scholarly journals Effect of thermal treatment on free-living amoeba inactivation

2013 ◽  
Vol 116 (3) ◽  
pp. 728-736 ◽  
Author(s):  
S. Cervero-Aragó ◽  
S. Rodríguez-Martínez ◽  
O. Canals ◽  
H. Salvadó ◽  
R. M. Araujo
Keyword(s):  
Thermal Treatment ◽  
Free Living ◽  
Free Living Amoeba

Balamuthia mandrillaris: A Rare Free-Living Amoeba

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Jessica Chan ◽  
Mirella Mircescu ◽  
Pratik Shah ◽  
Andrew Liguori ◽  
Aaron Shmookler
Keyword(s):  
Free Living ◽  
Balamuthia Mandrillaris ◽  
Free Living Amoeba

A fatal case of meningoencephalitis due to a free-living amoeba of uncertain identity—probably acanthamoeba SP

Pathology ◽  
1981 ◽  
Vol 13 (1) ◽  
pp. 51-68 ◽  
Author(s):  
Rodney F. Carter ◽  
G.J. Cullity ◽  
V.J. Ojeda ◽  
P. Silberstein ◽  
E. Willaert
Keyword(s):  
Fatal Case ◽  
Free Living ◽  
Free Living Amoeba

Brain-eating Amoebae Infection: Challenges and Opportunities in Chemotherapy

2019 ◽  
Vol 19 (12) ◽  
pp. 980-987 ◽  
Author(s):  
Mohammad Ridwane Mungroo ◽  
Ayaz Anwar ◽  
Naveed Ahmed Khan ◽  
Ruqaiyyah Siddiqui
Keyword(s):  
Mortality Rate ◽  
Free Living ◽  
Outdoor Activities ◽  
The Past ◽  
Free Living Amoeba ◽  
Challenges And Opportunities ◽  
Life Threatening ◽  
The Central Nervous System ◽  
Living Nature ◽  
Treatment And Diagnosis

Pathogenic free-living amoeba are known to cause a devastating infection of the central nervous system and are often referred to as “brain-eating amoebae”. The mortality rate of more than 90% and free-living nature of these amoebae is a cause for concern. It is distressing that the mortality rate has remained the same over the past few decades, highlighting the lack of interest by the pharmaceutical industry. With the threat of global warming and increased outdoor activities of public, there is a need for renewed interest in identifying potential anti-amoebic compounds for successful prognosis. Here, we discuss the available chemotherapeutic options and opportunities for potential strategies in the treatment and diagnosis of these life-threatening infections.

Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates

2014 ◽  
Vol 59 (3) ◽  
Author(s):  
Monika Derda ◽  
Agnieszka Wojtkowiak-Giera ◽  
Edward Hadaś
Keyword(s):  
Genetic Markers ◽  
Tap Water ◽  
Correct Diagnosis ◽  
Specific Marker ◽  
Pcr Assay ◽  
Free Living ◽  
Detection And Identification ◽  
Granulomatous Amoebic Encephalitis ◽  
Free Living Amoeba ◽  
Treatment Of Infection

AbstractAcanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae strains. The aim of our study was to develop a rapid method for detection and identification of pathogenic Acanthamoeba spp. strains from diagnostic material collected from water. In this study we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP, Nelson) used for identification of pathogenic Acanthamoeba spp. strains isolated in water sources in Poland, Iceland and Sweden. Our results demonstrated the presence of pathogenic Acanthamoeba strains in tap water. PCR assay appeared to be a more rapid and sensitive method to detect the presence of amoebae than the limited conventional techniques. Based on our observations, we can confirm that the use of four out of five genetic markers (Aca 16S, Ac 6/210, JDP, GP, Nelson) may be helpful in identification of Acanthamoeba spp. strains, but only one Aca 16S primer pair is a highly specific marker that distinguishes between pathogenic strains of Acanthamoeba and other free-living amoeba families.

Development of a high- versus low-pathogenicity model of the free-living amoeba Naegleria fowleri

Microbiology ◽  
2012 ◽  
Vol 158 (10) ◽  
pp. 2652-2660 ◽  
Author(s):  
Denise C. Burri ◽  
Bruno Gottstein ◽  
Béatrice Zumkehr ◽  
Andrew Hemphill ◽  
Nadia Schürch ◽  
...  
Keyword(s):  
Naegleria Fowleri ◽  
Free Living ◽  
Free Living Amoeba ◽  
Nægleria Fowleri

An Electron Microscope study of a Small Free-living Amoeba (Hartmanella astronyxis)

1959 ◽  
Vol s3-100 (49) ◽  
pp. 13-15
Author(s):  
K. DEUTSCH ◽  
M. M. SWANN
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Cell Membrane ◽  
Nuclear Membrane ◽  
Layered Structure ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Honeycomb Structure ◽  
Free Living ◽  
Free Living Amoeba

The fine structure of a species of small free-living amoeba, Hartmanella astronyxis, has been investigated. The mitochondria resemble those of other species of amoeba. Structureless bodies of about the same size as mitochondria are sometimes found in association with them. Double membranes are common in the cytoplasm, and may show granules along their outer borders. The nuclear membrane is a double-layered structure, with a honeycomb structure evident in tangential sections. The cell membrane is also double-layered, or occasionally multi-layered.

Incidence of free living amoeba in different water sources in el-minia governorate (middle egypt)

1998 ◽  
Vol 47 ◽  
pp. 159
Author(s):  
Khalifa M.A. Refaat ◽  
Gabr S. Nabil ◽  
Abedel-lateef Z.M. Manal
Keyword(s):  
Water Sources ◽  
Free Living ◽  
Free Living Amoeba ◽  
Different Water Sources

scholarly journals Investigating the frequency of Free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020

2020 ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective: These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results: Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enabled us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study.

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