scholarly journals 3-Amino-4-aminoximidofurazan derivatives: small molecules possessing antimicrobial and antibiofilm activity against Staphylococcus aureus and Pseudomonas aeruginosa

2016 ◽  
Vol 120 (4) ◽  
pp. 842-859 ◽  
Author(s):  
M.C. Das ◽  
S. Paul ◽  
P. Gupta ◽  
P. Tribedi ◽  
S. Sarkar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Small Molecules ◽  
Antibiofilm Activity

scholarly journals Antibacterial and Antibiofilm Potential of Sea Anemone (Stichodactyla haddoni)-Isolated Vibrio (V. parahaemolyticus and V. alginolyticus), and Pseudoalteromonas (P. gelatinilytica and P. piscicida) Against Staphylococcus aureus and Pseudomonas aeruginosa

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Neda Fazeli ◽  
Akram Sadat Naeemi ◽  
Seyed Amir Hossein Jalali ◽  
Hojjatollah Zamani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Sea Anemone ◽  
Broth Culture ◽  
Antibiofilm Activity ◽  
Microtiter Plates ◽  
Protease Enzyme ◽  
Molecular Identification Methods ◽  
Stichodactyla Haddoni

Background: Staphylococcus aureus and Pseudomonas aeruginosa are important human bacterial pathogens, which are resistant to several antibiotics. One of the main causes of their resistance is the ability of biofilm formation. Objectives: The present study aimed to evaluate the antibacterial and antibiofilm activity of the extracts of Vibrio parahaemolyticus, V. alginolyticus, Pseudoalteromonas gelatinilytica, and Pseudoalteromonas piscicida isolated from sea anemone (Stichodactyla haddoni) against S. aureus and P. aeruginosa. Methods: Four isolated bacteria were identified using biochemical and molecular identification methods, and their extracts were obtained by mixing the cell-free supernatants from their old broth culture using ethyl acetate and methanol as the solvents. The agar well-diffusion and micro-dilution methods were also applied to determine the antibacterial activity, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) of the extracts. The ability of the extracts to inhibit biofilm formation and disrupt the preformed biofilm of the pathogens was attained through crystal violet staining in 96-well microtiter plates. To determine the nature of the extracts, they were exposed to protease enzyme, and the antibiofilm activity was compared with the untreated extracts. Results: The extracts of the four isolated bacteria inhibited bacterial growth and biofilm formation and disrupted the preformed biofilm of S. aureus (MIC = BIC = 600 µg/mL) and P. aeruginosa (MIC = BIC = 300 µg/mL). In addition, the active compounds of the extracts with antibiofilm activities were mainly proteases. Conclusions: According to the results, V. parahaemolyticus, V. alginolyticus, P. gelatinilytica, and P. piscicida had antibacterial and antibiofilm potential against S. aureus and P. aeruginosa, and their extract could also be further analyzed as an alternative to antibiotics.

scholarly journals Reply to Kumari and Singh, “Antibiofilm Activity of Small Molecules Produced by Staphylococcus epidermidis against Staphylococcus aureus”

2020 ◽  
Vol 86 (13) ◽  
Author(s):  
Thaís Glatthardt ◽  
Juliana Curityba de Mello Campos ◽  
Raiane Cardoso Chamon ◽  
Thiago Freitas de Sá Coimbra ◽  
Giulia de Almeida Rocha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Small Molecules ◽  
Staphylococcus Epidermidis ◽  
Antibiofilm Activity

scholarly journals Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5024
Author(s):  
Francesca Guzzo ◽  
Monica Scognamiglio ◽  
Antonio Fiorentino ◽  
Elisabetta Buommino ◽  
Brigida D’Abrosca
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Natural Products ◽  
Molecular Mechanisms ◽  
Antimicrobial Agents ◽  
Antibiofilm Activity ◽  
Complex Structures ◽  
Skin Infections ◽  
Human Pathogens ◽  
And Staphylococcus Aureus

Bacteria are social organisms able to build complex structures, such as biofilms, that are highly organized surface-associated communities of microorganisms, encased within a self- produced extracellular matrix. Biofilm is commonly associated with many health problems since its formation increases resistance to antibiotics and antimicrobial agents, as in the case of Pseudomonas aeruginosa and Staphylococcus aureus, two human pathogens causing major concern. P. aeruginosa is responsible for severe nosocomial infections, the most frequent of which is ventilator-associated pneumonia, while S. aureus causes several problems, like skin infections, septic arthritis, and endocarditis, to name just a few. Literature data suggest that natural products from plants, bacteria, fungi, and marine organisms have proven to be effective as anti-biofilm agents, inhibiting the formation of the polymer matrix, suppressing cell adhesion and attachment, and decreasing the virulence factors’ production, thereby blocking the quorum sensing network. Here, we focus on plant derived chemicals, and provide an updated literature review on the anti-biofilm properties of terpenes, flavonoids, alkaloids, and phenolic compounds. Moreover, whenever information is available, we also report the mechanisms of action.

scholarly journals Screening And Quantification Of Anti-Quorum Sensing And Antibiofilm Activities Of Phyllosphere Bacteria Against Biofilm Forming Bacteria

2019 ◽  
Author(s):  
NADINE AMABEL THEODORA ◽  
VANIA DOMINIKA ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Salmonella Typhimurium ◽  
Enterococcus Faecalis ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibiofilm Activity ◽  
Crude Extracts ◽  
Phyllosphere Bacteria

Abstract Objective The objectives of this research were to screen anti-quorum sensing activity of phyllosphere bacteria and quantify their antibiofilm activity against biofilm forming bacteria ( Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium, Vibrio cholerae, Pseudomonas aeruginosa). Results We found 11 phyllosphere bacteria isolates with potential anti-quorum sensing activity. Most of the crude extracts from phyllosphere bacteria isolates had anti-quorum sensing activity against Chromobacterium violaceum at certain concentration (20 and 10 mg/mL), but not crude extract from isolate JB 7F. Crude extracts showed various antibiofilm activities against all tested pathogenic bacteria.

