Testing the efficacy of topical antimicrobial treatments using a two‐ and five‐species chronic wound biofilm model

2021 ◽  
Author(s):  
Andreea‐Gabriela Nedelea ◽  
Rebecca L. Plant ◽  
Lori I. Robins ◽  
Sarah E. Maddocks
Keyword(s):  
Chronic Wound ◽  
Biofilm Model ◽  
Antimicrobial Treatments ◽  
Topical Antimicrobial

In vitro multispecies Lubbock chronic wound biofilm model

2008 ◽  
Vol 16 (6) ◽  
pp. 805-813 ◽  
Author(s):  
Yan Sun ◽  
Scot E. Dowd ◽  
Ethan Smith ◽  
Dan D. Rhoads ◽  
Randall D. Wolcott
Keyword(s):  
Chronic Wound ◽  
Biofilm Model

scholarly journals MrkD1Pfrom Klebsiella pneumoniae Strain IA565 Allows for Coexistence with Pseudomonas aeruginosa and Protection from Protease-Mediated Biofilm Detachment

2013 ◽  
Vol 81 (11) ◽  
pp. 4112-4120 ◽  
Author(s):  
Brandon M. Childers ◽  
Tricia A. Van Laar ◽  
Tao You ◽  
Steven Clegg ◽  
Kai P. Leung
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Single Species ◽  
Wild Type ◽  
Content Type ◽  
Biofilm Model ◽  
Essential Components

ABSTRACTBiofilm formation and persistence are essential components for the continued survival of pathogens inside the host and constitute a major contributor to the development of chronic wounds with resistance to antimicrobial compounds. Understanding these processes is crucial for control of biofilm-mediated disease. Though chronic wound infections are often polymicrobial in nature, much of the research on chronic wound-related microbes has focused on single-species models.Klebsiella pneumoniaeandPseudomonas aeruginosaare microbes that are often found together in wound isolates and are able to form stablein vitrobiofilms, despite the antagonistic nature ofP. aeruginosawith other organisms. Mutants of theK. pneumoniaestrain IA565 lacking the plasmid-bornemrkD1Pgene were less competitive than the wild type in anin vitrodual-species biofilm model withP. aeruginosa(PAO1). PAO1 spent medium inhibited the formation of biofilm ofmrkD1P-deficient mutants and disrupted preestablished biofilms, with no effect on IA565 and no effect on the growth of the wild type or mutants. A screen using a two-allele PAO1 transposon library identified the LasB elastase as the secreted effector involved in biofilm disruption, and a purified version of the protein produced results similar to those with PAO1 spent medium. Various other proteases had a similar effect, suggesting that the disruption of themrkD1Pgene causes sensitivity to general proteolytic effects and indicating a role for MrkD1Pin protection against host antibiofilm effectors. Our results suggest that MrkD1Pallows for competition ofK. pneumoniaewithP. aeruginosain a mixed-species biofilm and provides defense against microbial and host-derived proteases.

Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model

2009 ◽  
Vol 18 (12) ◽  
pp. 508-512 ◽  
Author(s):  
S.E. Dowd ◽  
Y. Sun ◽  
E. Smith ◽  
J.P. Kennedy ◽  
C.E. Jones ◽  
...  
Keyword(s):  
Chronic Wound ◽  
Biofilm Model

scholarly journals A Model for Evaluating Topical Antimicrobial Efficacy against Methicillin-Resistant Staphylococcus aureus Biofilms in Superficial Murine Wounds

2012 ◽  
Vol 56 (8) ◽  
pp. 4508-4510 ◽  
Author(s):  
Eric D. Roche ◽  
Paul J. Renick ◽  
Shannon P. Tetens ◽  
Dennis L. Carson
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Infection Model ◽  
Superficial Infection ◽  
New Agents ◽  
Methicillin Resistant ◽  
Content Type ◽  
Biofilm Model ◽  
Topical Antimicrobial

ABSTRACTA wound biofilm model was created by adapting a superficial infection model. Partial-thickness murine wounds were inoculated with methicillin-resistantStaphylococcus aureus(MRSA). Dense biofilm communities developed at the wound surface after 24 h as demonstrated by microscopy and quantitative microbiology. Common topical antimicrobial agents had reduced efficacy when treatment was initiated 24 h after inoculation compared to 4 h after inoculation. This model provides a rapidin vivotest for new agents to treat wound biofilm infections.

scholarly journals Chronic Wound Biofilm Model

2015 ◽  
Vol 4 (7) ◽  
pp. 382-388 ◽  
Author(s):  
Kasturi Ganesh ◽  
Mithun Sinha ◽  
Shomita S. Mathew-Steiner ◽  
Amitava Das ◽  
Sashwati Roy ◽  
...  
Keyword(s):  
Chronic Wound ◽  
Biofilm Model

Effectiveness of Honey on S.aureus and P.aeruginosa Biofilms

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P107-P107 ◽  
Author(s):  
Talal Alandejani ◽  
Joseph G Marsan ◽  
Wendy Ferris ◽  
Slinger Robert ◽  
Frank Chan
Keyword(s):  
Bacterial Resistance ◽  
Bacterial Biofilms ◽  
Bacterial Isolates ◽  
Manuka Honey ◽  
Antibiotic Resistant ◽  
New Approach ◽  
Biofilm Model ◽  
Planktonic Form ◽  
And Staphylococcus Aureus ◽  
Topical Antimicrobial

Problem Biofilms formed by Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) have been shown to be an important factor in the pathophysiology of chronic rhinosinusitis (CRS). Growth in biofilms increases bacterial resistance to antibiotics, which may explain why CRS responds poorly to antibiotic therapy. Honey has been used as an effective topical antimicrobial agent in the treatment of antibiotic-resistant bacterial wound infections. To our knowledge, no research has studied the effect of honey on biofilms. Methods We used a previously established biofilm model to assess antibacterial activity of Manuka honey from New Zealand and Sidr honey from Yemen. These were tested at a 1 in 2 dilution against 11 isolates of methicillin-susceptible SA (MSSA), 11 methicillin-resistant SA (MRSA), and 11 PA isolates. Honeys were tested against both planktonic (broth) and biofilm-grown bacteria. Results Both honeys were effective in killing 100% of the isolates in the planktonic form. Biofilm-grown bacteria were less susceptible to the honeys, but honey still had significant bactericidal activity. The bactericidal rate for the Sidr and Manuka honeys against MSSA MRSA and PA were 63%, 73%, 91% and 82%, 63% and 91%, respectively. These rates were significantly higher (p<0.001) than those seen with single antibiotics commonly used against MSSA and MRSA (e.g. cloxacillin and vancomycin) in a previous study done on the same bacterial isolates. Conclusion Honey, which is a natural, non-toxic and inexpensive product, is effective in killing SA and PA bacterial biofilms. Significance This intriguing observation may have important clinical implications and could lead to a new approach for treating refractory CRS.

Propagation of anaerobic bacteria within an aerobic multi-species chronic wound biofilm model

2009 ◽  
Vol 18 (10) ◽  
pp. 426-431 ◽  
Author(s):  
Y. Sun ◽  
E. Smith ◽  
R. Wolcott ◽  
S.E. Dowd
Keyword(s):  
Chronic Wound ◽  
Anaerobic Bacteria ◽  
Biofilm Model
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