scholarly journals Microenvironment‐induced PIM kinases promote CXCR 4‐triggered mTOR pathway required for chronic lymphocytic leukaemia cell migration

2018 ◽  
Vol 22 (7) ◽  
pp. 3548-3559 ◽  
Author(s):  
Emilia Białopiotrowicz ◽  
Patryk Górniak ◽  
Monika Noyszewska‐Kania ◽  
Bartosz Puła ◽  
Hanna Makuch‐Łasica ◽  
...  
Keyword(s):  
Cell Migration ◽  
Chronic Lymphocytic Leukaemia ◽  
Lymphocytic Leukaemia ◽  
Mtor Pathway ◽  
Leukaemia Cell ◽  
Pim Kinases ◽  
Chronic Lymphocytic Leukaemia Cell

scholarly journals Establishment and Characterization of PCL12, a Novel CD5+ Chronic Lymphocytic Leukaemia Cell Line

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130195 ◽  
Author(s):  
Andreas Agathangelidis ◽  
Lydia Scarfò ◽  
Federica Barbaglio ◽  
Benedetta Apollonio ◽  
Maria Teresa Sabrina Bertilaccio ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Cell Line ◽  
Lymphocytic Leukaemia ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Chronic Lymphocytic Leukaemia Cell

scholarly journals Correction: Establishment and Characterization of PCL12, a Novel CD5+ Chronic Lymphocytic Leukaemia Cell Line

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0134748
Author(s):  
Andreas Agathangelidis ◽  
Lydia Scarfò ◽  
Federica Barbaglio ◽  
Benedetta Apollonio ◽  
Maria Teresa Sabrina Bertilaccio ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Cell Line ◽  
Lymphocytic Leukaemia ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Chronic Lymphocytic Leukaemia Cell

scholarly journals BAG3 protein controls B-chronic lymphocytic leukaemia cell apoptosis

2003 ◽  
Vol 10 (3) ◽  
pp. 383-385 ◽  
Author(s):  
M F Romano ◽  
M Festa ◽  
G Pagliuca ◽  
R Lerose ◽  
R Bisogni ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Cell Apoptosis ◽  
Lymphocytic Leukaemia ◽  
Leukaemia Cell ◽  
Chronic Lymphocytic Leukaemia Cell

scholarly journals The 5-lipoxygenase-activating protein (FLAP) inhibitor, MK886, induces apoptosis independently of FLAP

1999 ◽  
Vol 340 (2) ◽  
pp. 371-375 ◽  
Author(s):  
Kaushik DATTA ◽  
Shyam S. BISWAL ◽  
James P. KEHRER
Keyword(s):  
Cell Line ◽  
Lymphocytic Leukaemia ◽  
Fatty Acid Binding Proteins ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Fatty Acid Binding ◽  
Oxidative Reactions ◽  
Chronic Lymphocytic Leukaemia Cell ◽  
Induced Apoptosis

The ability of various inhibitors of lipoxygenase (LOX) enzymes and 5-lipoxygenase-activating protein (FLAP) to induce apoptosis has implicated these pathways in the mechanism(s) of this form of cell death. Although FLAP plays an important role in 5-LOX activity, this protein is found at high levels in some cells lacking LOX, suggesting it might mediate other effects. Furthermore, the concentration of MK886, a FLAP inhibitor, required to induce apoptosis is ≈ 100-fold more than that required to inhibit LOX, and this compound remains effective in cells lacking LOX. The present study examines the role of FLAP in MK886-induced apoptosis. MK886 induced apoptosis in WSU cells, a human chronic lymphocytic leukaemia cell line that lacks FLAP protein and mRNA, suggesting that this agent is acting independently of FLAP. This conclusion was further supported by the fact that a more specific FLAP inhibitor, MK591, induced only minimal apoptosis in FL5.12 cells, a murine prolymphoid cell line containing FLAP. The role of FLAP was examined more directly by decreasing its expression by more than 50% in FL5.12 cells treated with 10 μM of an antisense oligonucleotide for 48 h. This change in FLAP was not accompanied by any increase in apoptosis. Furthermore, FLAP-depleted cells exhibited the same level of apoptosis 8 h after treatment with 10 μM MK886, as did control cells. The increased fluorescence seen in MK886-treated cells loaded with carboxydichlorofluorescein indicates that oxidative reactions are stimulated by this compound, possibly via the release of fatty acids from fatty acid-binding proteins and their subsequent oxidation.

scholarly journals Towards a Better Characterisation of Leukemic Cells in Chronic Lymphocytic Leukaemia: Cell-Size Heterogeneity Reflects Their Activation Status and Migratory Abilities

Cancers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 4922
Author(s):  
Gayane Manukyan ◽  
Zuzana Mikulkova ◽  
Peter Turcsanyi ◽  
Jakub Savara ◽  
Markéta Trajerová ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Cell Size ◽  
Lymphocytic Leukaemia ◽  
Leukemic Cells ◽  
Significant Heterogeneity ◽  
Migration Activity ◽  
Hla Dr ◽  
Chronic Lymphocytic Leukaemia Cell ◽  
Size Heterogeneity ◽  
Activation Status

Chronic lymphocytic leukaemia (CLL) is a genetically, morphologically and phenotypically heterogeneous chronic disease with clinical variability between patients. Whether the significant heterogeneity of cell size within the CLL population contributes to the heterogeneous features of this disease has not been investigated. The present study aimed to characterise the phenotypic and functional properties of two subpopulations of typical CLL cells that differ in cell size: small (s-CLL) and large (l-CLL) CLL cells delineated by forward scatter cytometry. The s-CLL cells were characterised by the CD5lowCXCR4hi phenotype, while the l-CLL cells were characterised by the CD5hiCXCR4dim phenotype and indicated a higher expression of CXCR3, CD20, CD38 and HLA-DR. The l-CLL cells displayed higher migration activity towards CXCL12, a tendency towards a higher proliferation rate and an increased capacity to produce IgM in the presence of CpG compared with s-CLL cells. When stimulated with CpG and CXCL12, l-CLL cells were characterised by a higher polarisation phenotype and motility than s-CLL cells. Our study revealed that the differences in CLL cell size reflected their activation status, polarisation and migratory abilities. Our data provide evidence of the importance of cell-size heterogeneity within a CLL pool and the dynamics of cell-size changes for disease pathogenesis, thus deserving further investigation.

Autologous T lymphocytes recognize the tumour-derived immunoglobulin VH-CDR3 region in patients with B-cell chronic lymphocytic leukaemia

2000 ◽  
Vol 111 (1) ◽  
pp. 230-238 ◽  
Author(s):  
Mohammad Reza Rezvany ◽  
Mahmood Jeddi-Tehrani ◽  
Hodjattallah Rabbani ◽  
Ulla Ruden ◽  
Lennart Hammarstrom ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Chronic Lymphocytic Leukaemia ◽  
B Cell ◽  
Lymphocytic Leukaemia ◽  
Cell Chronic Lymphocytic Leukaemia ◽  
Cdr3 Region

Pharmacokinetics of Chlorambucil in Patients with Chronic Lymphocytic Leukaemia: Comparison of Different Days, Cycles and Doses

2000 ◽  
Vol 87 (5) ◽  
pp. 223-228 ◽  
Author(s):  
Raija Silvennoinen ◽  
Kimmo Malminiemi ◽  
Outi Malminiemi ◽  
Erkki Seppala ◽  
Juhani Vilpo
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Lymphocytic Leukaemia
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