healing Wound ◽  
Healing Phase

Each year, millions of individuals suffer from a non-healing wound, abnormal scarring, or injuries accompanied by an infection. For these cases, scientists are searching for new therapeutic interventions, from which one of the most promising is the use of extracellular vesicles (EVs). Naturally, EV-based signalling takes part in all four wound healing phases: hemostasis, inflammation, proliferation and remodelling. Such an extensive involvement of EVs suggests exploiting their action to modulate the impaired healing phase. Furthermore, next to their natural wound healing capacity, EVs can be engineered for better defined pharmaceutical purposes, such as carrying specific cargo or targeting specific destinations by labelling them with certain surface proteins. This review aims to promote scientific awareness in basic and translational research of EVs by summarizing the current knowledge about their natural role in each stage of skin repair and the most recent findings in application areas, such as wound healing, skin regeneration and treatment of dermal diseases, including the stem cell-derived, plant-derived and engineered EVs.

scholarly journals Extracellular Vesicles in Skin Wound Healing

2021 ◽  
Vol 14 (8) ◽  
pp. 811
Author(s):  
Deimantė Narauskaitė ◽  
Gabrielė Vydmantaitė ◽  
Justina Rusteikaitė ◽  
Revathi Sampath ◽  
Akvilė Rudaitytė ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Current Knowledge ◽  
Skin Wound ◽  
Surface Proteins ◽  
Therapeutic Interventions ◽  
Skin Wound Healing ◽  
Skin Repair ◽  
Healing Wound ◽  
Healing Phase

Each year, millions of individuals suffer from a non-healing wound, abnormal scarring, or injuries accompanied by an infection. For these cases, scientists are searching for new therapeutic interventions, from which one of the most promising is the use of extracellular vesicles (EVs). Naturally, EV-based signaling takes part in all four wound healing phases: hemostasis, inflammation, proliferation, and remodeling. Such an extensive involvement of EVs suggests exploiting their action to modulate the impaired healing phase. Furthermore, next to their natural wound healing capacity, EVs can be engineered for better defined pharmaceutical purposes, such as carrying specific cargo or targeting specific destinations by labelling them with certain surface proteins. This review aims to promote scientific awareness in basic and translational research of EVs by summarizing the current knowledge about their natural role in each stage of skin repair and the most recent findings in application areas, such as wound healing, skin regeneration, and treatment of dermal diseases, including the stem cell-derived, plant-derived, and engineered EVs.

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