scholarly journals Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow‐derived stromal cells

2020 ◽  
Vol 24 (20) ◽  
pp. 11814-11827
Author(s):  
Mehran Dadras ◽  
Caroline May ◽  
Johannes Maximilian Wagner ◽  
Christoph Wallner ◽  
Mustafa Becerikli ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Proteomic Analysis ◽  
Human Adipose Tissue ◽  
Comparative Proteomic Analysis

scholarly journals Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Alfonso Eirin ◽  
Xiang-Yang Zhu ◽  
Amrutesh S. Puranik ◽  
John R. Woollard ◽  
Hui Tang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis

scholarly journals Infusing Mesenchymal Stromal Cells into Porcine Kidneys during Normothermic Machine Perfusion: Intact MSCs Can Be Traced and Localised to Glomeruli

2019 ◽  
Vol 20 (14) ◽  
pp. 3607 ◽  
Author(s):  
Merel Pool ◽  
Tim Eertman ◽  
Jesus Sierra Parraga ◽  
Nils ’t Hart ◽  
Marieke Roemeling-van Rhijn ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Ex Vivo ◽  
Human Adipose Tissue ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion ◽  
Glomerular Capillaries

Normothermic machine perfusion (NMP) of kidneys offers the opportunity to perform active interventions, such as the addition of mesenchymal stromal cells (MSCs), to an isolated organ prior to transplantation. The purpose of this study was to determine whether administering MSCs to kidneys during NMP is feasible, what the effect of NMP is on MSCs and whether intact MSCs are retained in the kidney and to which structures they home. Viable porcine kidneys were obtained from a slaughterhouse. Kidneys were machine perfused during 7 h at 37 °C. After 1 h of perfusion either 0, 105, 106 or 107 human adipose tissue derived MSCs were added. Additional ex vivo perfusions were conducted with fluorescent pre-labelled bone-marrow derived MSCs to assess localisation and survival of MSCs during NMP. After NMP, intact MSCs were detected by immunohistochemistry in the lumen of glomerular capillaries, but only in the 107 MSC group. The experiments with fluorescent pre-labelled MSCs showed that only a minority of glomeruli were positive for infused MSCs and most of these glomeruli contained multiple MSCs. Flow cytometry showed that the number of infused MSCs in the perfusion circuit steeply declined during NMP to approximately 10%. In conclusion, the number of circulating MSCs in the perfusate decreases rapidly in time and after NMP only a small portion of the MSCs are intact and these appear to be clustered in a minority of glomeruli.

Molecular mechanisms underlying human adipose tissue-derived stromal cells differentiation into a hepatocyte-like phenotype

2010 ◽  
Vol 42 (12) ◽  
pp. 895-901 ◽  
Author(s):  
Nathalie Saulnier ◽  
Anna Chiara Piscaglia ◽  
Maria Ausiliatrice Puglisi ◽  
Marta Barba ◽  
Vincenzo Arena ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Molecular Mechanisms ◽  
Human Adipose Tissue

scholarly journals Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile

2015 ◽  
Vol 24 (23) ◽  
pp. 2822-2840 ◽  
Author(s):  
Lindolfo da Silva Meirelles ◽  
Tathiane Maistro Malta ◽  
Virgínia Mara de Deus Wagatsuma ◽  
Patrícia Viana Bonini Palma ◽  
Amélia Goes Araújo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Mesenchymal Stromal Cells ◽  
Expression Profile ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Similar Gene Expression Profile ◽  
Similar Gene
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