scholarly journals Isolation and characterization of 21 novel microsatellite loci in sailfish Istiophorus platypterus Shaw 1792 from a shotgun genomic library

2018 ◽  
Vol 34 (4) ◽  
pp. 974-976
Author(s):  
G. G. Rubio-Castro ◽  
A. Munguia-Vega ◽  
C. Quiñonez-Velázquez ◽  
F. J. García-Rodríguez
Keyword(s):  
Microsatellite Loci ◽  
Genomic Library ◽  
Istiophorus Platypterus ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Microsatellite Locus ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Relationship Patterns ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

scholarly journals Isolation and Characterization of Nine Microsatellite Markers for Cymbidium sinense

HortScience ◽  
2008 ◽  
Vol 43 (6) ◽  
pp. 1925-1926 ◽  
Author(s):  
Kuaifei Xia ◽  
Xiulin Ye ◽  
Mingyong Zhang
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Molecular Breeding ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Germplasm Resources ◽  
Enrichment Method ◽  
Isolation And Characterization ◽  
Cymbidium Sinense

Microsatellite-enhanced genomic library of Cymbidium sinense was constructed using repeat-enrichment method with biotin-labeled oligos and streptavidin magnetic beads. Twenty-five microsatellite loci were isolated from a microsatellite-enhanced genomic library of Cymbidium sinense. Nine of these loci displayed genetic diversity that ranged from one to 15 alleles per locus, and the observed heterozygosity of six polymorphic loci ranged from 0.18 to 0.90 with an average of 0.47 (0.29 sd) in a sample of 30 individuals. Eight of these loci were successfully amplified in at least one of the following species: Paphiopedilum hissutissimum, P. wardii, P. armeniacum, or P. micranthum. The study provides a base for molecular breeding and assessment of germplasm resources of the Cymbidium sinense and the results suggest that the microsatellite loci developed from C. sinense may have a broad applicability within the Orchidaceae family.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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