Testing staining techniques to determine age and growth of Dasyatis pastinaca (Linnaeus, 1758) captured in Iskenderun Bay, northeastern Mediterranean

2016 ◽  
Vol 32 (3) ◽  
pp. 595-601 ◽  
Author(s):  
H. Girgin ◽  
N. Başusta
Keyword(s):  
Age And Growth ◽  
Iskenderun Bay ◽  
Staining Techniques

Age and growth of the blackchin guitarfish Glaucostegus cemiculus (Geoffroy Saint‐Hilaire, 1817) from Iskenderun Bay (Northeastern Mediterranean)

2020 ◽  
Vol 36 (6) ◽  
pp. 880-887
Author(s):  
Nuri Başusta ◽  
Asiye Başusta ◽  
Eyüp Mümtaz Tıraşın ◽  
James A. Sulikowski
Keyword(s):  
Age And Growth ◽  
Iskenderun Bay

Age and Growth of Red Sea Goatfish, Parupeneus forsskali from Iskenderun Bay, Northeastern Mediterranean Sea

2021 ◽  
Vol 61 (5) ◽  
pp. 758-763
Author(s):  
C. Turan ◽  
A. Ergenler ◽  
S. A. Doğdu ◽  
F. Turan
Keyword(s):  
Mediterranean Sea ◽  
Red Sea ◽  
Age And Growth ◽  
Iskenderun Bay

Age and growth of the common guitarfish, Rhinobatos rhinobatos, in Iskenderun Bay (north-eastern Mediterranean, Turkey)

2008 ◽  
Vol 88 (4) ◽  
pp. 837-842 ◽  
Author(s):  
Nuri Başusta ◽  
Sefa Ayhan Demirhan ◽  
Erdoğan Çiçek ◽  
Asiye Başusta ◽  
Tuncay Kuleli
Keyword(s):  
Eastern Mediterranean ◽  
Growth Curves ◽  
Mediterranean Coast ◽  
Age And Growth ◽  
Pooled Data ◽  
Total Length ◽  
The North ◽  
North Eastern ◽  
Iskenderun Bay ◽  
The Common

Age and growth of the common guitarfish (Rhinobatos rhinobatos) were estimated from the north-eastern Mediterranean coast of Turkey. A total of 115 common guitarfish (66 females and 49 males) were sampled. Age determinations were carried out using vertebral sections. Alcian blue dying techniques were used to enhance the visibility of the band on vertebrae. The techniques have been used for the first time on elasmobranchs vertebrae successfully in this study. A total of 97 vertebrae of 115 were visible on the vertebral band in this study. Male and female common guitarfish ranged in age from 1 to 15 and 1 to 24 y respectively. Total length ranged from 42 to 147 cm for females and 39 to 124 cm for males.The relationships between total length (TL) and total weight (TW) were presented as TW = 0.0014*TL3.1672 (R2 = 0.98) for females, TW = 0.0012*TL3.1947 (R2 = 0.98) for males and TW = 0.0012*TL3.1915 (R2 = 0.98) for both sexes. The parameters for von Bertalanffy growth curves were estimated as Linf  = 137.70±9.38 cm, K = 0.159±0.047, to = −2.180 ± 0.90 for pooled data.

Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae

2020 ◽  
Vol 84 (4) ◽  
pp. 343-354
Author(s):  
José G. Pérez-Rojas ◽  
Katherine Torres-Palacios ◽  
Amalia Uribe ◽  
Andrés F. Navia ◽  
Paola A. Mejía-Falla
Keyword(s):  
Age And Growth ◽  
Tropical Species ◽  
Shark Species ◽  
Growth Band ◽  
Sphyrna Lewini ◽  
Specific Protocol ◽  
Growth Bands ◽  
Staining Techniques ◽  
Hard Structures ◽  
Species Specific

The aim of this study was to assess the suitability of different vertebrae staining techniques for the visualization and counting of growth bands in tropical species of batoids (Narcine leoparda, Urotrygon aspidura, Hypanus longus, Potamotrygon magdalenae) and sharks (Alopias pelagicus, Carcharhinus falciformis, Sphyrna lewini, Sphyrna corona and Mustelus lunulatus). Different cutting thicknesses and staining protocols were tested, analysing the precision and bias of each combination to identify the most accurate technique for estimating age. Vertebral sections of 0.4 mm were more suitable for batoids, except for Narcine leoparda; for this species and for all the shark species assessed, sections of 0.5 mm are recommended. Different combinations of stain and exposure time were required to achieve the best visualizations of vertebral growth band pair for the shark and ray species. Intraspecific variation occurred among vertebrae size of batoids. Our results confirm the importance of defining a suitable species-specific protocol for sectioning and staining hard structures before carrying out an age and growth study to improve the reliability of the age estimates.

