Primary dermal fibroblasts and pectoralis muscle show similar patterns of oxidative stress in tropical and temperate birds despite differing life‐histories

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Ursula Konstantin Beattie ◽  
Haviland Wright ◽  
Ana Gabriela Jimenez
Keyword(s):  
Oxidative Stress ◽  
Life Histories ◽  
Dermal Fibroblasts ◽  
Pectoralis Muscle ◽  
Temperate Birds

scholarly journals Insights Into Antioxidant Activities and Anti-Skin-Aging Potential of Callus Extract From Centella Asiatica (L.)

2021 ◽  
Author(s):  
Visarut Buranasudja ◽  
Dolly Rani ◽  
Ashwini Malla ◽  
Khwanlada Kobtrakul ◽  
Sornkanok Vimolmangkang
Keyword(s):  
Oxidative Stress ◽  
Callus Culture ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Bioactive Metabolites ◽  
Chemical Components ◽  
Chemical Compositions ◽  
Scale Production

Abstract Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Results from TLC and HPLC analysis showed that callus extract has unique chemical components, compared with those isolated from authentic plant. Moreover, callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.

Lack of variation in nuclear DNA content in avian muscle

Genome ◽  
2021 ◽  
pp. 1-9
Author(s):  
Ana Gabriela Jimenez ◽  
Emily Gray Lencyk
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Muscle Size ◽  
Pectoralis Muscle ◽  
Long Distance ◽  
Cross Sectional ◽  
Avian Muscle ◽  
Myonuclear Domain ◽  
Temperate Birds

The avian pectoralis muscle demonstrates plasticity with regard to size, so that temperate birds facing winter conditions or birds enduring a migration bout tend to have significant increases in the size and mass of this tissue due to muscular hypertrophy. Myonuclear domain (MND), the volume of cytoplasm a myonuclei services, in the pectoralis muscle of birds seems to be altered during thermal stress or changing seasons. However, there is no information available regarding muscle DNA content or ploidy level within the avian pectoralis. Changes in muscle DNA content can be used in this tissue to aid in size and mass changes. Here, we hypothesized that long-distance migrants or temperate residents would use the process of endoreduplication to aid in altering muscle size. Mostly contradictory to our hypotheses, we found no differences in the mean muscle DNA content in any of the 62 species of birds examined in this study. We also found no correlations between mean muscle DNA content and other muscle structural measurements, such as the number of nuclei per millimeter of fiber, myonuclear domain, and fiber cross-sectional area. Thus, while avian muscle seems more phenotypically plastic than mammalian muscle, the biological processes surrounding myonuclear function may be more closely related to those seen in mammals.

scholarly journals Inonotus obliquus Extracts Decreased Expression of MMP1 mRNA via JNK-AP-1 Axis

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 36
Author(s):  
Young Joo Kim ◽  
Hwa Jun Cha
Keyword(s):  
Oxidative Stress ◽  
Matrix Metalloproteinases ◽  
Mrna Expression ◽  
Transcriptional Activity ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Inonotus Obliquus ◽  
The Family ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts

Inonotus obliquus, which is parasitic on birch and other trees, is a fungus in the family Hymenochaetaceae. In this study, we investigated whether Inonotus obliquus extracts used in traditional medicine were decreased in the expression of matrix metalloproteinases-1 (MMP-1) in the normal human dermal fibroblasts. As shown in our results, extracts of Inonotus obliquus decreased MMP1 expression in oxidative stress-exposed normal human dermal fibroblasts. Additionally, Inonotus obliquus extracts decreased AP-1 transcriptional activity and phospho-JNK in oxidative stress-exposed normal human dermal fibroblasts. Oxidative stress mediated the elevation of MMP1 mRNA expression and was well regulated by the JNK-AP-1 axis. Therefore, the results suggest that Inonotus obliquus extracts decreased MMP1 mRNA expression by regulating JNK-AP-1 axis. Additionally, Inonotus obliquus extracts have the potential to reduce collagen destruction and the formation of wrinkles and to be used as a cosmetic ingredient.

scholarly journals Protective Effect of the Aqueous Extract of Deschampsia antarctica (EDAFENCE®) on Skin Cells against Blue Light Emitted from Digital Devices

2020 ◽  
Vol 21 (3) ◽  
pp. 988 ◽  
Author(s):  
Silvia Lorrio ◽  
Azahara Rodríguez-Luna ◽  
Pablo Delgado-Wicke ◽  
Marta Mascaraque ◽  
María Gallego ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Blue Light ◽  
Aqueous Extract ◽  
Electronic Devices ◽  
High Energy ◽  
Dermal Fibroblasts ◽  
Light Irradiation ◽  
Deschampsia Antarctica ◽  
Pathway Activation

