scholarly journals Formation of functional gap junctions in amniotic fluid‐derived stem cells induced by transmembrane co‐culture with neonatal rat cardiomyocytes

2013 ◽  
Vol 17 (6) ◽  
pp. 774-781 ◽  
Author(s):  
Jennifer Petsche Connell ◽  
Emily Augustini ◽  
Kenneth J. Moise ◽  
Anthony Johnson ◽  
Jeffrey G. Jacot
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Gap Junctions ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity

Cytotherapy ◽  
2009 ◽  
pp. 1-15 ◽  
Author(s):  
Remco Koninckx ◽  
Karen Hensen ◽  
Annick Daniëls ◽  
Marjan Moreels ◽  
Ivo Lambrichts ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Neonatal Rat ◽  
Human Bone Marrow ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity

Cytotherapy ◽  
2009 ◽  
Vol 11 (6) ◽  
pp. 778-792 ◽  
Author(s):  
Remco Koninckx ◽  
Karen Hensen ◽  
Annick Daniëls ◽  
Marjan Moreels ◽  
Ivo Lambrichts ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Neonatal Rat ◽  
Human Bone Marrow ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

scholarly journals High-affinity prorenin binding to cardiac man-6-P/IGF-II receptors precedes proteolytic activation to renin

2001 ◽  
Vol 280 (4) ◽  
pp. H1706-H1715 ◽  
Author(s):  
Jasper J. Saris ◽  
Frans H. M. Derkx ◽  
René J. A. De Bruin ◽  
Dick H. W. Dekkers ◽  
Jos M. J. Lamers ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Binding Sites ◽  
Neonatal Rat ◽  
Proteolytic Activation ◽  
Rat Cardiomyocytes ◽  
High Affinity ◽  
Neonatal Rat Cardiomyocytes ◽  
Factor Ii ◽  
Number Of Binding Sites ◽  
Kinetics Of

Mannose-6-phosphate (man-6-P)/insulin-like growth factor-II (man-6-P/IgF-II) receptors are involved in the activation of recombinant human prorenin by cardiomyocytes. To investigate the kinetics of this process, the nature of activation, the existence of other prorenin receptors, and binding of native prorenin, neonatal rat cardiomyocytes were incubated with recombinant, renal, or amniotic fluid prorenin with or without man-6-P. Intact and activated prorenin were measured in cell lysates with prosegment- and renin-specific antibodies, respectively. The dissociation constant ( Kd) and maximum number of binding sites (Bmax) for prorenin binding to man-6-P/IGF-II receptors were 0.6 ± 0.1 nM and 3,840 ± 510 receptors/myocyte, respectively. The capacity for prorenin internalization was greater than 10 times Bmax. Levels of internalized intact prorenin decreased rapidly (half-life = 5 ± 3 min) indicating proteolytic prosegment removal. Prorenin subdivision into man-6-P-free and man-6-P-containing fractions revealed that only the latter was bound. Cells also bound and activated renal but not amniotic fluid prorenin. We concluded that cardiomyocytes display high-affinity binding of renal but not extrarenal prorenin exclusively via man-6-P/IGF-II receptors. Binding precedes internalization and proteolytic activation to renin thereby supporting the concept of cardiac angiotensin formation by renal prorenin.

Oxidative stress enhances phosphorylation of p53 in neonatal rat cardiomyocytes

2007 ◽  
Vol 303 (1-2) ◽  
pp. 167-174 ◽  
Author(s):  
Xilin Long ◽  
Michael J. Goldenthal ◽  
José Marín-García
Keyword(s):  
Oxidative Stress ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes
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