scholarly journals Selection of cell‐type specific antibodies on tissue‐sections using phage display

2015 ◽  
Vol 19 (8) ◽  
pp. 1939-1948 ◽  
Author(s):  
Simon Asbjørn Larsen ◽  
Theresa Meldgaard ◽  
Simon Lykkemark ◽  
Ole Aalund Mandrup ◽  
Peter Kristensen
Keyword(s):  
Phage Display ◽  
Cell Type ◽  
Specific Antibodies ◽  
Tissue Sections ◽  
Cell Type Specific ◽  
Selection Of

Charges drive selection of specific antibodies by phage display

2010 ◽  
Vol 353 (1-2) ◽  
pp. 24-30 ◽  
Author(s):  
Helena Persson ◽  
Jonas Persson ◽  
Lena Danielsson ◽  
Mats Ohlin
Keyword(s):  
Phage Display ◽  
Specific Antibodies ◽  
Selection Of

scholarly journals First person – Martín Baccino-Calace

Biology Open ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. bio055483
Keyword(s):  
Graduate Student ◽  
Life Sciences ◽  
Early Career ◽  
First Person ◽  
Cell Type ◽  
Synaptic Physiology ◽  
Early Career Researchers ◽  
Cell Type Specific ◽  
University Of Zurich ◽  
Selection Of

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Martín Baccino-Calace is first author on ‘Compartment and cell-type specific hypoxia responses in the developing Drosophila brain’, published in BiO. Martín conducted the research described in this article while a master's student in Rafael Cantera's lab at the Department of Neurodevelopment Biology, IIBCE, Uruguay. He is now a graduate student in the lab of Martin Müller at the Department of Molecular Life Sciences, University of Zurich, Switzerland, investigating synaptic physiology.

Tissue Biomarkers and Neuroprotection

2017 ◽  
Author(s):  
Axel Petzold
Keyword(s):  
Neurocritical Care ◽  
Neurological Complications ◽  
Imaging Biomarker ◽  
Optimal Timing ◽  
Clinical Settings ◽  
Structural Imaging ◽  
Cell Type ◽  
The Central Nervous System ◽  
Cell Type Specific ◽  
Selection Of

This chapter reviews the evidence for using biomarkers to measure damage to the central nervous system (CNS) in neurocritical care and perioperative medicine. A conceptual framework is provided to guide the optimal timing of blood, cerebrospinal fluid, and structural imaging biomarker assessment in relation to the onset of injury. A selection of well-validated, cell type–specific biomarkers of CNS tissue damage are reviewed, including their composition, biokinetics, and specificity for neurons, axons, astrocytes, and microglia. Each of these biomarkers will be reviewed in the pertinent clinical settings of stroke, traumatic brain injury, cardiac arrest, Guillain-Barré syndrome, and neurological complications of critical illness and surgery.

scholarly journals Deterministic splicing ofDscam2is regulated by Muscleblind

2019 ◽  
Vol 5 (1) ◽  
pp. eaav1678 ◽  
Author(s):  
Joshua Shing Shun Li ◽  
S. Sean Millard
Keyword(s):  
Alternative Splicing ◽  
Transmembrane Proteins ◽  
Protein Isoforms ◽  
Cell Type ◽  
Binding Properties ◽  
Unique Protein ◽  
Regulated Expression ◽  
Cell Type Specific ◽  
Isoform B ◽  
Selection Of

Alternative splicing increases the proteome diversity crucial for establishing the complex circuitry between trillions of neurons. To provide individual cells with different repertoires of protein isoforms, however, this process must be regulated. Previously, we found that the mutually exclusive alternative splicing ofDrosophila Dscam2produces two isoforms (A and B) with unique binding properties. This splicing event is cell type specific, and the transmembrane proteins that it generates are crucial for the development of axons, dendrites, and synapses. Here, we show that Muscleblind (Mbl) controlsDscam2alternative splicing. Mbl represses isoform A and promotes the selection of isoform B.Mblmutants exhibit phenotypes also observed in flies engineered to express a singleDscam2isoform. Consistent with this,mblexpression is cell type specific and correlates with the splicing of isoform B. Our study demonstrates how the regulated expression of a splicing factor is sufficient to provide neurons with unique protein isoforms crucial for development.

Abstract 12105: Targeted Delivery of Therapeutic Agents After Myocardial Infarction

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Siva Sai Krishna Dasa ◽  
Marc E Seamen ◽  
Brent A French ◽  
Kimberly A Kelly
Keyword(s):  
Myocardial Infarction ◽  
Phage Display ◽  
Targeted Delivery ◽  
Cell Types ◽  
Phage Display Library ◽  
Border Zone ◽  
Cell Type ◽  
Cellular Targets ◽  
Cell Type Specific

