Present and past climatic effects on the current distribution and genetic diversity of the Iberian spadefoot toad (Pelobates cultripes): an integrative approach

2016 ◽  
Vol 44 (2) ◽  
pp. 245-258 ◽  
Author(s):  
Jorge Gutiérrez-Rodríguez ◽  
A. Márcia Barbosa ◽  
Íñigo Martínez-Solano
Keyword(s):  
Genetic Diversity ◽  
Current Distribution ◽  
Integrative Approach ◽  
Climatic Effects ◽  
Spadefoot Toad ◽  
Pelobates Cultripes

Speciation of the genus Dolichopoda in Anatolia with reference to the role of ancient central lake system

2016 ◽  
Vol 47 (3) ◽  
pp. 267-283
Author(s):  
Mehmet Sait Taylan ◽  
Deniz Şirin
Keyword(s):  
Genetic Diversity ◽  
16S Rdna ◽  
Current Distribution ◽  
Phylogenetic Analyses ◽  
Evolutionary Relationships ◽  
Habitat Types ◽  
16S Rdna Gene ◽  
Lake System ◽  
Mitochondrial 16S Rdna

The aim of this study was to determine of the evolutionary relationships of the TurkishDolichopodaspecies, and to define and clarify the systematics of the group in Anatolia. For this purpose, we have examined some 27 populations of the genusDolichopodafrom Anatolia and adjacent regions (Caucasia and the Greek islands). A total of 532 bp of the mitochondrial 16S rDNA gene was sequenced in order to clarify the phylogenetic relationships of the genus. Five main clades can be distinguished and well-supported by phylogenetic analyses. In these clades, the results obtained from Samos Island and Anatolia are remarkable. In contrast to some previous studies, our results point to an Aegean origin of the AnatolianDolichopodaspecies. According to the mitochondrial 16S rDNA divergence data, the current distribution of the genetic diversity was affected by ancient central lake system, habitat types, Messinian period and Plio-Pleistocene characterized by alternating dry/cold and warm/humid stages.

scholarly journals A Snapshot of the Genetic Diversity of Salmonella Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1490
Author(s):  
Codruta-Romanita Usein ◽  
Mihaela Oprea ◽  
Adriana Simona Ciontea ◽  
Sorin Dinu ◽  
Daniela Cristea ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Salmonella Enteritidis ◽  
Tandem Repeats ◽  
High Throughput Sequencing ◽  
Minimum Spanning Tree ◽  
Public Health Practice ◽  
National Level ◽  
Variable Number ◽  
Integrative Approach ◽  
Sequencing Data

In the absence of consistent national molecular typing data to enhance the surveillance of Salmonella Enteritidis, it was considered useful to collect baseline information on the genetic diversity and antibiotic susceptibility of strains isolated in Romania between January 2016 and April 2020 and compare them to strains described in major international outbreaks of the same period. A collection of 245 clinical isolates were genotyped by a standardised multiple-locus variable-number of tandem repeats analysis (MLVA) 5-loci protocol and screened for antimicrobial resistance against 15 compounds. Twenty strains were further subjected to whole genome sequencing (WGS) and compared to epidemiologically relevant high-throughput sequencing data available in European databases. Twenty-seven MLVA genotypes were identified, of which three, commonly reported in Europe between 2016–2020, covered 72% of the collection. Antibiotic resistance was detected in 30% of the strains, with resistance to nalidixic acid and ciprofloxacin as the most common phenotype, and also associated with two prevalent MLVA clones. WGS-derived multilocus sequence typing (MLST) revealed a single sequence type (ST11) further resolved into 10 core-genome MLST complex types. The minimum spanning tree constructed from the cgMLST data clustered Romanian and international strains, which shared more than 95% of the core genes, revealing links with a contemporaneous multi-country outbreak. This study could be regarded as a forerunner to the advent of using this integrative approach in the public health practice at a national level and thus contribute to the concerted actions at a European level to stop outbreaks.