Antibacterial and antibiofilm activity of the bacteriocin-producing strain Leuconostoc mesenteroides CHBY46 isolated from Algerian dromedary milk against Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2019 ◽  
Vol 8 (4) ◽  
pp. 120-131
Author(s):  
Yahia Bellil ◽  
Zineb Benmechernene ◽  
Wassila Chahrour Bellil ◽  
Mebrouk Kihal
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
16S Rrna Gene Sequencing ◽  
Antibiofilm Activity ◽  
Rrna Gene ◽  
Biofilm Inhibition ◽  
Staph Aureus ◽  
Spoilage Bacteria ◽  
Dromedary Milk ◽  
And Staphylococcus Aureus

In order to control biofilm formation of pathogenic and spoilage bacteria in foods, some species of Leuconostoc are very important in food industries, as they increase the shelf life of foods during preservation. In this study the strain CHBY46 a bacteriocin-producing strain belonging to Leuconostoc ge-nus isolated from dromedary milk in the south of Algeria was characterized by 16S rRNA gene sequencing and MALDITOF MS mass spectrometry, tested for its antibacterial and antibiofilm activities against Pseudomonas aeruginosa and Staphylococcus aureus. The produced bacteriocin was partially puri-fied with sulfate ammonium precipitation and RP-HPLC. The strain CHBY46 was classified as Leuc. mesenteroides after molecular identification. Among the bacteria tested the pathogens Staph. aureus ATCC 29213 and Ps. aeru-ginosa ATCC 27653 were sensitive to this bacteriocin with 480 AU/ml. Antibi-ofilm activity was investigated by crystal violet assay. The bacteriocin of Leuc. mesenteroides CHBY 46 exhibited significant biofilm inhibition ; 35.58% with Ps. aeruginosa, and 42.11% with Staph. aureus. Tricine-SDS-PAGE analysis of the partially purified bacteriocin indicated a low molecular weight of approximately 3.5 kDa. Therfore, we conclude that bacteriocins from Leuco-nostoc have the potential as a therapeutic strategy against pathogen’s bio-films, which contribute, to bacterial pathogenicity and resistance toward antibiotics or being used in foods as adjunsts to contribute food safety.

scholarly journals Effect of Bacoside A on growth and biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa

2017 ◽  
Vol 63 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Debaprasad Parai ◽  
Ekramul Islam ◽  
Jayati Mitra ◽  
Samir Kumar Mukherjee
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cell Membrane ◽  
Extracellular Polymeric Substances ◽  
Inhibitory Concentration ◽  
Membrane Integrity ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Bacoside A ◽  
Microscopic Studies

The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A were tested using the minimum inhibitory concentration and minimum bactericidal concentration assays. A cell membrane disruption assay was performed to find its possible target site. MTT assay, crystal violet assay, and microscopic studies were performed to assess the antibiofilm activity. Bacoside A showed antimicrobial activity against both test organisms in their planktonic and biofilm states. At a subminimum inhibitory concentration of 200 μg·mL−1, Bacoside A significantly removed ∼88%–93% of bacterial biofilm developed on microtiter plates. Biochemical and microscopic studies suggested that the eradication of biofilm might be due to the loss of extracellular polymeric substances and to a change in cell membrane integrity of the selected bacterial strains treated with Bacoside A. These results indicate that Bacoside A might be considered as an antimicrobial having the ability to disrupt biofilms. Thus, either alone or in combination with other therapeutics, Bacoside A could be useful to treat biofilm-related infections caused by opportunistic bacterial pathogens.

scholarly journals Silver Oxynitrate, an Unexplored Silver Compound with Antimicrobial and Antibiofilm Activity

2015 ◽  
Vol 59 (7) ◽  
pp. 4031-4039 ◽  
Author(s):  
Joe A. Lemire ◽  
Lindsay Kalan ◽  
Alexandru Bradu ◽  
Raymond J. Turner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiofilm Activity ◽  
Metal Compounds ◽  
Antibiotic Resistant ◽  
Content Type ◽  
New Antibiotics ◽  
Silver Compound ◽  
Therapeutic Compound

ABSTRACTHistorically it has been accepted, and recent research has established, that silver (Ag) is an efficacious antimicrobial agent. A dwindling pipeline of new antibiotics, combined with an increase in the number of antibiotic-resistant infections, is bringing Ag to the fore as a therapeutic compound to treat infectious diseases. Currently, many formulations of Ag are being deployed for commercial and medical purposes, with various degrees of effectiveness at killing microbial cells. Here, we evaluated the antimicrobial and antibiofilm capacity of our lead compound, silver oxynitrate [Ag(Ag3O4)2NO3or Ag7NO11], against other metal compounds with documented antimicrobial activity, including Ag2SO4, AgNO3, silver sulfadiazine (AgSD), AgO, Ag2O, and CuSO4. Our findings reveal that Ag7NO11eradicates biofilm and planktonic populations ofPseudomonas aeruginosa,Escherichia coli,Staphylococcus aureus, uropathogenicEscherichia coli(UPEC), fluoroquinolone-resistantPseudomonas aeruginosa(FQRP), and methicillin-resistantStaphylococcus aureus(MRSA) at lower concentrations than those of the other tested metal salts. Altogether, our results demonstrate that Ag7NO11has an enhanced efficacy for the treatment of biofilm-forming pathogens.

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