scholarly journals miR‐20a‐5p regulates pulmonary surfactant gene expression in alveolar type II cells

2019 ◽  
Vol 23 (11) ◽  
pp. 7664-7672 ◽  
Author(s):  
Yongjian Gong ◽  
Weidong Xu ◽  
Yang Chen ◽  
Yun Liu ◽  
Yuan Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pulmonary Surfactant ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells

A human SP-C promoter fragment targets α1-proteinase inhibitor gene expression to lung alveolar type II cells in transgenic mice

1996 ◽  
Vol 5 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Eric Degryse ◽  
Maria M. De Santi ◽  
Mireille Dietrich ◽  
Dalila Ali Hadji ◽  
Jean François Spetz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Proteinase Inhibitor ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Promoter Fragment ◽  
Inhibitor Gene

scholarly journals Effects of bone marrow-derived mesenchymal stromal cells on gene expression in human alveolar type II cells exposed to TNF-α , IL-1β , and IFN-γ

2018 ◽  
Vol 6 (16) ◽  
pp. e13831 ◽  
Author(s):  
Matthew Schwede ◽  
Erin M. Wilfong ◽  
Rachel L. Zemans ◽  
Patty J. Lee ◽  
Claudia dos Santos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Tnf Α ◽  
Ifn Γ

Role of Peripheral Benzodiazepine Receptors on Secretion of Surfactant in Guinea Pig Alveolar Type II Cells

1999 ◽  
Vol 19 (5) ◽  
pp. 461-471 ◽  
Author(s):  
Salil K. Das ◽  
Shyamali Mukherjee
Keyword(s):  
Pulmonary Surfactant ◽  
Benzodiazepine Receptors ◽  
Alveolar Epithelium ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Peripheral Tissues ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Peripheral Benzodiazepine Receptors

Granular type II cells located in the alveolar epithelium synthesize and secrete pulmonary surfactant and have specialized ion transport system. Alveolar type II cells are stimulated to secrete pulmonary surfactant by a variety of agonists. One mechanism by which extracellular signals are perceived by cells is the mobilization of intracellular Ca2+. Peripheral benzodiazepine receptors (PBRs) are present in both peripheral tissues and central nervous system. We have previously reported the presence of high density PBRs in lung and alveolar type II cells. It is known that both PBRs and beta-adrenergic receptors (beta-ARs) play an important role in cellular Ca2+ transport. Furthermore, we have suggested earlier that PBRs are someway functionally associated with the beta-ARs. The objective of the present study was to determine whether PBRs play any role in the secretion of surfactant by alveolar type II cells. Alveolar type II cells were isolated from normal weanling guinea pigs by panning method and incubated with 3H-palmitic acid in minimum essential medium to synthesize labelled dipalmitoyl phosphatidylcholine (DPPC). After washing, the cells were treated at 37°C for one hour with 10 μM isoproterenol (IP) in the presence and absence of 10 μM Ro 5-4864, an agonist for PBRs. After one hour, the release of labelled DPPC in the medium was analyzed. The control cells released DPPC without any addition of a ligand. However, the treatment of cells with IP, Ro 5-4864 and IP + Ro 5-4864 caused 24, 52 and 171% increase in the secretion of DPPC, respectively. In another experiment, type II cells were loaded with Fura-2 dye and treated with either IP or epineprine or Ro 5-4864. Both isoproterenol and epinephrine caused a significant increase in the level of cytosolic free Ca2+. However, Ro 5-4864 caused not only a decrease in the level of cytosolic free Ca2+ but also counteracted the stimulatory effect of IP. This may suggest that while ligands for ARs stimulate Ca2+ release into cytosol, the ligand for PBRs stimulates efflux of Ca2+ in alveolar type cells. Thus, the increased secretion of surfactant by the ligand of PBRs in alveolar type II cells may be mediated through its effects on increased Ca2+ efflux.

Effect of secretagogues on cytoplasmic free calcium in alveolar type II epithelial cells

1987 ◽  
Vol 253 (5) ◽  
pp. C679-C686 ◽  
Author(s):  
K. Sano ◽  
D. R. Voelker ◽  
R. J. Mason
Keyword(s):  
Pulmonary Surfactant ◽  
Calcium Concentration ◽  
Free Calcium ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Surfactant Secretion ◽  
Free Calcium Concentration ◽  
Cytoplasmic Free Calcium

Pulmonary surfactant is synthesized and secreted by alveolar type II epithelial cells. Although intracellular calcium and other second messengers have been implicated in secretion by type II cells, this is the first report on measurement of cytoplasmic free calcium concentration ([Ca2+]i). Known secretagogues, 12-O-tetradecanoylphorbol-13-acetate (TPA) and terbutaline, were tested to see if they caused rapid increases in cytoplasmic calcium. Ionomycin, a calcium ionophore, was used to increase cytoplasmic free calcium concentration, to determine if a rapid increase in cytoplasmic free calcium would stimulate secretion, and to measure interactions with other secretagogues. Ionomycin increased both [Ca2+]i and pulmonary surfactant secretion from alveolar type II cells. A low concentration of ionomycin (100 nM) greatly enhanced secretion stimulated by terbutaline or by 8-bromo-cAMP but only had an additive effect on secretion stimulated by TPA. Terbutaline transiently increased [Ca2+]i by 24% over control basal condition, and the increase in [Ca2+]i produced by terbutaline occurred in the absence of extracellular calcium. TPA itself did not change [Ca2+]i. However, TPA completely inhibited the terbutaline-induced increase of [Ca2+]i but not the increase due to ionomycin. When alveolar type II cells were loaded with 2-(2-bis-[carboxymethyl]-amino-5-methyl-phenoxy)-methyl-6-methoxy-8-bis carboxymethylaminoquinoline (quin2) in calcium-free buffer, [Ca2+]i decreased from 143 +/- 10 to 31 +/- 8 nM. Lowering [Ca2+]i inhibited TPA- or terbutaline-induced secretion by 22 and 40%, respectively. Although the precise role of cytoplasmic free calcium on surfactant secretion cannot be established on the basis of current data, our results indicate that an increase in cytoplasmic free calcium produced by ionomycin stimulates secretion and that an increase in [Ca2+]i affects cAMP-induced secretion more than protein kinase C-mediated secretion in alveolar type II cells.

scholarly journals Heparin Inhibits DNA Synthesis and Gene Expression in Alveolar Type II Cells

2002 ◽  
Vol 27 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Cheng-ming Li ◽  
Donna Newman ◽  
Jody Khosla ◽  
Philip L. Sannes
Keyword(s):  
Gene Expression ◽  
Dna Synthesis ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells

scholarly journals Pulmonary Surfactant Preserves Viability of Alveolar Type II Cells Exposed to Polymyxin B In Vitro

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e62105 ◽  
Author(s):  
Guido Stichtenoth ◽  
Egbert Herting ◽  
Mario Rüdiger ◽  
Andreas Wemhöner
Keyword(s):  
Pulmonary Surfactant ◽  
Polymyxin B ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells
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