Comparison of chemical extraction methods for determination of 146S content in foot‐and‐mouth disease oil‐adjuvanted vaccine

2019 ◽  
Vol 128 (1) ◽  
pp. 65-73
Author(s):  
P. Saravanan ◽  
Z. Iqbal ◽  
D.P.R. Selvaraj ◽  
M. Aparna ◽  
V. Umapathi ◽  
...  
2006 ◽  
Vol 4 (13) ◽  
pp. 235-241 ◽  
Author(s):  
Nicholas J Savill ◽  
Darren J Shaw ◽  
Rob Deardon ◽  
Michael J Tildesley ◽  
Matthew J Keeling ◽  
...  

Most of the mathematical models that were developed to study the UK 2001 foot-and-mouth disease epidemic assumed that the infectiousness of infected premises was constant over their infectious periods. However, there is some controversy over whether this assumption is appropriate. Uncertainty about which farm infected which in 2001 means that the only method to determine if there were trends in farm infectiousness is the fitting of mechanistic mathematical models to the epidemic data. The parameter values that are estimated using this technique, however, may be influenced by missing and inaccurate data. In particular to the UK 2001 epidemic, this includes unreported infectives, inaccurate farm infection dates and unknown farm latent periods. Here, we show that such data degradation prevents successful determination of trends in farm infectiousness.


Author(s):  
Veli Gülyaz ◽  
Fahriye Saraç ◽  
Can Çokçalışkan ◽  
Esra Satır ◽  
Beyhan Sareyyüpoğlu ◽  
...  

The sedimentation constant of the infective particle in foot-and-mouth disease has been determined by modifications of the methods of Elford (1936) and Polsen (1941). A new high-speed, swinging-cup rotor was employed. A sedimentation constant of 70 Svedberg units was obtained for the infective particle in a variety of starting materials derived from guineapigs, mice and cattle. The validity of the data is discussed in relation to the accuracy with which a sedimentation constant may be determined by these methods. Ultracentrifugal studies employing inclined tubes have demonstrated that in fresh preparations the infective particle is associated with from 0 to 50% of the initial complement-fixing activity. The remaining complement-fixing activity is associated with a component of sedimentation constant 8 Svedberg units. This slower sedimenting component, if infective, contributes less than 0⋅01% of the initial infectivity. A direct and relatively precise method is described for the determination of the partition of a biological activity between two or more components of a virus system. By the use of radial and inclined tubes in non-optical procedures a correlation between these methods has been established. It is shown that the sedimentation constant of a biologically active component may be estimated by procedures based on sampling in inclined tubes. The G integral is introduced as an accurate and convenient parameter which greatly facilitates the calculation and presentation of the results of ultracentrifugal studies.


2008 ◽  
Vol 20 (5) ◽  
pp. 598-605 ◽  
Author(s):  
Recai Tunca ◽  
Mahmut Sozmen ◽  
Hidayet Erdogan ◽  
Mehmet Citil ◽  
Erdogan Uzlu ◽  
...  

2008 ◽  
Vol 32 (2) ◽  
pp. 181-203
Author(s):  
Zaid Salah Hussein

For the determination of FMD infection in Nainawa province Serumsamples were collected from 92 suspected cases (42sheep,50cattle)thiswas performed in September 2007 and included nine region(Talafar,Basheca, Al-shamal, Hamamalaleel ,Cokajlly ,Zamaar ,Al-Namrood,AL-Koosh and Hemedaat), these samples were send frozen to the labs ofstate Vet. Company in Al-Nahdaa , the special test FMD-3ABC bo Ovenzyme immune assay on sheep serums showed that 30.95% werepositive to the test and 19.05% were ambiguous while the test on bovineserums showed increase in both positive and ambiguous results(42%,26% respectively), 8 serums that showed the highest positive resultto the former test were submitted to the special immunological test FMDElisa Kit Liquid Phase Blocking Immunoassay for detection of antibodiesof foot and mouth disease virus test which revealed that all sores of thesamples were infected with O serotype FMD


2017 ◽  
Vol 50 (12) ◽  
pp. 1420-1427 ◽  
Author(s):  
V. Zebec ◽  
D. Rastija ◽  
Z. Lončarić ◽  
A. Bensa ◽  
B. Popović ◽  
...  

2014 ◽  
Vol 30 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Yiyang DONG ◽  
Yan XU ◽  
Zaixin LIU ◽  
Yuanfang FU ◽  
Toshinori OHASHI ◽  
...  

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