scholarly journals Enterococcal biofilm – a nidus for antibiotic resistance transfer?

2022 ◽  
Author(s):  
M Conwell ◽  
JSG Dooley ◽  
PJ Naughton
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Transfer

Characterization of Cefotaxime- and Ciprofloxacin-Resistant CommensalEscherichia coliOriginating from Belgian Farm Animals Indicates High Antibiotic Resistance Transfer Rates

2018 ◽  
Vol 24 (6) ◽  
pp. 707-717 ◽  
Author(s):  
Ellen Lambrecht ◽  
Eva Van Meervenne ◽  
Nico Boon ◽  
Tom Van de Wiele ◽  
Pierre Wattiau ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Farm Animals ◽  
Transfer Rates ◽  
Resistance Transfer

scholarly journals Abstracts from the Biofilm Club meeting held at the School of Pharmacy, University of Manchester on 4 November 2004

Biofilms ◽  
2005 ◽  
Vol 2 (1) ◽  
pp. 73-78
Keyword(s):  
Antibiotic Resistance ◽  
Gastrointestinal Tract ◽  
Respiratory Mucosa ◽  
Resistance Transfer ◽  
Oral Biofilms ◽  
Club Meeting ◽  
University Of Manchester

Microfabrication of biofilmsModelling drain biofilmsInteraction between mycobacteria and the respiratory mucosaModelling the gastrointestinal tractModelling oral biofilmsModelling antibiotic resistance transfer in oral biofilmsPlaque biofilms grown in vivo on natural enamel

scholarly journals Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation

2019 ◽  
Vol 1 (1A) ◽  
Author(s):  
Mary McCarron ◽  
James Dooley ◽  
Ibrahim Banat ◽  
Joerg Arnscheidt ◽  
William Snelling
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Resistance Transfer

scholarly journals Metal ions as antibacterial agents

2018 ◽  
Vol 8 (6-s) ◽  
pp. 411-419 ◽  
Author(s):  
Sirisha Mittapally ◽  
Ruheena Taranum ◽  
Sumaiya Parveen
Keyword(s):  
Antibiotic Resistance ◽  
Environmental Protection Agency ◽  
Antibiotic Resistance Genes ◽  
Synergistic Activity ◽  
Transfer Factors ◽  
E Coli ◽  
Resistance Transfer ◽  
Low Toxicity ◽  
Arsenic Copper ◽  
R Factors

Metals like mercury, arsenic, copper and silver have been used in various forms as antimicrobials for thousands of years. The use of metals in treatment was mentioned in Ebers Papyrus (1500BC); i.e, copper to decrease inflammation & iron to overcome anemia. Copper has been registered at the U.S. Environmental Protection Agency as the earliest solid antimicrobial material. Copper is used for the treatment of different E. coli, MRSA, Pseudomonas infections. Advantage of use of silver is it has low toxicity to human’s cells than bacteria.It is less susceptible to gram +ve bacteria than gram –bacteria due to its thicker cell wall. Zinc is found to be active against Streptococcus pneumonia, Campylobacter jejuni. Silver & zinc act against vibrio cholera & enterotoxic E. coli. The use of metals as antibacterial got reduce with discovery of antibiotics in twentieth century, immediately after that antibiotic resistance was seen due to transfer of antibiotic resistance genes by plasmids also known as Resistance Transfer Factors or R-factors. Metal complexes are used to show synergistic activity against bacteria’s like copper & chlorhexidine on dental plaque bacteria, silver nanoparticles & cephalexin against E. coli & S. aureus. Keywords: Metals, Oligodynamic effect, Copper, Silver

scholarly journals In vitro evalution of antibiotic resistance of Lactobacillus bulgaricus strains isolated from traditional dairy products

2019 ◽  
Vol 37 (No. 1) ◽  
pp. 36-43
Author(s):  
Huiling Guo ◽  
Bilige Menghe
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Fermented Milk ◽  
Lactobacillus Bulgaricus ◽  
Dilution Method ◽  
Resistance Transfer ◽  
Traditional Dairy Products