Mitochondrial diversity of Western spadefoot toads, Pelobates cultripes, in northwestern Spain

2010 ◽  
Vol 31 (3) ◽  
pp. 443-448 ◽  
Author(s):  
Angelica Crottini ◽  
Pedro Galán ◽  
Miguel Vences
Keyword(s):  
Dna Sequences ◽  
Phylogeographic Structure ◽  
Mitochondrial Cytochrome ◽  
Central Spain ◽  
Spadefoot Toads ◽  
Spadefoot Toad ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Northwestern Spain ◽  
Pelobates Cultripes

AbstractWe analysed the DNA sequences of the mitochondrial cytochrome b gene for 49 specimens of the Western spadefoot toad, Pelobates cultripes, from Spain, and one sample from France, with a focus on populations from Galicia in northwestern Spain. Our results point to a generally low mitochondrial differentiation of the species across its distributional range although some phylogeographic structure was detected. Galician populations from coastal localities, from the A Limia region, and from Monforte appeared to be closely related, whereas the only available sample from the relict Galician population from near O Barco de Valdeorras has the same haplotype as the samples from Burgos, in central Spain.

Development and characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad, Pelobates cultripes

2008 ◽  
Vol 10 (4) ◽  
pp. 993-996 ◽  
Author(s):  
Mirjam S. van de Vliet ◽  
Onno E. Diekmann ◽  
Ester T. A. Serrão ◽  
Pedro Beja
Keyword(s):  
Microsatellite Loci ◽  
Spadefoot Toad ◽  
Polymorphic Microsatellite ◽  
Pelobates Cultripes

The change in genetic diversity down the core-edge gradient in the eastern spadefoot toad (Pelobates syriacus)

2010 ◽  
Vol 19 (13) ◽  
pp. 2675-2689 ◽  
Author(s):  
INBAR MUNWES ◽  
ELI GEFFEN ◽  
URI ROLL ◽  
ADAM FRIEDMANN ◽  
ALON DAYA ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
The Core ◽  
Spadefoot Toad

scholarly journals Physiological Responses of the Submerged Macrophyte Stuckenia pectinata to High Salinity and Irradiance Stress to Assess Eutrophication Management and Climatic Effects: An Integrative Approach

Water ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1706
Author(s):  
Lamprini Malea ◽  
Konstantinia Nakou ◽  
Apostolos Papadimitriou ◽  
Athanasios Exadactylos ◽  
Sotiris Orfanidis
Keyword(s):  
High Salinity ◽  
Photosynthetic Pigment ◽  
High Irradiance ◽  
Growth Strategy ◽  
Submerged Macrophyte ◽  
Integrative Approach ◽  
Climatic Effects ◽  
Chl A ◽  
Stuckenia Pectinata ◽  
Acclimation Potential

Stuckenia pectinata, a submerged macrophyte of eutrophic to hyper-eutrophic fresh to brackish waters, faces management and climatic-forced increment of salinity and irradiance in Vistonis Lake (Greece) that may endanger its existence and the ecosystem functioning. A pre-acclimated clone under low irradiance and salinity conditions was treated to understand the effects of high salinity and irradiance on a suite of subcellular (chlorophyll a fluorescence kinetics and JIP-test, and chlorophyll content) to organismal (relative growth rate—RGR) physiological parameters. The responses to high irradiance indicated the plant’s great photo-acclimation potential to regulate the number and size of the reaction centers and the photosynthetic electron transport chain by dissipation of the excess energy to heat. A statistically significant interaction (p < 0.01) of salinity and irradiance on Chl a, b content indicated acclimation potential through adjusting the Chl a, b contents. However, no significant (p > 0.05) difference was observed on Chl a/b ratio and the RGR, indicating the species’ potential to become acclimatized by reallocating resources to compensate for growth. Thus, the regulation of photosynthetic pigment content and photosystem II performance consisted of the primary growth strategy to present and future high salinity and irradiance stressful conditions due to eutrophication management and the ongoing climatic changes.

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