scholarly journals IGF2BP3 facilitates cell proliferation and tumorigenesis via modulation of JAK/STAT signalling pathway in human bladder cancer

2020 ◽  
Vol 24 (23) ◽  
pp. 13949-13960
Author(s):  
Wei Huang ◽  
Yuanyuan Li ◽  
Cheng Zhang ◽  
Huihai Zha ◽  
Xiaocheng Zhou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Signalling Pathway ◽  
Human Bladder Cancer ◽  
Human Bladder

scholarly journals LncRNA HAND2-AS1 exerts anti-oncogenic effects on bladder cancer via restoration of RARB as a sponge of microRNA-146

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Liping Shan ◽  
Wei Liu ◽  
Yunhong Zhan
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Noncoding Rna ◽  
Caspase 3 ◽  
Retinoic Acid Receptor ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Link Type ◽  
Retinoic Acid Receptor Beta ◽  
Receptor Beta

Abstract Background Growing evidence has shown that long noncoding RNA: microRNA: mRNA is implicated in tumor initiation, development, and progression. Long noncoding RNA HAND2-AS1 exhibits anti-cancer effects in diverse cancers. However, the knowledge of HAND-AS1 in bladder cancer development remains unknown. Methods LncRNA and miRNA microarray was conducted to explore different expressed RNA in primary bladder cancer specimens. RNA-RNA interaction prediction tools miRcode (http://www.mircode.org/), DIANA-lncBase v2 (https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2%2Findex-experimental), DIANA-TarBase v.8 (https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=tarbasev8%2Findex) and miRDB (http://www.mirdb.org/) were employed to predict the interactions between RNA. Bladder cancer cell lines were used to perform cell proliferation and apoptosis assays. Western blot and quantitative Real-time Polymerase Chain Reaction were used to determine the expression of protein and RNA separately. Dual-luciferase assay was conducted to determine the activity of three prime untranslated region of retinoic acid receptor beta (RARB). Furthermore, 5637 human bladder cancer mouse models were established to investigate the interactions of lncRNA: miRNA: mRNA in vivo. Results Based on the RT2 lncRNA PCR Arrays analysis, we validated HAND2-AS1 declined in bladder cancer and negatively correlated with the depth of invasion and grades. The overexpression of HAND2-AS1 in human bladder cancer cells 5637 and RT4 hampered cell proliferation by provoking Caspase 3-triggered cell apoptosis. Besides, one of the HAND2-AS1 sponges, miR-146, elevated in bladder cancer and targeted the tumor suppressor, retinoic acid receptor beta (RARB). We further demonstrated that the HAND2-AS1: miR-146: RARB complex promoted Caspase 3-mediated apoptosis by suppressing COX-2 expression. Finally, the results gained in mouse xenografts suggested that HAND2-AS1 diminished miR-146 expression, thereby reversing the suppression of miR-146 on RARB-mediated apoptosis and contributing to bladder cancer regression. Conclusion The present study sheds light on the fact that lncRNA HAND2-AS1 exerted as a tumor suppressor by releasing RARB from miR-146, leading to tumor proliferation and invasion inhibition. The findings expanded HAND2-AS-mediated regulatory networks' knowledge and provided novel insights to improve the RARB-targeted regimens against bladder cancer.

Rab23 is overexpressed in human bladder cancer and promotes cancer cell proliferation and invasion

Tumor Biology ◽  
2015 ◽  
Vol 37 (6) ◽  
pp. 8131-8138 ◽  
Author(s):  
Yuanjun Jiang ◽  
Yushuang Han ◽  
Chaonan Sun ◽  
Chuyang Han ◽  
Ning Han ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Proliferation And Invasion

Down-regulation of the ErbB3 binding protein 1 in human bladder cancer promotes tumor progression and cell proliferation

2013 ◽  
Vol 40 (5) ◽  
pp. 3799-3805 ◽  
Author(s):  
Hui-chan He ◽  
Xiao-hui Ling ◽  
Jian-guo Zhu ◽  
Xin Fu ◽  
Zhao-dong Han ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Tumor Progression ◽  
Binding Protein ◽  
Human Bladder Cancer ◽  
Down Regulation ◽  
Human Bladder

Curcumin reverses benzidine-induced cell proliferation by suppressing ERK1/2 pathway in human bladder cancer T24 cells

2016 ◽  
Vol 68 (4) ◽  
pp. 215-222 ◽  
Author(s):  
Xin Sun ◽  
Qi-Fei Deng ◽  
Zhao-Feng Liang ◽  
Zhi-Qiang Zhang ◽  
Li Zhao ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
T24 Cells
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