Correlation between exopolysaccharide biosynthesis and gastrointestinal tolerance of Lactiplantibacillus plantarum

2021 ◽  
Author(s):  
Qingqing Wu ◽  
Chenchen Zhang ◽  
Yunchao Wa ◽  
Hengxian Qu ◽  
Ruixia Gu ◽  
...  
Keyword(s):  
Gastrointestinal Tolerance ◽  
Exopolysaccharide Biosynthesis

Regulation of Exopolysaccharide Biosynthesis in Pseudomonas aeruginosa

2016 ◽  
pp. 171-189
Author(s):  
Yuta Okkotsu ◽  
Christopher L. Pritchett ◽  
Michael J. Schurr
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Exopolysaccharide Biosynthesis

Bioinformatic analysis of the Vibrio diabolicus genome for an exopolysaccharide biosynthesis locus

2012 ◽  
Author(s):  
V. Boursicot ◽  
G. LaPointe ◽  
C. Sinquin ◽  
J. Ratiskol ◽  
S. Colliec-Jouault ◽  
...  
Keyword(s):  
Bioinformatic Analysis ◽  
Exopolysaccharide Biosynthesis

scholarly journals Complex Enterally Tube-Fed Community Patients Display Stable Tolerance, Improved Compliance and Better Achieve Energy and Protein Targets with a High-Energy, High-Protein Peptide-Based Enteral Tube Feed: Results from a Multi-Centre Pilot Study

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3538
Author(s):  
Benjamin Green ◽  
Katy Sorensen ◽  
Mary Phillips ◽  
Lisa Green ◽  
Rachel Watson ◽  
...  
Keyword(s):  
Pilot Study ◽  
Protein Intake ◽  
Nutrient Intake ◽  
High Energy ◽  
High Protein ◽  
Medium Chain Triglycerides ◽  
Community Based ◽  
Protein Targets ◽  
Gastrointestinal Tolerance ◽  
Community Patients

This pilot study evaluated a high-energy, high-protein, peptide-based, (medium-chain triglycerides) MCT-containing enteral tube feed (Nutrison Peptisorb Plus HEHP®, Nutricia Ltd., Trowbridge, BA14 0XQ, UK.) containing 1.5 kcal/mL and 7.5 g protein/100 mL. Fifteen community-based, enterally tube-fed adults (42 (SD 16.3) years) received the intervention feed daily for 28 days, with gastrointestinal tolerance, compliance and nutrient intake assessed at baseline and after the intervention period. Incidence and intensity of constipation (p = 0.496), nausea (p = 1.000), abdominal pain (p = 0.366) and bloating (p = 0.250) remained statistically unchanged, yet the incidence and intensity of diarrhoea improved significantly after receiving the intervention feed (Z = −2.271, p = 0.023). Compliance with the intervention feed was significantly greater compared to the patient’s baseline regimens (99% vs. 87%, p = 0.038). Compared to baseline, use of the intervention feed enabled patients to significantly increase total energy (1676 kcal/day (SD 449) to 1884 kcal/day (SD 537), p = 0.039) and protein intake (73 g/day (SD 17) to 89 g/day (SD 23), p = 0.001), allowing patients to better achieve energy (from 88% to 99%, p = 0.038) and protein (from 101% to 121%, p < 0.001) requirements. This pilot study demonstrates that a high-energy, high-protein, peptide-based, MCT-containing enteral tube feed maintains gastrointestinal tolerance and improves compliance, energy and protein intake in complex, enterally tube-fed, community-based adult patients, though more work is recommended to confirm this.

scholarly journals A Diverse Repertoire of Exopolysaccharide Biosynthesis Gene Clusters in Lactobacillus Revealed by Comparative Analysis in 106 Sequenced Genomes

2019 ◽  
Vol 7 (10) ◽  
pp. 444 ◽  
Author(s):  
Dipti Deo ◽  
Dimple Davray ◽  
Ram Kulkarni
Keyword(s):  
Biofilm Formation ◽  
Gene Clusters ◽  
High Variability ◽  
Protein Families ◽  
Free Living ◽  
Biosynthesis Gene ◽  
Exopolysaccharide Biosynthesis ◽  
Pharmaceutical Industries ◽  
Living Group ◽  
Core Genes

Production of exopolysaccharides (EPS) is one of the unique features of Lactobacillus genus. EPS not only have many physiological roles such as in stress tolerance, quorum sensing and biofilm formation, but also have numerous applications in the food and pharmaceutical industries. In this study, we identified and compared EPS biosynthesis gene clusters in 106 sequenced Lactobacillus genomes representing 27 species. Of the 146 identified clusters, only 41 showed the typical generic organization of genes as reported earlier. Hierarchical clustering showed highly varied nature of the clusters in terms of the gene composition; nonetheless, habitat-wise grouping was observed for the gene clusters from host-adapted and nomadic strains. Of the core genes required for EPS biosynthesis, epsA, B, C, D and E showed higher conservation, whereas gt, wzx and wzy showed high variability in terms of the number and composition of the protein families. Analysis of the distribution pattern of the protein families indicated a higher proportion of mutually exclusive families in clusters from host-adapted and nomadic strains, whereas those from the free-living group had very few unique families. Taken together, this analysis highlights high variability in the EPS gene clusters amongst Lactobacillus with some of their properties correlated to the habitats.

The Streptococcus thermophilus protein Wzh functions as a phosphotyrosine phosphatase

2013 ◽  
Vol 59 (6) ◽  
pp. 391-398 ◽  
Author(s):  
Angela D. Cefalo ◽  
Jeffery R. Broadbent ◽  
Dennis L. Welker
Keyword(s):  
Metal Binding ◽  
Protein Tyrosine Kinase ◽  
Streptococcus Thermophilus ◽  
Amino Acid Residues ◽  
Sodium Vanadate ◽  
Phosphotyrosine Phosphatase ◽  
Sequence Comparisons ◽  
Exopolysaccharide Biosynthesis ◽  
Phosphotyrosine Phosphatases ◽  
Phosphate Groups

Amino acid residues that are important for metal binding and catalysis in Gram-positive phosphotyrosine phosphatases were identified in the Wzh protein of Streptococcus thermophilus MR-1C by using sequence comparisons. A His-tagged fusion Wzh protein was purified from Escherichia coli cultures and tested for phosphatase activity against synthetic phosphotyrosine and phosphoserine–threonine peptides. Purified Wzh released 2316.5 ± 138.7 pmol PO4·min−1·μg−1 from phosphotyrosine peptide-1 and 2345.7 ± 135.2 pmol PO4·min−1·μg−1 from phosphotyrosine peptide-2. The presence of the phosphotyrosine phosphatase inhibitor sodium vanadate decreased purified Wzh activity by 45%–50% at 1 mmol·L–1, 74%–84% at 5 mmol·L–1, and by at least 88% at 10 mmol·L–1. Purified Wzh had no detectable activity against the phosphoserine–threonine peptide. These results clearly establish that S. thermophilus MR-1C Wzh functions as a phosphotyrosine phosphatase that could function to remove phosphate groups from proteins involved in exopolysaccharide biosynthesis, including the protein tyrosine kinase Wze and priming glycosyltransferase.

Sign in / Sign up

Export Citation Format

Share Document