Mutations associated with Helicobacter pylori antimicrobial resistance in the Ecuadorian population

2021 ◽  
Author(s):  
Jeannete Zurita ◽  
Gabriela Sevillano ◽  
Ariane Paz y Miño ◽  
Camilo Zurita ◽  
Vicente Peñaherrera ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antimicrobial Resistance

scholarly journals Genomic Analysis of Antimicrobial Resistance Genotype-to-Phenotype Agreement in Helicobacter pylori

2020 ◽  
Vol 9 (1) ◽  
pp. 2
Author(s):  
Tal Domanovich-Asor ◽  
Yair Motro ◽  
Boris Khalfin ◽  
Hillary A. Craddock ◽  
Avi Peretz ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antimicrobial Resistance ◽  
Point Mutations ◽  
Genomic Analysis ◽  
23S Rrna ◽  
Phenotype Correlation ◽  
Bioinformatics Pipeline ◽  
Phenotypic Resistance ◽  
Commercial Kits ◽  
H Pylori

Antimicrobial resistance (AMR) in Helicobacter pylori is increasing and can result in treatment failure and inappropriate antibiotic usage. This study used whole genome sequencing (WGS) to comprehensively analyze the H. pylori resistome and phylogeny in order to characterize Israeli H. pylori. Israeli H. pylori isolates (n = 48) underwent antimicrobial susceptibility testing (AST) against five antimicrobials and WGS analysis. Literature review identified 111 mutations reported to correlate with phenotypic resistance to these antimicrobials. Analysis was conducted via our in-house bioinformatics pipeline targeting point mutations in the relevant genes (pbp1A, 23S rRNA, gyrA, rdxA, frxA, and rpoB) in order to assess genotype-to-phenotype correlation. Resistance rates of study isolates were as follows: clarithromycin 54%, metronidazole 31%, amoxicillin 10%, rifampicin 4%, and levofloxacin 2%. Genotype-to-phenotype correlation was inconsistent; for every analyzed gene at least one phenotypically susceptible isolate was found to have a mutation previously associated with resistance. This was also observed regarding mutations commonly used in commercial kits to diagnose AMR in H. pylori cases. Furthermore, 11 novel point mutations associated with a resistant phenotype were detected. Analysis of a unique set of H. pylori isolates demonstrates that inferring resistance phenotypes from WGS in H. pylori remains challenging and should be optimized further.

scholarly journals Helicobacter pylori isolated from Iranian drinking water: vacA , cagA , iceA , oipA and babA2 genotype status and antimicrobial resistance properties

FEBS Open Bio ◽  
2016 ◽  
Vol 6 (5) ◽  
pp. 433-441 ◽  
Author(s):  
Reza Ranjbar ◽  
Faham Khamesipour ◽  
Nematollah Jonaidi‐Jafari ◽  
Ebrahim Rahimi
Keyword(s):  
Helicobacter Pylori ◽  
Drinking Water ◽  
Antimicrobial Resistance

Helicobacter pylori and antimicrobial resistance: molecular mechanisms and clinical implications

2006 ◽  
Vol 6 (11) ◽  
pp. 699-709 ◽  
Author(s):  
Monique M Gerrits ◽  
Arnoud HM van Vliet ◽  
Ernst J Kuipers ◽  
Johannes G Kusters
Keyword(s):  
Helicobacter Pylori ◽  
Antimicrobial Resistance ◽  
Molecular Mechanisms ◽  
Clinical Implications
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