Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

2020 ◽  
Author(s):  
L.X. Fu ◽  
J.S. Gong ◽  
B. Gao ◽  
D.J. Ji ◽  
X.G. Han ◽  
...  
Keyword(s):  
Lysis Gene

scholarly journals Mutational analysis of bacteriophage lambda lysis gene S.

1986 ◽  
Vol 167 (3) ◽  
pp. 1035-1042 ◽  
Author(s):  
R Raab ◽  
G Neal ◽  
J Garrett ◽  
R Grimaila ◽  
R Fusselman ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Bacteriophage Lambda ◽  
Lysis Gene

scholarly journals Amount of Colicin Release in Escherichia coli Is Regulated by Lysis Gene Expression of the Colicin E2 Operon

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119124 ◽  
Author(s):  
Andreas Mader ◽  
Benedikt von Bronk ◽  
Benedikt Ewald ◽  
Sara Kesel ◽  
Karin Schnetz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Lysis Gene ◽  
Colicin E2

Bacteriophage λ Lysis Gene Product Modified and Inserted intoEscherichia coliOuter Membrane: Rz1 Lipoprotein

1996 ◽  
Vol 2 (1) ◽  
pp. 147-153 ◽  
Author(s):  
ALINA TAYLOR ◽  
SABINA KEDZIERSKA ◽  
ALICJA WAWRZYNÓW
Keyword(s):  
Gene Product ◽  
Bacteriophage Λ ◽  
Lysis Gene

scholarly journals A cryptic lysis gene near the start of the Qβ replicase gene in the +1 frame

2004 ◽  
Vol 9 (10) ◽  
pp. 877-889 ◽  
Author(s):  
Tohru Nishihara ◽  
Hirokazu Morisawa ◽  
Norihito Ohta ◽  
John F. Atkins ◽  
Yukinobu Nishimura
Keyword(s):  
Replicase Gene ◽  
Lysis Gene

scholarly journals Identification of the Burkholderia pseudomallei bacteriophage ST79 lysis gene cassette

2016 ◽  
Vol 121 (2) ◽  
pp. 364-372 ◽  
Author(s):  
N. Khakhum ◽  
U. Yordpratum ◽  
A. Boonmee ◽  
U. Tattawasart ◽  
J.L.M. Rodrigues ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Gene Cassette ◽  
Lysis Gene

Lysis gene of bacteriophage MS2 is activated by translation termination at the overlapping coat gene

1987 ◽  
Vol 195 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Ben Berkhout ◽  
Brian F. Schmidt ◽  
Anja van Strien ◽  
Jacques van Boom ◽  
Jeroen van Westrenen ◽  
...  
Keyword(s):  
Translation Termination ◽  
Bacteriophage Ms2 ◽  
Lysis Gene

Use of the lysis gene of bacteriophage φX174 for the construction of a positive selection vector

Gene ◽  
1986 ◽  
Vol 42 (3) ◽  
pp. 345-349 ◽  
Author(s):  
Bernhard Henrich ◽  
Roland Plapp
Keyword(s):  
Positive Selection ◽  
Lysis Gene

scholarly journals Development and optimization of Lysis gene E as a counter-selection marker with high selection stringency

2021 ◽  
Author(s):  
Wei Chen ◽  
Ruyi Chen ◽  
Ling He ◽  
Xiaotong Wu
Keyword(s):  
Regulatory System ◽  
Selection Marker ◽  
Marker Genes ◽  
Selection System ◽  
Gram Negative ◽  
E Coli ◽  
Lysis Gene ◽  
Counter Selection ◽  
Practical Research ◽  
High Selection

Seamless modification of bacteria chromosome is widely performed both in theoretical and in practical research, for this purpose, excellent counter-selection marker genes with high selection stringency are needed. Lysis gene E from bacteriophage PhiX174 was developed and optimized as a counter-selection marker in this paper. Lysis gene E was firstly constructed under the control of pL promoter. At 42 °C, Lysis gene E could effectively kill Escherichia coli. Seamless modification using E as a counter-selection marker also successfully conducted. It also works in another Gram-negative strain Serratia marcescens under the control of Arac/PBAD regulatory system. Through combining lysis gene E and kil, the selection stringency frequency of pL-kil-sd-E cassette in E. coli arrived at 4.9×10−8 and 3.2×10−8 at two test loci, which is very close to the best counter-selection system, inducible toxins system. Under the control of Arac/PBAD, selection stringency of PBAD-kil-sd-E in S. marcescens arrived at the level of 10−7 at four test loci. By introducing araC gene harboring plasmid pKDsg-ack, 5- to 18- fold improvement of selection stringency was observed at these loci, and a surprising low selection stringency frequency 4.9×10−9 was obtained at marR-1 locus, the lowest selection stringency frequency for counter-selection reported so far. Similarly, at araB locus of E. coli selection stringency frequency of PBAD-kil-sd-E was improved to 3×10−9 after introducing plasmid pKDsg-ack. In conclusion, we have developed and optimized a newly universal counter-selection marker based on lysis gene E. The best selection stringency of this new marker exceeds the inducible toxins system several fold.

scholarly journals Conservation of a dual-start motif in P22 lysis gene regulation.

1990 ◽  
Vol 172 (1) ◽  
pp. 204-211 ◽  
Author(s):  
K Nam ◽  
U Bläsi ◽  
M T Zagotta ◽  
R Young
Keyword(s):  
Gene Regulation ◽  
Lysis Gene
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