scholarly journals Development of a specific immunomagnetic capture‐ PCR for rapid detection of viable M ycoplasma agalactiae in sheep milk samples

2014 ◽  
Vol 117 (6) ◽  
pp. 1585-1591 ◽  
Author(s):  
G. Sanna ◽  
V. Lecca ◽  
A. Foddai ◽  
S. Tola
Keyword(s):  
Rapid Detection ◽  
Milk Samples ◽  
Sheep Milk ◽  
Immunomagnetic Capture

scholarly journals Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk

2014 ◽  
Vol 83 (10) ◽  
pp. S3-S8 ◽  
Author(s):  
Lenka Necidová ◽  
Šárka Bursová ◽  
Alena Skočková ◽  
Bohdana Janštová ◽  
Pavla Prachařová ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Storage Temperature ◽  
Goat Milk ◽  
Storage Conditions ◽  
Cow Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plate Method ◽  
Milk Samples ◽  
Sheep Milk ◽  
Enterotoxin Production

The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.

Validation of strip tests for the rapid screening of ethanol residues in milk

2019 ◽  
Vol 86 (4) ◽  
pp. 464-466
Author(s):  
Rafael Fagnani ◽  
Fernanda Montanholi de Lira ◽  
Carla Prado Rosolem
Keyword(s):  
Breast Milk ◽  
Rapid Detection ◽  
Final Diagnosis ◽  
Rapid Screening ◽  
Lower Sensitivity ◽  
Official Method ◽  
Good Sensitivity ◽  
Research Communication ◽  
Milk Samples ◽  
Strip Test

AbstractThe experiments reported in this Research Communication aimed to determine if a domestic strip test to detect ethanol residues in breast milk (Milkscreen®) is comparable to a previously established official method for detecting ethanol residues in milk. The two methods are examined in terms of overall sensitivity, robustness against storage and acidity and selectivity against formaldehyde residues. Here, Milkscreen® provided advantages, with faster results (2 min), good sensitivity (≥0.017%), no false results due formaldehyde residues and equal robustness against storage, but with lower sensitivity in acid milk samples. In summary, strip tests for the rapid detection of ethanol residues in breast milk can be used for screening purposes by dairy manufacturers, combining it with the official method to make a final diagnosis.

Rapid Detection of Psychrotrophic Bacteria In Manufacturing Grade Raw Milks

1991 ◽  
Vol 54 (11) ◽  
pp. 861-867 ◽  
Author(s):  
S. R. TATINI ◽  
P. MEKALA ◽  
A. EL-HABAZ ◽  
M. W. GRIFFITHS
Keyword(s):  
Rapid Detection ◽  
Bacterial Count ◽  
Raw Milk ◽  
Plate Count ◽  
Psychrotrophic Bacteria ◽  
Milk Samples ◽  
Microscopic Count ◽  
Direct Epifluorescent Filter Technique ◽  
Native Microflora

Methods to rapidly assess the bacteriological quality of raw milk were investigated. Whereas direct microscopic count, modified psychrotrophic plate count, and direct epifluorescent filter technique (DEFT) did not correlate well with initial psychrotrophic bacterial count of raw milk, improvements were obtained after preincubation of the milk samples. The best preincubation conditions were identified as 30°C for 6 h, 21°C for 10 h, 13°C for 15 h, 13°C for 20 h, or 7°C for 37 h. The “square root” equation was applied to the data, and a model was produced for predicting growth of the native microflora of raw milk. Using this equation, a DEFT count after preincubation of the milk at 21°C for 10 h could accurately predict the initial psychrotroph count and the count after storage of the milk at 6°C for 48 h.

scholarly journals Genotypic Characterization of Coagulase-negative Staphylococci Isolated from Sheep Milk in Slovakia

2010 ◽  
Vol 79 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Ivana Pilipčincová ◽  
Mangesh Bhide ◽  
Eva Dudriková ◽  
Milan Trávniček
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Biochemical Characterization ◽  
Pulse Field ◽  
Coagulase Negative Staphylococci ◽  
Pulse Field Gel Electrophoresis ◽  
Milk Samples ◽  
Sheep Milk ◽  
Epidemiological Situation

Hitherto very few reports are available presenting identification and molecular characterization of the coagulase negative staphylococci (CNS) from sheep milk in the subclinical stage of mastitis. Furthermore, very scanty data are available on the epidemiological status of CNS in different Slovak provinces. Milk samples from 54 sheep farms located in eastern Slovak region were screened. A total 240 CNS were identified with series of biochemical testes (STAPH-API) and subjected further for genotyping with the help of pulse field gel electrophoresis (PFGE). The most frequently occurring CNS species according the biochemical characterization were:S. epidermidis(36.3 %),S. caprae(21.3 %),S. hominis(6.6 %),S. chromogenes(6.3 %),S. xylosus(5.8 %),S. warneri(5.0 %) andS. capitis(4.6 %). Further PFGE-based characterization of these isolates revealed six pulsotypes of theS. epidermidis, two ofS. caprae, three ofS. chromogenes, nine ofS. hominis, five ofS. capitisand seven ofS. xylosus. These results contribute to knowledge of the epidemiological situation of the CNS from the subclinical form of mastitis in Slovakia.

Molecular detection of Brucella spp in goat and sheep milk samples from apparently healthy and infected animals

2017 ◽  
Vol 27 (3) ◽  
pp. 571-575
Author(s):  
Zahra Shirazi ◽  
Mohammad Khalili ◽  
Balal Sadeghi ◽  
Hamid Sharifi
Keyword(s):  
Molecular Detection ◽  
Milk Samples ◽  
Sheep Milk ◽  
Apparently Healthy

Effect of season on microbial and chemical characteristics of milk and yoghurt from sheep

2014 ◽  
Vol 54 (8) ◽  
pp. 1091 ◽  
Author(s):  
Razzagh Mahmoudi ◽  
Reza Norian ◽  
Mohammad Reza Pajohi-Alamoti ◽  
Javad Aliakbarlu
Keyword(s):  
Crude Protein ◽  
Titratable Acidity ◽  
Fat Content ◽  
Chemical Characteristics ◽  
Protein Nitrogen ◽  
Milk Samples ◽  
Sheep Milk ◽  
Casein Protein ◽  
Nitrogen Contents

The quality and hygienic status of milk are of importance for both dairy industries and consumers. In the present study, the effect of season on the quality and microbial properties of sheep milk and yoghurt produced from sheep milk were surveyed. In total, 30 sheep-milk samples were collected from January to August 2011, and yoghurt was produced from these samples. The fat content, titratable acidity and pH showed changes across the year. Seasonal variation was found to have a significant (P < 0.05) effect on nitrogen components, particularly crude protein, non-casein protein and non-protein nitrogen contents, fat content, pH and titratable acidity. Sensory analysis showed that the quality of yoghurt samples produced in winter was higher than that of yoghurt produced in summer (P < 0.05). This work showed the effect of season on chemical and sensory properties of sheep milk and yoghurt in Qazvin, Iran.

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