scholarly journals Optical fluorescence imaging with shortwave infrared light emitter nanomaterials for in vivo cell tracking in regenerative medicine

2019 ◽  
Vol 23 (12) ◽  
pp. 7905-7918 ◽  
Author(s):  
Leyla Fath‐Bayati ◽  
Mohammad Vasei ◽  
Ehsan Sharif‐Paghaleh
Keyword(s):  
Regenerative Medicine ◽  
Fluorescence Imaging ◽  
Cell Tracking ◽  
Infrared Light ◽  
Light Emitter ◽  
Optical Fluorescence ◽  
Shortwave Infrared ◽  
Optical Fluorescence Imaging

scholarly journals Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared

2018 ◽  
Vol 115 (37) ◽  
pp. 9080-9085 ◽  
Author(s):  
Jessica A. Carr ◽  
Marianne Aellen ◽  
Daniel Franke ◽  
Peter T. C. So ◽  
Oliver T. Bruns ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Fluorescence Imaging ◽  
Biological Tissue ◽  
Ex Vivo ◽  
Scattered Light ◽  
Image Contrast ◽  
Fluorescence Image ◽  
Tissue Phantoms ◽  
Shortwave Infrared

Recent technology developments have expanded the wavelength window for biological fluorescence imaging into the shortwave infrared. We show here a mechanistic understanding of how drastic changes in fluorescence imaging contrast can arise from slight changes of imaging wavelength in the shortwave infrared. We demonstrate, in 3D tissue phantoms and in vivo in mice, that light absorption by water within biological tissue increases image contrast due to attenuation of background and highly scattered light. Wavelengths of strong tissue absorption have conventionally been avoided in fluorescence imaging to maximize photon penetration depth and photon collection, yet we demonstrate that imaging at the peak absorbance of water (near 1,450 nm) results in the highest image contrast in the shortwave infrared. Furthermore, we show, through microscopy of highly labeled ex vivo biological tissue, that the contrast improvement from water absorption enables resolution of deeper structures, resulting in a higher imaging penetration depth. We then illustrate these findings in a theoretical model. Our results suggest that the wavelength-dependent absorptivity of water is the dominant optical property contributing to image contrast, and is therefore crucial for determining the optimal imaging window in the infrared.

Development of a CCD-based optical fluorescence imaging system

2004 ◽  
Author(s):  
Yuhua Li ◽  
David Tang ◽  
Jason Y. Chen ◽  
Jessica Wong ◽  
Hong Liu
Keyword(s):  
Fluorescence Imaging ◽  
Imaging System ◽  
Optical Fluorescence ◽  
Fluorescence Imaging System ◽  
Optical Fluorescence Imaging
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