Skin is being increasingly exposed to artificial blue light due to the extensive use of electronic devices. This, together with recent observations reporting that blue light—also known as high-energy visible light—can exert cytotoxic effects associated with oxidative stress and promote hyperpigmentation, has sparked interest in blue light and its potential harmful effects on skin. The photoprotective properties of new extracts of different botanicals with antioxidant activity are therefore being studied. Deschampsia antarctica (Edafence®, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection effects against ultraviolet radiation and dioxin toxicity. In this regard, we studied the protective capacity of EDA against the deleterious effects of artificial blue light irradiation in human dermal fibroblasts (HDF) and melanocytes. We analyzed the impact of EDA on viability, cell morphology, oxidative stress, melanogenic signaling pathway activation and hyperpigmentation in HDF and melanocytes subjected to artificial blue light irradiation. Our results show that EDA protects against cell damage caused by artificial blue light, decreasing oxidative stress, melanogenic signaling pathway activation and hyperpigmentation caused by blue light irradiation. All these findings suggest that EDA might help prevent skin damage produced by artificial blue light exposure from screen of electronic devices.

Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress

2018 ◽  
Vol 73 (1) ◽  
pp. 18-24 ◽  
Author(s):  
José M. Alvarez-Suarez ◽  
Francesca Giampieri ◽  
Massimiliano Gasparrini ◽  
Luca Mazzoni ◽  
Tamara Y. Forbes-Hernández ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Crude Extract ◽  
Psidium Guajava ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals Antioxidant and Pro-Oxidant Properties of Carthamus Tinctorius, Hydroxy Safflor Yellow A, and Safflor Yellow A

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 119 ◽  
Author(s):  
Tiziana Bacchetti ◽  
Camilla Morresi ◽  
Luisa Bellachioma ◽  
Gianna Ferretti
Keyword(s):  
Oxidative Stress ◽  
Low Density Lipoprotein ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Carthamus Tinctorius ◽  
Dermal Fibroblasts ◽  
Water Soluble ◽  
Flower Extract ◽  
Bioactive Properties ◽  
Tert Butyl Hydroperoxide

(1) Carthamus Tinctorius L. (safflower) is extensively used in traditional herbal medicine. (2) The aim of this study was to investigate the bioactive properties of polyphenol extracts from flowers of Carthamus Tinctorius (CT) cultivated in Italy. We also evaluated the properties of two bioactive water-soluble flavonoid compounds, hydroxy safflor yellow A (HSYA) and safflor yellow A (SYA), contained in Carthamus Tinctorius petals. (3) The total polyphenol content was 3.5 ± 0.2 g gallic acid equivalent (GAE)/100 g, flavonoids content was 330 ± 23 mg catechin equivalent (CE)/100 g in the flowers. The extract showed a high antioxidant activity evaluated by oxygen radical absorbance capacity (ORAC) and 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assays. In addition, flower extract, SYA, and HSYA were able to reduce the susceptibility of low-density lipoprotein to copper-induced lipid peroxidation. In order to investigate the bioactive properties of flower extract, SYA, and HSYA we also studied their modulatory effect of oxidative stress on human dermal fibroblasts (HuDe) oxidized by tert-butyl hydroperoxide (t-BOOH). The CT extract at concentrations ranging from 0.01–20 μg GAE/mL of polyphenols, exerted a protective effect against t-BOOH triggered oxidative stress. At higher concentration the extract exerted a pro-oxidant effect. Similar results have been obtained using HSYA and SYA. (4) These results demonstrate a biphasic effect exerted by HSYA, SYA, and flower extracts on oxidative stress.

scholarly journals Andrographis paniculata and Its Bioactive Diterpenoids Protect Dermal Fibroblasts against Inflammation and Oxidative Stress

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 432 ◽  
Author(s):  
Eugenie Mussard ◽  
Sundy Jousselin ◽  
Annabelle Cesaro ◽  
Brigitte Legrain ◽  
Eric Lespessailles ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dermal Fibroblasts ◽  
Andrographis Paniculata ◽  
Type I ◽  
Ros Production ◽  
Skin Damage ◽  
Aging Effects ◽  
Natural Treatment ◽  
Tnf Α

Andrographis paniculata (Burm.f.) has long been used in ayurvedic medicine through its anti-inflammatory properties. However, its protective effect of skin aging has not been studied in vitro. This study aimed to investigate the anti-aging effects of methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12) on human dermal fibroblasts (HDFa) under pro-oxidant or pro-inflammatory condition. The in vitro anti-aging capacity of ME, ANDRO, NEO, 14DAP, and 14DAP11-12 (1, 2.5 and 5 µg/mL) was performed in HDFa. Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by the fluorescence of DCF-DA probe and cytokines were quantified by ELISA (IL6 and IL8) or RTqPCR (TNF-α). Procollagen type I production was determined by an ELISA. Our results showed a decrease in ROS production with ME and 14DAP at 5 µg/mL and 1 µg/mL, respectively. Furthermore, IL-6 production and TNF-α expression decreased under ANDRO and ME at 5 µg/mL. Our data indicated that ME and 14DAP protect from oxidative stress. Additionally, ME and ANDRO decreased an inflammation marker, IL-6. This suggests their potential natural treatment against skin damage. Hence, their applications could be of interest in cosmetics for preventing skin ageing.

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