scholarly journals Capture of a novel, antibiotic resistance encoding, mobile genetic element from Escherichia coli using a new entrapment vector

2020 ◽  
Author(s):  
S. Tansirichaiya ◽  
S.J. Moyo ◽  
M. Al‐Haroni ◽  
A.P. Roberts
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Mobile Genetic Element ◽  
Genetic Element

scholarly journals IS1397 Is Active for Transposition into the Chromosome of Escherichia coli K-12 and Inserts Specifically into Palindromic Units of Bacterial Interspersed Mosaic Elements

1999 ◽  
Vol 181 (22) ◽  
pp. 6929-6936 ◽  
Author(s):  
Jean-Marie Clément ◽  
Caroline Wilde ◽  
Sophie Bachellier ◽  
Patricia Lambert ◽  
Maurice Hofnung
Keyword(s):  
Escherichia Coli ◽  
Mobile Genetic Element ◽  
Basic Element ◽  
Genetic Element ◽  
Repeated Element ◽  
E Coli ◽  
Intergenic Regions ◽  
Natural Isolates ◽  
K 12

ABSTRACT We demonstrate that IS1397, a putative mobile genetic element discovered in natural isolates of Escherichia coli, is active for transposition into the chromosome of E. coliK-12 and inserts specifically into palindromic units, also called repetitive extragenic palindromes, the basic element of bacterial interspersed mosaic elements (BIMEs), which are found in intergenic regions of enterobacteria closely related to E. coli andSalmonella. We could not detect transposition onto a plasmid carrying BIMEs. This unprecedented specificity of insertion into a well-characterized chromosomal intergenic repeated element and its evolutionary implications are discussed.

scholarly journals Structure of the Capsid Size-Determining Scaffold of “Satellite” Bacteriophage P4

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 953
Author(s):  
James L. Kizziah ◽  
Cynthia M. Rodenburg ◽  
Terje Dokland
Keyword(s):  
Escherichia Coli ◽  
Life Cycle ◽  
Mobile Genetic Element ◽  
Gene Products ◽  
Genetic Element ◽  
Size Change ◽  
Capsid Formation ◽  
High Resolution Structure ◽  
Bacteriophage P4 ◽  
Resolution Structure

P4 is a mobile genetic element (MGE) that can exist as a plasmid or integrated into its Escherichia coli host genome, but becomes packaged into phage particles by a helper bacteriophage, such as P2. P4 is the original example of what we have termed “molecular piracy”, the process by which one MGE usurps the life cycle of another for its own propagation. The P2 helper provides most of the structural gene products for assembly of the P4 virion. However, when P4 is mobilized by P2, the resulting capsids are smaller than those normally formed by P2 alone. The P4-encoded protein responsible for this size change is called Sid, which forms an external scaffolding cage around the P4 procapsids. We have determined the high-resolution structure of P4 procapsids, allowing us to build an atomic model for Sid as well as the gpN capsid protein. Sixty copies of Sid form an intertwined dodecahedral cage around the T = 4 procapsid, making contact with only one out of the four symmetrically non-equivalent copies of gpN. Our structure provides a basis for understanding the sir mutants in gpN that prevent small capsid formation, as well as the nms “super-sid” mutations that counteract the effect of the sir mutations, and suggests a model for capsid size redirection by Sid.

scholarly journals Aquaculture changes the profile of antibiotic resistance and mobile genetic element associated genes in Baltic Sea sediments

2016 ◽  
Vol 92 (4) ◽  
pp. fiw052 ◽  
Author(s):  
Windi I. Muziasari ◽  
Katariina Pärnänen ◽  
Timothy A. Johnson ◽  
Christina Lyra ◽  
Antti Karkman ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Baltic Sea ◽  
Mobile Genetic Element ◽  
Genetic Element

scholarly journals Whole-genome sequence analysis reveals evolution of antimicrobial resistance in a Ugandan colistin resistant Acinetobacter baumannii

2020 ◽  
Author(s):  
Dickson Aruhomukama ◽  
Ivan Sserwadda ◽  
Gerald Mboowa
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Mobile Genetic Element ◽  
Whole Genome Sequence ◽  
Broad Host Range ◽  
Insertion Sequences ◽  
Drug Resistant ◽  
Genetic Element ◽  
Resistance Evolution

