scholarly journals SP1‐mediated lncRNA PVT1 modulates the proliferation and apoptosis of lens epithelial cells in diabetic cataract via miR‐214‐3p/MMP2 axis

2020 ◽  
Vol 24 (1) ◽  
pp. 554-561 ◽  
Author(s):  
Jun Yang ◽  
Shaozhen Zhao ◽  
Fang Tian
Keyword(s):  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Diabetic Cataract ◽  
Proliferation And Apoptosis

scholarly journals Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice

2017 ◽  
Vol 37 (4) ◽  
Author(s):  
Kun Zeng ◽  
Qi-Gao Feng ◽  
Bao-Tao Lin ◽  
Da-Hui Ma ◽  
Chun-Min Liu
Keyword(s):  
Epithelial Cells ◽  
Protein Concentration ◽  
Negative Control ◽  
Lens Epithelial Cells ◽  
Male Mice ◽  
Healthy Male ◽  
Diabetic Cataract ◽  
Apoptosis Index ◽  
Proliferation And Apoptosis ◽  
Sirt1 Gene

Our study aimed at exploring the effects of miR-211 on the proliferation and apoptosis of lens epithelial cells in diabetic cataract mice by targetting NAD+-dependent histone deacetylase sirtulin 1 (SIRT1). Healthy male mice were assigned into normal and diabetic cataract groups. Blood glucose, lens turbidity, and apoptosis were measured. Lens epithelial cells were classified into the normal, blank, negative control (NC), miR-211 mimics, miR-211 inhibitors, siRNA-SIRT1, and miR-211 inhibitors + siRNA-SIRT1 groups. MiR-211, Bcl-2, Bax, p53, and SIRT1 expressions of each group were detected. Cell proliferation, cycle and apoptosis were tested by MTT assay and flow cytometry. MiR-211 can specifically bind to SIRT1 according to the luciferase system. SIRT1 protein concentration was strongly positive in normal mice and weakly positive in diabetic cataract mice. Apoptosis index of diabetic cataract mice was higher than the normal mice. Compared with normal mice, the expressions of miR-211, Bax, and p53 increased in diabetic cataract mice, while the Bcl-2 and SIRT1 expressions decreased. In comparison with the blank and NC groups, the expressions of miR-211, Bax, and p53 increased, while Bcl-2 and SIRT1 expressions decreased, and the proliferation decreased and apoptosis rate increased in the miR-211 mimics and siRNA-SIRT1 groups; the results were contradicting for the miR-211 inhibitor group. MiR-211 could promote apoptosis and inhibit proliferation of lens epithelial cells in diabetic cataract mice by targetting SIRT1.

Expression of p53 and caspase-8 in lens epithelial cells of diabetic cataract

2014 ◽  
Vol 40 (7) ◽  
pp. 1102-1108 ◽  
Author(s):  
Sung A. Lim ◽  
Choun-Ki Joo ◽  
Man Soo Kim ◽  
Sung Kun Chung
Keyword(s):  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Caspase 8 ◽  
Diabetic Cataract

Orai3 exacerbates apoptosis of lens epithelial cells by disrupting calcium homeostasis in diabetic cataract

Toxicon ◽  
2019 ◽  
Vol 158 ◽  
pp. S85
Author(s):  
Yong Wang ◽  
Suwen Bai ◽  
Juan Du ◽  
Lin Xia ◽  
Jizheng Guo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Calcium Homeostasis ◽  
Lens Epithelial Cells ◽  
Diabetic Cataract

scholarly journals Orai3 exacerbates apoptosis of lens epithelial cells by disrupting Ca 2+ homeostasis in diabetic cataract

2021 ◽  
Vol 11 (3) ◽  
Author(s):  
Yong Wang ◽  
Suwen Bai ◽  
Ru Zhang ◽  
Lin Xia ◽  
Linghui Chen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Diabetic Cataract
Sign in / Sign up

Export Citation Format

Share Document