Introduction: Current therapies for heart failure (HF) after myocardial infarction (MI) only slow the progression of LV remodeling and have little capacity to regenerate cardiac muscle lost to MI. To expedite targeted delivery of regenerative therapies post-MI, we hypothesized that suitable targets could be identified by biopanning the heart with a phage display library in a mouse model of MI. Methods: A phage display library was biopanned in vivo to identify peptides specific for the infarct/border zone 4 days post-MI. Fluorescence molecular tomography (FMT) followed by tissue immunofluorescence was performed to interrogate the specificity of phage groups and individual clones with targeted phage at VT680 and neg control phage at VT750. The VT680 fluorophore on the targeted phage clones was then used to identify the cellular targets of those clones by counter-staining with antibodies against cell types of interest. Results: We identified phage clones specific for endothelium, cardiomyocytes, inflammatory fibroblasts and c-Kit+ cells present in the border zone post-MI. Liposomes conjugated with different cell type specific peptides had different accumulation rates in the post-infarct heart as visualized by FMT imaging (Fig. 1a). Immunofluorescence analysis demonstrated cell-type specific association of the targeted liposomes with cells expressing c-Kit, CD31 and Hrnr (Figs. 1b&c). We have also been successful in remote loading of anti-apoptotic and immune suppresive drugs into these liposomes and are currently studying their effect in mice after MI. Conclusions: Peptides identified by this screen enable the targeting of different cell types present in the border zone with different drugs. Identifying the molecular binding partners for these peptides may yield insight into the various events/pathways that evolve after a myocardial infarction.

The appearance of neural and glial cell markers during early development of the nervous system in the amphibian embryo

Development ◽  
1989 ◽  
Vol 107 (1) ◽  
pp. 43-54 ◽  
Author(s):  
N.J. Messenger ◽  
A.E. Warner
Keyword(s):  
Nervous System ◽  
Glial Cell ◽  
Glial Cells ◽  
Cell Type ◽  
Neurofilament Protein ◽  
Amphibian Embryo ◽  
Specific Antibodies ◽  
Cell Type Specific ◽  
Radial Glial ◽  
The Brain

Cell-type-specific antibodies have been used to follow the appearance of neurones and glia in the developing nervous system of the amphibian embryo. Differentiated neurones were recognized with antibodies against neurofilament protein while glial cells were identified with antibodies against glial fibrillary acidic protein (GFAP). The appearance of neurones containing the neurotransmitters 5-hydroxytryptamine and dopamine has been charted also. In Xenopus, neurofilament protein in developing neurones was observed occasionally at NF stage 21 and was present reliably in the neural tube and in caudal regions of the brain at stage 23. Antibodies to the low molecular weight fragment of the neurofilament triplet recognized early neurones most reliably. Radial glial cells, identified with GFAP antibody, were identified from stage 23 onwards in the neural tube and caudal regions of the brain. In the developing spinal cord, GFAP staining was apparent throughout the cytoplasm of each radial glial cell. In the brain, the peripheral region only of each glial cell contained GFAP. By stage 36, immunohistochemically recognizable neurones and glia were present throughout the nervous system. In the axolotl, by stage 36 the pattern of neural and glial staining was identical to that observed in Xenopus. GFAP staining of glial cells was obvious at stage 23, although neuronal staining was clearly absent. This implies that glial cells differentiate before neurones. 5-HT-containing cell bodies were first observed in caudal regions of the developing brain on either side of the midline at stage 26. An extensive network of 5-HT neurones appeared gradually, with a substantial subset crossing to the opposite side of the brain through the developing optic chiasma. 5,7-dihydroxytryptamine prevented the appearance of 5-HT. Depletion of 5-HT had little effect on development or swimming behaviour. Dopamine-containing neurones in the brain first differentiated at stage 35–36 and gradually increased in number up to stage 45–47, the latest stage examined. The functional role of 5-HT- or dopamine-containing neurones remains to be elucidated. We conclude that cell-type-specific antibodies can be used to identify neurones and glial cells at early times during neural development and may be useful tools in circumstances where functional identification is difficult.

De novo identification of cell-type specific antibody-antigen pairs by phage display subtraction

2001 ◽  
Vol 268 (10) ◽  
pp. 3099-3107 ◽  
Author(s):  
Brian Stausbøl-Grøn ◽  
Kim Bak Jensen ◽  
Kristian Hobolt Jensen ◽  
Morten Østergaard Jensen ◽  
Brian F. C. Clark
Keyword(s):  
Phage Display ◽  
Specific Antibody ◽  
De Novo ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals A viral toolkit for recording transcription factor–DNA interactions in live mouse tissues

2020 ◽  
Vol 117 (18) ◽  
pp. 10003-10014 ◽  
Author(s):  
Alexander J. Cammack ◽  
Arnav Moudgil ◽  
Jiayang Chen ◽  
Michael J. Vasek ◽  
Mark Shabsovich ◽  
...  
Keyword(s):  
Regulation Of Gene Expression ◽  
Cell Type ◽  
Cell Type Specificity ◽  
Live Animal ◽  
Specific Antibodies ◽  
Genome Wide ◽  
Mouse Tissues ◽  
Conditional Expression ◽  
Cell Type Specific ◽  
Live Mouse

Transcription factors (TFs) enact precise regulation of gene expression through site-specific, genome-wide binding. Common methods for TF-occupancy profiling, such as chromatin immunoprecipitation, are limited by requirement of TF-specific antibodies and provide only end-point snapshots of TF binding. Alternatively, TF-tagging techniques, in which a TF is fused to a DNA-modifying enzyme that marks TF-binding events across the genome as they occur, do not require TF-specific antibodies and offer the potential for unique applications, such as recording of TF occupancy over time and cell type specificity through conditional expression of the TF–enzyme fusion. Here, we create a viral toolkit for one such method, calling cards, and demonstrate that these reagents can be delivered to the live mouse brain and used to report TF occupancy. Further, we establish a Cre-dependent calling cards system and, in proof-of-principle experiments, show utility in defining cell type-specific TF profiles and recording and integrating TF-binding events across time. This versatile approach will enable unique studies of TF-mediated gene regulation in live animal models.

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