AbstractIn recent times, pan-drug resistant Acinetobacter baumannii have emerged and continue to spread among critically ill patients, this poses an urgent risk to global and local human health. This study sought to provide the first genomic analysis of a pan-drug resistant Acinetobacter baumannii from Uganda and Africa, and to tell a story of mobile genetic element-mediated antibiotic resistance evolution in the isolate. It was an in-silico study in which intrinsic and acquired antibiotic resistance genes, and/or chromosomal resistance mutations were identified using PATRIC, CARD, NDARO and ResFinder. Screening for insertion sequences was done using ISfinder. Also, plasmid screening, phylogenetic analysis and sequence typing were performed using PlasmidFinder, PATRIC and Gubbin, and MLST respectively.The isolate belonged to the Sequence type 136, belonging to Clonal complex 208 and Global complex 2. This isolate shared close homology with strains from Tanzania. Resistance in the isolate was chromosomally and mobile genetic element-mediated by Acinetobacter-derived cephalosporinases and carbapenem hydrolyzing class D β-lactamses, blaOXA-2, 51, 5 88, 317, blaADC-2, 25. Colistin resistance was associated with previously documented mutants, lpxA and lpxC. Other key resistance genes identified were: aph(3”)-lb, aph(6)-ld, aph(3’)-la, aac(3)-lld, aac(3)-lla, aph(3’)-l, aph(3”)-l, aph(6)-lc, aph(6)-ld, aac(3)-II, III, IV, VI, VIII, IX, X, macA, macB, tetA, tetB, tetR, dfrA, and those of the floR family. RSF1010 like IncQ broad-host-range plasmids and features of pACICU1, pACICU2, and p3ABAYE Acinetobacter baumannii plasmids namely partitioning proteins ParA and B were present. Insertion sequences present included IS3, IS5, IS66 and those of the ISLre2 families.The study described for the first time a pan-drug resistant Acinetobacter baumannii from Uganda, and told a story of mobile genetic element-mediated antibiotic resistance evolution in the isolate despite being limited by pan-drug resistance phenotypic data. It provides a basis to track trends in antibiotic resistance and identification of emerging resistance patterns in Acinetobacter baumannii in Uganda.

scholarly journals The role of antibiotic resistance mobile genetic element MCR-1 in enhancing bacterial survival in macrophages

2021 ◽  
Vol 2022 (1) ◽  
Author(s):  
Amina Hashim ◽  
Mahmoud Elgamal ◽  
Shazeda H. Chowdhury ◽  
Fatima A. Hattab ◽  
Rafal Al-Shibly ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Mobile Genetic Element ◽  
Bacterial Survival ◽  
Genetic Element

Antibiotic-Resistant Enterococcus faecalis in Abattoir Pigs and Plasmid Colocalization and Cotransfer of tet(M) and erm(B) Genes

2012 ◽  
Vol 75 (9) ◽  
pp. 1595-1602 ◽  
Author(s):  
CINDY-LOVE TREMBLAY ◽  
ANN LETELLIER ◽  
SYLVAIN QUESSY ◽  
DANIELLE DAIGNAULT ◽  
MARIE ARCHAMBAULT
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Mobile Genetic Element ◽  
Antibiotic Resistance Genes ◽  
Genetic Element ◽  
Antibiotic Resistant ◽  
Resistance Determinants ◽  
Physical Linkage ◽  
Genes Encoding ◽  
Plasmid Profiling

This study was conducted to determine plasmid colocalization and transferability of both erm(B) and tet(M) genes in Enterococcus faecalis isolates from abattoir pigs in Canada. A total of 124 E. faecalis isolates from cecal contents of abattoir pigs were examined for antibiotic susceptibility. High percentages of resistance to macrolides and tetracyclines were found. Two predominant multiresistance patterns of E. faecalis were examined by PCR and sequencing for the presence of genes encoding antibiotic resistance. Various combinations of antibiotic resistance genes were detected; erm(B) and tet(M) were the most common genes. Plasmid profiling and hybridization revealed that both genes were colocated on a ~9-kb transferable plasmid in six strains with the two predominant multiresistant patterns. Plasmid colocalization and cotransfer of tet(M) and erm(B) genes in porcine E. faecalis isolates indicates that antibiotic coselection and transferability could occur via this single genetic element. To our knowledge, this is the first report on plasmid colocalization and transferability of erm(B) and tet(M) genes in E. faecalis on a mobile genetic element of ~9 kb. Physical linkage between important antibiotic resistance determinants in enterococci is of interest for predicting potential transfer to other bacterial genera.

scholarly journals Recombination between chromosomal IS200 elements supports frequent duplication formation in Salmonella typhimurium.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1245-1252 ◽  
Author(s):  
K R Haack ◽  
J R Roth
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Mobile Genetic Element ◽  
Repetitive Sequences ◽  
Mobile Genetic Elements ◽  
Genomic Variation ◽  
Chromosome Rearrangements ◽  
Genetic Element ◽  
High Frequencies ◽  
Typhimurium Strain

Abstract Spontaneous tandem chromosomal duplications are common in populations of Escherichia coli and Salmonella typhimurium. They range in frequency for a given locus from 10(-2) to 10(-4) and probably form by RecA-dependent unequal sister strand exchanges between repetitive sequences in direct order. Certain duplications have been observed previously to confer a growth advantage under specific selective conditions. Tandem chromosomal duplications are unstable and are lost at high frequencies, representing a readily reversible source of genomic variation. Six copies of a small mobile genetic element IS200 are evenly distributed around the chromosome of S. typhimurium strain LT2. A survey of 120 independent chromosomal duplications (20 for each of six loci) revealed that recombination between IS200 elements accounted for the majority of the duplications isolated for three of the loci tested. Duplications of the his operon were almost exclusively due to recombination between repeated IS200 elements. These data add further support to the idea that mobile genetic elements provide sequence repeats that play an important role in recombinational chromosome rearrangements, which may contribute to adaptation of bacteria to stressful conditions.

Sign in / Sign up

Export Citation Format

Share Document