scholarly journals In vitro inhibitory activity of probiotic products against oral Candida species

2016 ◽  
Vol 121 (1) ◽  
pp. 254-262 ◽  
Author(s):  
C. Zhao ◽  
X. Lv ◽  
J. Fu ◽  
C. He ◽  
H. Hua ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Candida Species ◽  
Oral Candida

Inhibitory activity in vitro of probiotic lactobacilli against oral Candida under different fermentation conditions

2015 ◽  
Vol 6 (3) ◽  
pp. 361-368 ◽  
Author(s):  
Q. Jiang ◽  
I. Stamatova ◽  
K. Kari ◽  
J.H. Meurman
Keyword(s):  
Inhibitory Activity ◽  
Candida Species ◽  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Lactobacillus Bulgaricus ◽  
Ph Values ◽  
Probiotic Lactobacilli ◽  
Oral Candida

Clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. Our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral Candida species. Sugar utilisation by both lactobacilli and Candida was also assessed. Agar overlay assay was utilised to study growth inhibition of Candida albicans, Candida glabrata and Candida krusei by Lactobacillus rhamnosus GG, Lactobacillus casei Shirota, Lactobacillus reuteri SD2112, Lactobacillus brevis CD2, Lactobacillus bulgaricus LB86 and L. bulgaricus LB Lact. The inhibitory effect was measured at pH 5.5, 6.4, and 7.2, respectively, and in the presence of five different carbohydrates in growth medium (glucose, fructose, lactose, sucrose, and sorbitol). Growth and final pH values were measured at two-hour time points to 24 h. L. rhamnosus GG showed the strongest inhibitory activity in fructose and glucose medium against C. albicans, followed by L. casei Shirota, L. reuteri SD2112 and L. brevis CD2. None of the lactobacilli tested affected the growth of C. krusei. Only L. rhamnosus GG produced slight inhibitory effect on C. glabrata. The lower pH values led to larger inhibition zones. Sugar fermentation profiles varied between the strains. L. casei Shirota grew in the presence of all sugars tested, whereas L. brevis CD2 could utilise only glucose and fructose. All Candida species metabolised the available sugars but the most rapid growth was observed with C. glabrata. The results suggest that commercially available probiotics differ in their inhibitory activity and carbohydrate utilisation; the above properties are modified by different pH values and sugars with more pronounced inhibition at lower pH.

scholarly journals Efficacy of tea tree, sesame, and cumin extracts against oral Candida species – An in Vitro study

2021 ◽  
Vol 33 (4) ◽  
pp. 357
Author(s):  
Keerthana Selvam ◽  
SumanJ Lakshmi ◽  
Elangovan Somasundaram ◽  
SenthilKumar Balasubramanian ◽  
Bakiyalakshmi Balasubramanian
Keyword(s):  
Candida Species ◽  
In Vitro Study ◽  
Vitro Study ◽  
Tea Tree ◽  
Oral Candida

scholarly journals Antifungal effect of green tea extracts on oral Candida species: An in vitro study

2016 ◽  
Vol 3 ◽  
pp. 1-4 ◽  
Author(s):  
M. G. Madhura ◽  
R. D. Shweta ◽  
B. Veerendra Kumar ◽  
D. Savithri ◽  
S. Gajalakshmi ◽  
...  
Keyword(s):  
Green Tea ◽  
Candida Species ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antifungal Effect ◽  
Oral Candida

scholarly journals Probiotic Lactobacillus reuteri has antifungal effects on oral Candida species in vitro

2017 ◽  
Vol 9 (1) ◽  
pp. 1274582 ◽  
Author(s):  
Mette Rose Jørgensen ◽  
Camilla Kragelund ◽  
Peter Østrup Jensen ◽  
Mette Kirstine Keller ◽  
Svante Twetman
Keyword(s):  
Candida Species ◽  
Lactobacillus Reuteri ◽  
Probiotic Lactobacillus ◽  
Antifungal Effects ◽  
Oral Candida

In vitro antifungal effect of amine fluoride-stannous fluoride combination on oral Candida species

Oral Diseases ◽  
2006 ◽  
Vol 12 (1) ◽  
pp. 45-50 ◽  
Author(s):  
JH Meurman ◽  
K Kari ◽  
T Waltimo ◽  
A Kotiranta ◽  
J Inkeri ◽  
...  
Keyword(s):  
Candida Species ◽  
Antifungal Effect ◽  
Stannous Fluoride ◽  
Amine Fluoride ◽  
Oral Candida

scholarly journals Oral Candida colonization in HIV-infected patients in Londrina-PR, Brazil: antifungal susceptibility and virulence factors

2015 ◽  
Vol 9 (12) ◽  
pp. 1350-1359 ◽  
Author(s):  
Suelen Balero De Paula ◽  
Alexandre Tadachi Morey ◽  
Jussevania Pereira Santos ◽  
Pollyanna M. C. Dos Santos ◽  
Danielle G. Gameiro ◽  
...  
Keyword(s):  
Candida Species ◽  
Biofilm Formation ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Cell Surface Hydrophobicity ◽  
Candida Colonization ◽  
Hydrophobic Property ◽  
Predisposing Factor ◽  
Oral Candida

Introduction: Host colonization by Candida species is an important predisposing factor to candidiasis, which seems to be more frequent in human immunodeficiency virus (HIV)-infected patients. Knowledge about the distribution, antifungal susceptibility, and virulence of oral Candida isolates is important for effective management of candidiasis. Methodology: Oral rinses were collected from 242 HIV-infected patients without clinical evidence of candidiasis seen at the AIDS referral center in Londrina, Brazil. Species were identified by standard phenotypic and molecular methods, and characterized in vitro according to antifungal susceptibility, cell surface hydrophobicity, biofilm formation, and enzyme activities. Results: Oral Candida colonization was detected in 50.4% of patients and combined use of antiretroviral therapy and protease inhibitor had a protective effect against colonization. Candida albicans (75.2%) was the most prevalent species. A high proportion of Candida spp. (39.9%) showed decreased susceptibility to fluconazole. Five isolates were resistant to nystatin. Protease and phospholipase activities were detected in 100% and 36.8% of isolates, respectively. Most isolates displayed a hydrophobic property that was associated with biofilm formation ability. Conclusions: A significant number of oral Candida species exhibiting decreased susceptibility to fluconazole were isolated from colonized HIV-infected individuals. Furthermore, all isolates expressed potential virulence attributes in vitro. Given the high incidence and severity of fungal infections in HIV-infected individuals, the results of this study reinforce the importance of antifungal susceptibility testing, which contributes to therapeutic strategies and highlights the need for continuous surveillance of Candida colonization in this population.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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