scholarly journals Optimized Langendorff perfusion system for cardiomyocyte isolation in adult mouse heart

2020 ◽  
Vol 24 (24) ◽  
pp. 14619-14625
Author(s):  
Haotong Li ◽  
Chungeng Liu ◽  
Minghui Bao ◽  
Weijing Liu ◽  
Yu Nie ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Mouse Heart ◽  
Perfusion System ◽  
Langendorff Perfusion

scholarly journals Distribution and Structure-Function Relationship of Myosin Heavy Chain Isoforms in the Adult Mouse Heart

2007 ◽  
Vol 282 (33) ◽  
pp. 24057-24064 ◽  
Author(s):  
Maike Krenz ◽  
Sakthivel Sadayappan ◽  
Hanna E. Osinska ◽  
Jeffrey A. Henry ◽  
Samantha Beck ◽  
...  
Keyword(s):  
Structure Function ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Adult Mouse ◽  
Myosin Heavy Chain Isoforms ◽  
Mouse Heart ◽  
Structure Function Relationship ◽  
Function Relationship ◽  
Relationship Of

scholarly journals Isoform-Specific Modulation of Inflammation Induced by Adenoviral Mediated Delivery of Platelet-Derived Growth Factors in the Adult Mouse Heart

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160930 ◽  
Author(s):  
Radiosa Gallini ◽  
Jenni Huusko ◽  
Seppo Ylä-Herttuala ◽  
Christer Betsholtz ◽  
Johanna Andrae
Keyword(s):  
Growth Factors ◽  
Adult Mouse ◽  
Mouse Heart

scholarly journals Cardiomyocyte proliferation and progenitor cell recruitment underlie therapeutic regeneration after myocardial infarction in the adult mouse heart

2013 ◽  
Vol 5 (2) ◽  
pp. 191-209 ◽  
Author(s):  
Konstantinos Malliaras ◽  
Yiqiang Zhang ◽  
Jeffrey Seinfeld ◽  
Giselle Galang ◽  
Eleni Tseliou ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Progenitor Cell ◽  
Adult Mouse ◽  
Mouse Heart ◽  
Cardiomyocyte Proliferation ◽  
Cell Recruitment

scholarly journals Conditional Transgenic Expression of Fibroblast Growth Factor 9 in the Adult Mouse Heart Reduces Heart Failure Mortality After Myocardial Infarction

Circulation ◽  
2011 ◽  
Vol 123 (5) ◽  
pp. 504-514 ◽  
Author(s):  
Mortimer Korf-Klingebiel ◽  
Tibor Kempf ◽  
Klaus-Dieter Schlüter ◽  
Christian Willenbockel ◽  
Torben Brod ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Heart Failure ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Adult Mouse ◽  
Mouse Heart ◽  
Fibroblast Growth ◽  
Transgenic Expression

scholarly journals Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration

2016 ◽  
Vol 2 (11) ◽  
pp. e1600844 ◽  
Author(s):  
William C. W. Chen ◽  
Zhouguang Wang ◽  
Maria Azzurra Missinato ◽  
Dae Woo Park ◽  
Daniel Ward Long ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Adult Mouse ◽  
Contractile Function ◽  
Left Ventricular ◽  
Precursor Cell ◽  
Cell Populations ◽  
Mouse Heart ◽  
Heart Regeneration ◽  
Regenerative Capacity ◽  
Mammalian Heart

Heart attack is a global health problem that leads to significant morbidity, mortality, and health care burden. Adult human hearts have very limited regenerative capability after injury. However, evolutionarily primitive species generally have higher regenerative capacity than mammals. The extracellular matrix (ECM) may contribute to this difference. Mammalian cardiac ECM may not be optimally inductive for cardiac regeneration because of the fibrotic, instead of regenerative, responses in injured adult mammalian hearts. Given the high regenerative capacity of adult zebrafish hearts, we hypothesize that decellularized zebrafish cardiac ECM (zECM) made from normal or healing hearts can induce mammalian heart regeneration. Using zebrafish and mice as representative species of lower vertebrates and mammals, we show that a single administration of zECM, particularly the healing variety, enables cardiac functional recovery and regeneration of adult mouse heart tissues after acute myocardial infarction. zECM-treated groups exhibit proliferation of the remaining cardiomyocytes and multiple cardiac precursor cell populations and reactivation of ErbB2 expression in cardiomyocytes. Furthermore, zECM exhibits pro-proliferative and chemotactic effects on human cardiac precursor cell populations in vitro. These contribute to the structural preservation and correlate with significantly higher cardiac contractile function, notably less left ventricular dilatation, and substantially more elastic myocardium in zECM-treated hearts than control animals treated with saline or decellularized adult mouse cardiac ECM. Inhibition of ErbB2 activity abrogates beneficial effects of zECM administration, indicating the possible involvement of ErbB2 signaling in zECM-mediated regeneration. This study departs from conventional focuses on mammalian ECM and introduces a new approach for cardiac tissue regeneration.

Knockout of Wdr1 results in cardiac hypertrophy and impaired cardiac function in adult mouse heart

Gene ◽  
2019 ◽  
Vol 697 ◽  
pp. 40-47
Author(s):  
Xia Huang ◽  
Ziyi Li ◽  
Jisheng Hu ◽  
Zihao Yang ◽  
Zhongying Liu ◽  
...  
Keyword(s):  
Cardiac Hypertrophy ◽  
Cardiac Function ◽  
Adult Mouse ◽  
Mouse Heart ◽  
Impaired Cardiac Function

Expression of the β (slow)-isoform of MHC in the adult mouse heart causes dominant-negative functional effects

2000 ◽  
Vol 278 (2) ◽  
pp. H412-H419 ◽  
Author(s):  
Jil C. Tardiff ◽  
Timothy E. Hewett ◽  
Stephen M. Factor ◽  
Karen L. Vikstrom ◽  
Jeffrey Robbins ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Adult Mouse ◽  
Systolic Function ◽  
Cardiac Contractility ◽  
Dominant Negative ◽  
Mouse Heart ◽  
Physiological Changes ◽  
Cardiac Chamber ◽  
Ventricular Tissue ◽  
Mouse Lines

α- and β-myosin heavy chain (MHC), the two MHC isoforms expressed in the mammalian heart, differ quantitatively in their enzymatic activities. The MHC composition of the heart can change dramatically in response to numerous stimuli, leading to the hypothesis that changes in cardiac function can be caused by myosin isoform shifts. However, this hypothesis has remained unproven because the stimuli used to generate these shifts are complex and accompanied by many additional physiological changes, including alterations in cardiac mass and geometry. Adult mouse ventricles normally express only α-MHC (the faster motor). To determine whether genetic alteration of the MHC isoform composition in the adult mouse heart would result in changes in cardiac chamber mass and contractility, we established transgenic mouse lines that express a Myc-tagged β-MHC molecule (the slower motor) in adult ventricular tissue, one of which expreses 12% of its myosin as the transgene. There is no evidence of hypertrophy, induction of hypertrophic markers, and no histopathology. Myofibrillar Ca2+-activated ATPase activity is decreased by 23%, and Langendorff preparations demonstrate a significant 15% decrease in systolic function in transgenic hearts. These results suggest that even small shifts in the myosin isoform composition of the myocardium can result in physiologically significant changes in cardiac contractility and could be relevant to cardiovascular disease.

Sign in / Sign up

Export Citation Format

Share Document