The antimicrobial susceptibility of 20 Lb. bulgaricus isolates from traditional fermented milk-originated was assessed and then determined the ability to transfer antibiotic resistance genes to other bacteria. The minimum inhibitory concentration of each strain was determined using a standardized dilution method. All the tested strains were found to be susceptible to gentamicin, erythromycin, clindamycin, neomycin, tetracycline, linezolid, chloramphenicol, rifampicin, and quinupristin/dalfopristin, while their susceptibilities to kanamycin, ciprofloxacin, streptomycin, trimethoprim, ampicillin, and vancomycin varied. Polymerase chain reaction (PCR) was used to check whether specific antibiotic resistance genes were present in these Lb. bulgaricus. We detected the rpoB, erm(B), aadA, bla, cat and vanX. Finally, a filter mating assay was applied to investigate the transferability of these resistance markers; and we observe no antibiotic resistance transfer between bacteria. This work demonstrates a low risk of lateral transfer of the antibiotic resistance gene of Lb. bulgaricus.

Low Temperature and Modified Atmosphere: Hurdles for Antibiotic Resistance Transfer?

2015 ◽  
Vol 78 (12) ◽  
pp. 2191-2199 ◽  
Author(s):  
EVA VAN MEERVENNE ◽  
ELS VAN COILLIE ◽  
STEPHANIE VAN WEYENBERG ◽  
NICO BOON ◽  
LIEVE HERMAN ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Low Temperature ◽  
Modified Atmosphere Packaging ◽  
Plasmid Transfer ◽  
High Density ◽  
Modified Atmosphere ◽  
Low Density ◽  
Lactobacillus Sakei ◽  
Cooked Ham ◽  
Resistance Transfer

Food is an important dissemination route for antibiotic-resistant bacteria. Factors used during food production and preservation may contribute to the transfer of antibiotic resistance genes, but research on this subject is scarce. In this study, the effect of temperature (7 to 37°C) and modified atmosphere packaging (air, 50% CO2–50% N2, and 100% N2) on antibiotic resistance transfer from Lactobacillus sakei subsp. sakei to Listeria monocytogenes was evaluated. Filter mating was performed on nonselective agar plates with high-density inocula. A more realistic setup was created by performing modified atmosphere experiments on cooked ham using high-density and low-density inocula. Plasmid transfer was observed between 10 and 37°C, with plasmid transfer also observed at 7°C during a prolonged incubation period. When high-density inocula were used, transconjugants were detected, both on agar plates and cooked ham, under the three atmospheres (air, 50% CO2–50% N2, and 100% N2) at 7°C. This yielded a median transfer ratio (number of transconjugants/number of recipients) with an order of magnitude of 10−4 to 10−6. With low-density inocula, transfer was only detected under the 100% N2 atmosphere after 10-day incubation at 7°C, yielding a transfer ratio of 10−5. Under this condition, the highest bacterial density was obtained. The results indicate that low temperature and modified atmosphere packaging, two important hurdles in the food industry, do not necessarily prevent plasmid transfer from Lactobacillus sakei subsp. sakei to Listeria monocytogenes.

scholarly journals Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli

2001 ◽  
Vol 7 (4-5) ◽  
pp. 756-762
Author(s):  
A. Jafari ◽  
M. Katouli ◽  
F. Shokouhi ◽  
S. Bouzari
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
High Rate ◽  
Cell Adherence ◽  
Chain Reaction ◽  
Resistance Transfer ◽  
Polymerase Chain

The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates.

Effects of nano-TiO2on antibiotic resistance transfer mediated by RP4 plasmid

2015 ◽  
Vol 9 (7) ◽  
pp. 895-904 ◽  
Author(s):  
Zhigang Qiu ◽  
Zhiqiang Shen ◽  
Di Qian ◽  
Min Jin ◽  
Dong Yang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Transfer
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