Effect of different calcium concentration on sperm motility and fertilisation capacity of rainbow trout ( Oncorhynchus mykiss )

2020 ◽  
Author(s):  
Leydy Sandoval‐Vargas ◽  
Elías Figueroa Villalobos ◽  
Pablo Contreras ◽  
Francisco Estay ◽  
Iván Valdebenito Isler
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Calcium Concentration ◽  
Rainbow Trout Oncorhynchus Mykiss

Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

Cryobiology ◽  
2014 ◽  
Vol 69 (3) ◽  
pp. 462-466 ◽  
Author(s):  
Filiz Kutluyer ◽  
Murathan Kayim ◽  
Fatih Öğretmen ◽  
Serhat Büyükleblebici ◽  
P. Barbaros Tuncer
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Rainbow Trout Oncorhynchus Mykiss

146 Effect of pH on Rainbow trout (Oncorhynchus mykiss) Sperm Motility Using Five Extender Solutions

2018 ◽  
Vol 30 (1) ◽  
pp. 213
Author(s):  
M. Á. Peralta-Martínez ◽  
S. R. García ◽  
M. E. Kjelland ◽  
H. González-Márquez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Salt Solution ◽  
Pond Water ◽  
Effect Of Ph ◽  
Sperm Activation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Abdominal Massage ◽  
Sexually Mature

Rainbow trout (Oncorhynchus mykiss) sperm extender protocols can differ considerably with regards to composition of the extenders and handling. The objective of this study was to determine the effect of pH for 5 extenders on rainbow trout sperm motility and activation during storage at 5°C. Two-year-old sexually mature rainbow trout males (n = 46) weighing 1.5 to 2.5 kg were caught at Aquaculture Farm Tatakay in Jilotzingo, Estado de México, México. One semen sample per male was collected by abdominal massage. For the experiments, 100 mL of each extender (306, 512, Mounibs, Erdahl and Graham, and Hanks’ Balanced Salt Solution) was made, with pH adjusted to 6 levels (7.0, 7.2, 7.4, 7.8, 8.0, and 8.2). To evaluate pH dynamics in each extender, pH was tested daily over an 8-day storage interval. Trout sperm was added to each of the extenders in a ratio of 1:1 and motility recorded. Afterwards, each activator solution [DIA 532, saline solution (0.85%) and pond water] was added separately to a sample of the extended sperm, to initiate sperm activation. Motility was evaluated subjectively at 400× and monitored until ~99% of the sperm stopped moving (on average, in 40 s). A one-way ANOVA was used and statistical differences were set at α < 0.05. Extender 512 activated sperm motility when pH was >7.4, whereas extender 306 activated motility at every pH. Hanks’ Balanced Salt Solution activated motility at very low percentages for various pH levels, whereas Mounibs and Erdahal and Graham solutions did not activate motility for any pH tested. The 512 extender with a pH of 7 performed best (P < 0.05) as a storage solution, producing a sperm motility of 54% after activation using DIA 532. These results demonstrated the importance of evaluating effects of sperm extender pH over both short- and medium-term storage.

Fertilization dynamics in rainbow trout (Oncorhynchus mykiss): effect of male age, social experience, and sperm concentration and motility on in vitro fertilization

2002 ◽  
Vol 59 (1) ◽  
pp. 144-152 ◽  
Author(s):  
N R Liley ◽  
P Tamkee ◽  
R Tsai ◽  
D J Hoysak
Keyword(s):  
Rainbow Trout ◽  
Reproductive Success ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Critical Role ◽  
Sperm Concentration ◽  
Adult Males ◽  
Vitro Fertilization ◽  
Rainbow Trout Oncorhynchus Mykiss

We examine aspects of the fertilization dynamics of rainbow trout (Oncorhynchus mykiss) that may play a role in determining reproductive success of males of different age and status competing for spawning. There were no differences in the gonadosomatic indices and relative yields of milt of adult (3-year) and precocious (1-year) male rainbow trout collected from a wild population. The concentration of sperm in the milt of precocious males was higher than that of adult males. The duration of sperm motility was similar in the two groups of males and increased over the period of sampling. Interaction of a male with a nesting female caused an increase in milt yield, but did not affect sperm concentration, sperm motility, or fertilization rates. There was a sharp decline in fertilization rate 20 s or more after activation of the sperm or eggs by fresh water. Exposure to milt suspension for as little as 0.5 s resulted in fertilization of <27% of eggs. The short gamete longevity and the speed with which fertilization occurs indicate that the timing and position of sperm release may play a critical role in determining the reproductive success of males in competition for spawning with a single female.

scholarly journals Improvement of Sperm Motility of Rainbow Trout (Oncorhynchus mykiss W., 1792) by Supplementation of L-Arginine

2019 ◽  
Vol 7 (9) ◽  
pp. 1300
Author(s):  
Durali Danabaş ◽  
Mehmet Kocabaş ◽  
Filiz Kutluyer
Keyword(s):  
Nitric Oxide ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Motile Sperm ◽  
Promoting Effect ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Amino Acid Precursor ◽  
Arginine Concentration ◽  
Acid Precursor

In this study, the effect of supplementing amino acid-precursor of nitric oxide (NO) L-arginine were examined on sperm motility of rainbow trout Oncorhynchus mykiss. Different concentrations [0 mM (Control), 1 mM, 2 mM, 4 mM and 8 mM] of L-arginine were used in the study. To assess the effects on percentage of motile sperm and longevity, L-arginine was added to activation medium containing NaCl (52 mM). The higher L-arginine concentration (4mM) has promoting-effect on sperm motility. The treatments containing L-arginine caused significant effect on percentage of motile sperm and longevity. Overall, the findings of the present study indicated that supplementation of L-arginine may have improving effects on sperm motility of rainbow trout.

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