Production of β‐galactosidase by Trichoderma sp. through solid‐state fermentation targeting the recovery of galactooligosaccharides from whey cheese

Author(s):  
L.F.M.C. Jesus ◽  
L.H.S. Guimarães
2010 ◽  
Vol 19 (5) ◽  
pp. 1387-1390 ◽  
Author(s):  
Juan Buenrostro-Figueroa ◽  
Heliodoro de la Garza-Toledo ◽  
Vrani Ibarra-Junquera ◽  
Cristóbal Noe Aguilar

Author(s):  
Norsalwani, T. T. L. ◽  
Nik Norulaini, N. A. R. ◽  
Nurul, J. H. ◽  
Massaud, M. B. N. ◽  
Nagao, H. ◽  
...  

2002 ◽  
Vol 57 (7-8) ◽  
pp. 666-670 ◽  
Author(s):  
Adriana B. Dartora ◽  
Telma E. Bertolin ◽  
Denise Bilibio ◽  
Mauricio M. Silveira ◽  
Jorge A. V. Costa

Five strains of filamentous fungi (Aspergillus niger strains NRRL 3122 and T0005007-2, Aspergillus oryzae CCT 3940, Aspergillus awamori NRRL 3112 and a Trichoderma sp.) were compared for their capacity to produce endo-polygalacturonase (endo-PG) in solid state fermentation. Maximum pectinolytic activity was reached in 72 h of growth, the best two fungal strains being A. niger T0005007-2 and A. oryzae CCT 3940. Three types of commercial purified pectin and four of unprocessed pectin (tangerine, orange, Tahiti lime and sweet lime rind) were used to assess the effect of pectin on the production of endo-PG by A. niger T0005007-2. Maximum pectinolytic activity was achieved using 6 and 10% (w/w) of purified pectin as inducer. Depending on the origin of the commercial pectin used as inducer, maximum endo-PG levels varied from 223 to 876 units per gram of dry medium (one endo-PG unit (U) was defined as the quantity of enzyme which caused a reduction in viscosity of 50% in a 1% w/v solution of pectin in 30 min), indicating that care should be taken when choosing this component of the medium. When the crude pectins were used as inducers at the same concentration as purified pectin, maximum endo-PG activities were 250-300 units/g. However, by increasing the amount of Tahiti lime rind to 50% (w/w) maximum endo-PG was 919 U/g, thus opening up the possibility of a low cost medium for endo-PG production.


Author(s):  
Diana NEAGU ◽  
Jacqueline DESTAIN ◽  
Phillipe THONART ◽  
Carmen SOCACIU

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher. 


Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
G Juodeikiene ◽  
D Cizeikiene ◽  
A Maruška ◽  
E Bartkiene ◽  
L Basinskiene ◽  
...  

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