scholarly journals Identification of stably expressed reference small non‐coding RNA s for micro RNA quantification in high‐grade serous ovarian carcinoma tissues

2016 ◽  
Vol 20 (12) ◽  
pp. 2341-2348 ◽  
Author(s):  
Eliana Bignotti ◽  
Stefano Calza ◽  
Renata A. Tassi ◽  
Laura Zanotti ◽  
Elisabetta Bandiera ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Micro Rna ◽  
High Grade ◽  
Rna Quantification ◽  
Serous Ovarian Carcinoma ◽  
Non Coding Rna

scholarly journals Long Non-Coding RNA MAGI2-AS3 is a New Player with a Tumor Suppressive Role in High Grade Serous Ovarian Carcinoma

Cancers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2008 ◽  
Author(s):  
Priyanka Gokulnath ◽  
Tiziana de Cristofaro ◽  
Ichcha Manipur ◽  
Tina Di Palma ◽  
Amata Amy Soriano ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
High Grade ◽  
Mirna Sponge ◽  
Downstream Signaling ◽  
Cellular Processes ◽  
Serous Ovarian Carcinoma ◽  
Non Coding Rna ◽  
Important Player ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

High-Grade Serous Ovarian Carcinoma (HGSC) is the most incidental and lethal subtype of epithelial ovarian cancer (EOC) with a high mortality rate of nearly 65%. Recent findings aimed at understanding the pathogenesis of HGSC have attributed its principal source as the Fallopian Tube (FT). To further comprehend the exact mechanism of carcinogenesis, which is still less known, we performed a transcriptome analysis comparing FT and HGSC. Our study aims at exploring new players involved in the development of HGSC from FT, along with their signaling network, and we chose to focus on non-coding RNAs. Non-coding RNAs (ncRNAs) are increasingly observed to be the major regulators of several cellular processes and could have key functions as biological markers, as well as even a therapeutic approach. The most physiologically relevant and significantly dysregulated non-coding RNAs were identified bioinformatically. After analyzing the trend in HGSC and other cancers, MAGI2-AS3 was observed to be an important player in EOC. We assessed its tumor-suppressive role in EOC by means of various assays. Further, we mapped its signaling pathway using its role as a miRNA sponge to predict the miRNAs binding to MAGI2AS3 and showed it experimentally. We conclude that MAGI2-AS3 acts as a tumor suppressor in EOC, specifically in HGSC by sponging miR-15-5p, miR-374a-5p and miR-374b-5p, and altering downstream signaling of certain mRNAs through a ceRNA network.

scholarly journals Long Non-Coding RNA HAND2-AS1 Acts as a Tumor Suppressor in High-Grade Serous Ovarian Carcinoma

2020 ◽  
Vol 21 (11) ◽  
pp. 4059
Author(s):  
Priyanka Gokulnath ◽  
Tiziana de Cristofaro ◽  
Ichcha Manipur ◽  
Tina Di Palma ◽  
Amata Amy Soriano ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Ovarian Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Chemotherapeutic Agents ◽  
Sequencing Analysis ◽  
High Grade ◽  
Serous Ovarian Carcinoma ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Long non-coding RNAs (lncRNAs) are increasingly being identified as crucial regulators in pathologies like cancer. High-grade serous ovarian carcinoma (HGSC) is the most common subtype of ovarian cancer (OC), one of the most lethal gynecological malignancies. LncRNAs, especially in cancers such as HGSC, could play a valuable role in diagnosis and even therapy. From RNA-sequencing analysis performed between an OC cell line, SKOV3, and a Fallopian Tube (FT) cell line, FT194, an important long non-coding RNA, HAND2 Anti sense RNA 1 (HAND2-AS1), was observed to be significantly downregulated in OCs when compared to FT. Its downregulation in HGSC was validated in different datasets and in a panel of HGSC cell lines. Furthermore, this study shows that the downregulation of HAND2-AS1 is caused by promoter hypermethylation in HGSC and behaves as a tumor suppressor in HGSC cell lines. Since therapeutic relevance is of key importance in HGSC research, for the first time, HAND2-AS1 upregulation was demonstrated to be one of the mechanisms through which HDAC inhibitor Panobinostat could be used in a strategy to increase HGSC cells’ sensitivity to chemotherapeutic agents currently used in clinical trials. To unravel the mechanism by which HAND2-AS1 exerts its role, an in silico mRNA network was constructed using mRNAs whose expressions were positively and negatively correlated with this lncRNA in HGSC. Finally, a putative ceRNA network with possible miRNA targets of HAND2-AS1 and their mRNA targets was constructed, and the enriched Gene Ontology (GO) biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified.

Neoplastic cellularity is associated with clinical and molecular features of high-grade serous ovarian carcinoma

2015 ◽  
Vol 139 (1) ◽  
pp. 196
Author(s):  
C. Morse ◽  
B. Norquist ◽  
S. Bernards ◽  
M. Harrell ◽  
K. Agnew ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
High Grade ◽  
Molecular Features ◽  
Serous Ovarian Carcinoma

scholarly journals Retinoblastoma pathway deregulatory mechanisms determine clinical outcome in high-grade serous ovarian carcinoma

2013 ◽  
Vol 27 (7) ◽  
pp. 991-1001 ◽  
Author(s):  
Anca Milea ◽  
Sophia HL George ◽  
Donco Matevski ◽  
Haiyan Jiang ◽  
Mary Madunic ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Ovarian Carcinoma ◽  
High Grade ◽  
Serous Ovarian Carcinoma

MiR-182 overexpression in tumourigenesis of high-grade serous ovarian carcinoma

2012 ◽  
Vol 228 (2) ◽  
pp. 204-215 ◽  
Author(s):  
Zhaojian Liu ◽  
Jinsong Liu ◽  
Miguel F Segura ◽  
Changshun Shao ◽  
Peng Lee ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
High Grade ◽  
Serous Ovarian Carcinoma

scholarly journals Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jennifer Ducie ◽  
Fanny Dao ◽  
Michael Considine ◽  
Narciso Olvera ◽  
Patricia A. Shaw ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Molecular Analysis ◽  
High Grade ◽  
Serous Ovarian Carcinoma

Modeling High-grade serous ovarian carcinoma using a combination of in vivo fallopian tube electroporation and CRISPR-Cas9-mediated genome editing

2021 ◽  
pp. canres.1518.2020
Author(s):  
Katie Teng ◽  
Matthew J Ford ◽  
Keerthana Harwalkar ◽  
YuQi Li ◽  
Alain Sarabia Pacis ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Genome Editing ◽  
Fallopian Tube ◽  
High Grade ◽  
Serous Ovarian Carcinoma
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