Observation of biological macromolecules using various electron microscopic methods

1978 ◽  
Vol 36 (2) ◽  
pp. 170-171
Author(s):  
Mitsuo Ohtsuki ◽  
Michael Sogard
Keyword(s):  
Electron Microscope ◽  
Phase Contrast ◽  
Image Interpretation ◽  
Density Lipoprotein ◽  
Biological Macromolecules ◽  
Contrast Effects ◽  
Biological Structure ◽  
Electron Microscopic ◽  
Structural Investigations ◽  
Staining Techniques

Structural investigations of biological macromolecules commonly employ CTEM with negative staining techniques. Difficulties in valid image interpretation arise, however, due to problems such as variability in thickness and degree of penetration of the staining agent, noise from the supporting film, and artifacts from defocus phase contrast effects. In order to determine the effects of these variables on biological structure, as seen by the electron microscope, negative stained macromolecules of high density lipoprotein-3 (HDL3) from human serum were analyzed with both CTEM and STEM, and results were then compared with CTEM micrographs of freeze-etched HDL3. In addition, we altered the structure of this molecule by digesting away its phospholipid component with phospholipase A2 and look for consistent changes in structure.

A Simple Dichromatic Stain for Plastic Embedded Tissues

1970 ◽  
Vol 28 ◽  
pp. 296-297 ◽  
Author(s):  
G. R. Mackay ◽  
M. L. Mead
Keyword(s):  
Electron Microscopy ◽  
Toluidine Blue ◽  
Biological Material ◽  
Good Reproducibility ◽  
Routine Work ◽  
Staining Techniques

Color contrasting of 1 to 2 micron sections of plastic embedded biological material is an important adjunct to electron microscopy. The procedures in general use today are simple and rapid giving monochromatic results, e.g., toluidine blue. Although many di- and polychromatic histologic staining techniques have been modified to obtain a counterstaining effect with plasticembedded tissue, the methods are usually undesirable for routine work because they are time consuming, complicated and often defy good reproducibility.

Effect of ADH on Rat Renal Papilla, an Ultrastructural and Cytochemical Study

1973 ◽  
Vol 31 ◽  
pp. 410-411
Author(s):  
C. N. Sun ◽  
H. J. White ◽  
E. J. Towbin
Keyword(s):  
Electron Microscopy ◽  
Diabetes Insipidus ◽  
Renal Papilla ◽  
Milk Diet ◽  
Intercellular Spaces ◽  
Cytochemical Study ◽  
Collecting Tubule ◽  
Concentrate Urine ◽  
Staining Techniques ◽  
Collecting Tubules

Diabetes insipidus and compulsive water drinking are representative of two categories of antidiuretic hormone (ADH) lack. We studied a strain of rats with congenital diabetes insipidus homozygote (DI) and normal rats on an isocaloric fortified dilute milk diet. In both cases, the collecting tubules could not concentrate urine. Special staining techniques, Alcian Blue-PAS for light microscopy and lanthanum nitrate for electron microscopy were used to demonstrate the changes in interstitial mucopolysaccharides (MPS). The lanthanum staining was done according to the method of Khan and Overton.Electron microscopy shows cytoplasmic lesions, vacules, swelling and degenerating mitochondria and intercellular spaces (IS) in the collecting tubule cells in DI and rats on milk diet.

Polymer Morphology Revealed by Staining Techniques

1981 ◽  
Vol 39 ◽  
pp. 340-341
Author(s):  
A. C. Reimschuessel ◽  
V. Kramer
Keyword(s):  
Polymer Melt ◽  
Nylon 6 ◽  
Morphological Features ◽  
Negative Staining ◽  
Polymer Morphology ◽  
Crystallizable Polymer ◽  
Staining Techniques ◽  
Long Chain

Staining techniques can be used for either the identification of different polymers or for the differentiation of specific morphological domains within a given polymer. To reveal morphological features in nylon 6, we choose a technique based upon diffusion of the staining agent into accessible regions of the polymer.When a crystallizable polymer - such as nylon 6 - is cooled from the melt, lamellae form by chainfolding of the crystallizing long chain macromolecules. The regions between adjacent lamellae represent the less ordered amorphous domains into which stain can diffuse. In this process the lamellae will be “outlined” by the dense stain, giving rise to contrast comparable to that obtained by “negative” staining techniques.If the cooling of the polymer melt proceeds relatively slowly - as in molding operations - the lamellae are usually arranged in a radial manner. This morphology is referred to as spherulitic.

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