scholarly journals Aspect ratio of nano/microstructures determines Staphylococcus aureus adhesion on PET and titanium surfaces

2021 ◽  
Author(s):  
Ann‐Kathrin Meinshausen ◽  
Maria Herbster ◽  
Christoph Zwahr ◽  
Marcos Soldera ◽  
Andreas Müller ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Aspect Ratio ◽  
Titanium Surfaces

PolyNaSS grafting on titanium surfaces enhances osteoblast differentiation and inhibits Staphylococcus aureus adhesion

2013 ◽  
Vol 24 (7) ◽  
pp. 1745-1754 ◽  
Author(s):  
A. Alcheikh ◽  
G. Pavon-Djavid ◽  
G. Helary ◽  
H. Petite ◽  
V. Migonney ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Osteoblast Differentiation ◽  
Titanium Surfaces

Self-organised nanoarchitecture of titanium surfaces influences the attachment of Staphylococcus aureus and Pseudomonas aeruginosa bacteria

2015 ◽  
Vol 99 (16) ◽  
pp. 6831-6840 ◽  
Author(s):  
Vi Khanh Truong ◽  
Vy T. H. Pham ◽  
Alexander Medvedev ◽  
Rimma Lapovok ◽  
Yuri Estrin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Titanium Surfaces

Nanorough titanium surfaces reduce adhesion of Escherichia coli and Staphylococcus aureus via nano adhesion points

2016 ◽  
Vol 145 ◽  
pp. 617-625 ◽  
Author(s):  
Claudia Lüdecke ◽  
Martin Roth ◽  
Wenqi Yu ◽  
Uwe Horn ◽  
Jörg Bossert ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Titanium Surfaces ◽  
And Staphylococcus Aureus

Rubidium-doped titanium surfaces with modulatory effects on MC3T3-E1 cell response and antibacterial capacity against Staphylococcus aureus

2019 ◽  
Vol 14 (4) ◽  
pp. 045016 ◽  
Author(s):  
Manke Chen ◽  
Shusong Wu ◽  
Yanni Tan ◽  
Runcheng Li ◽  
Yong Liu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Response ◽  
Titanium Surfaces ◽  
Antibacterial Capacity

Single High Aspect Ratio Pillar Support Structures: Multi-Scale Chip Integrated Conformal Structures

2004 ◽  
Author(s):  
M. T. Northen ◽  
K. L. Turner
Keyword(s):  
Aspect Ratio ◽  
Silicon Dioxide ◽  
High Aspect Ratio ◽  
Single Crystal Silicon ◽  
Fiber Surface ◽  
Processing Technique ◽  
Crystal Silicon ◽  
Nano Fiber ◽  
Titanium Surfaces ◽  
Conformal Structures

This paper describes the realization of the integration of micro and nanoscale conformal structures. A new dry processing technique is used to create structures supported by a single high aspect ratio pillar support (SHARPS), creating submicron single crystal silicon features, with tight geometric control, attached to much larger (10–200μm) silicon dioxide platforms. The platforms have lithographically defined two dimensional shapes. The curvature of the platforms is adjusted through metal deposition and subsequent processing. The flexibility of the structures allows them to conform to meso and microscale roughness. To account for nanoscale conformance stochastic arrays of vertically aligned titania nanofibers are created on the platform surface. The application of the system as a passive microadhesive is investigated using a nanoindenter. Results show that the titania nano fiber surface offers significant increase in adhesion over smooth silicon dioxide or titanium surfaces. However when used in conjunction with the SHARPS structures the fibers are too stiff to allow for conformance to the surface prior to larger scale conformation, thus the combination does not increase the overall adhesion.

scholarly journals Antibacterial Activity against Staphylococcus Aureus of Titanium Surfaces Coated with Graphene Nanoplatelets to Prevent Peri-Implant Diseases. An In-Vitro Pilot Study

2020 ◽  
Vol 17 (5) ◽  
pp. 1568 ◽  
Author(s):  
Nicola Pranno ◽  
Gerardo La Monaca ◽  
Antonella Polimeni ◽  
Maria Sabrina Sarto ◽  
Daniela Uccelletti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pilot Study ◽  
Dental Implants ◽  
Graphene Nanoplatelets ◽  
Titanium Implants ◽  
Titanium Surfaces ◽  
Biofilm Development ◽  
Titanium Disk

Dental implants are one of the most commonly used ways to replace missing teeth. Nevertheless, the close contact with hard and soft oral tissues expose these devices to infectious peri-implant diseases. To prevent such infection, several surface treatments have been developed in the last few years to improve the antimicrobial properties of titanium dental implants. In this in-vitro pilot study, the antimicrobial activity of titanium surfaces coated with different types of graphene nanoplatelets are investigated. Six different colloidal suspensions of graphene nanoplatelets (GNPs) were produced from graphite intercalated compounds, setting the temperature and duration of the thermal shock and varying the number of the exfoliation cycles. Titanium disks with sand-blasted and acid-etched surfaces were sprayed with 2 mL of colloidal GNPs suspensions. The size of the GNPs and the percentage of titanium disk surfaces coated by GNPs were evaluated through a field emission-scanning electron microscope. The antibacterial activity of the specimens against Staphylococcus aureus was estimated using a crystal violet assay. The dimension of GNPs decreased progressively after each sonication cycle. The two best mean percentages of titanium disk surfaces coated by GNPs were GNPs1050°/2 and GNPs1150°/2. The reduction of biofilm development was 14.4% in GNPs1150°/2, 20.1% in GNPs1150°/3, 30.3% in GNPs1050°/3, and 39.2% in GNPs1050°/2. The results of the study suggested that the surface treatment of titanium disks with GNPs represents a promising solution to improve the antibacterial activity of titanium implants.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

Attachment of oral bacteria to titanium surfaces: An SEM study

1994 ◽  
Vol 52 ◽  
pp. 468-469
Author(s):  
J. E. Laffoon ◽  
R. L. Anderson ◽  
J. C. Keller ◽  
C. D. Wu-Yuan
Keyword(s):  
Technical Problem ◽  
Titanium Implant ◽  
Oral Bacteria ◽  
Bacterial Cells ◽  
Thin Sections ◽  
Surface Ultrastructure ◽  
Sem Study ◽  
Key Factor ◽  
Titanium Surfaces

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).

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