Changes in Water Status, Membrane Stability and Antioxidant Capacity of Wheat Seedlings Carrying Different Rht-B1 Dwarfing Alleles under Drought Stress

2013 ◽  
Vol 200 (2) ◽  
pp. 83-91 ◽  
Author(s):  
K. Kocheva ◽  
V. Nenova ◽  
T. Karceva ◽  
P. Petrov ◽  
G. I. Georgiev ◽  
...  
Keyword(s):  
Drought Stress ◽  
Antioxidant Capacity ◽  
Water Status ◽  
Membrane Stability ◽  
Wheat Seedlings

Fructan dynamics and antioxidant capacity of 4-day-old seedlings of wheat (Triticum aestivum) cultivars during drought stress and recovery

2018 ◽  
Vol 45 (10) ◽  
pp. 1000 ◽  
Author(s):  
Farnoosh Nemati ◽  
Faezeh Ghanati ◽  
Hassan Ahmadi Gavlighi ◽  
Mohsen Sharifi
Keyword(s):  
Drought Stress ◽  
Antioxidant Capacity ◽  
Membrane Lipids ◽  
Radical Scavenging ◽  
Oh Radical ◽  
Drought Condition ◽  
Wheat Seedlings ◽  
Degree Of Polymerisation ◽  
Enhanced Production ◽  
Wide Range

One of the inevitable consequences of drought stress is enhanced production of reactive oxygen species (ROS). Fructan might function as effective candidate for capturing ROS in a wide range of stresses. Herein, 4-day-old seedlings of drought-tolerant and -sensitive wheat cultivars were exposed to drought stress for 7 days by water cessation, followed by further 7 days re-watering. The content, metabolism, related enzymes activity, degree of polymerisation (DP) and antioxidant capacity of fructan were compared in the two cultivars. High resolution HPAEC-PAD analysis of fructan showed an increase in the activities of fructan: fructan 1-fructosyltransferase (1-FFT) in the tolerant cultivar and sucrose: sucrose 1-fructosyltransferase (1-SST) and 1-FFT in the sensitive cultivar under drought condition. The activity of fructan exohydrolase (FEH) did not show any significant change in tolerant cultivar, but decreased in a sensitive one. In comparison with the sensitive cultivar, the tolerant one accumulated fructan (0.9% of dry matter) with higher degree of polymerisation (10.67 ± 1.1), accompanied by increased OH radical scavenging activity, during drought condition. In regard to the fact that OH radical is the most prevalent ROS in damaging membrane lipids, the results suggest that fructans play a crucial role in the tolerance of wheat seedlings against drought stress.

Effects of Betaine on Chloroplast Protective Enzymes andpsbAGene Expression in Wheat Seedlings under Drought Stress

2013 ◽  
Vol 39 (7) ◽  
pp. 1319 ◽  
Author(s):  
Peng-Fei HOU ◽  
Jun-Qing MA ◽  
Peng-Fei ZHAO ◽  
Huan-Ling ZHANG ◽  
Hui-Jie ZHAO ◽  
...  
Keyword(s):  
Drought Stress ◽  
Wheat Seedlings ◽  
Protective Enzymes

scholarly journals Enhancement of drought tolerance in Triticum aestivum L. seedlings using Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Wedad A. Kasim ◽  
Mohamed E. H. Osman ◽  
Mohamed N. Omar ◽  
Samar Salama
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Azospirillum Brasilense ◽  
Stenotrophomonas Maltophilia ◽  
Protein Pattern ◽  
Triticum Aestivum L ◽  
Protein Patterns ◽  
Wheat Seedlings ◽  
Enhanced Production

Abstract Background The effectiveness of two PGPB; Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11 was investigated in enhancing the drought tolerance of wheat (Triticum aestivum L.) seedlings cultivar Gemiza9. The inoculated or uninoculated grains were sown in unsterilized sandy soil and watered normally untill the 8th day. Drought stress was initiated by completely withholding water for 7 days (until wilting). Samples were collected after 15 days from sowing to evaluate some growth criteria, damage and defense indicators and to analyze the roots’ protein pattern. Results The results showed that inoculating wheat seedlings with these strains significantly diminished the inhibitory effects of drought stress on the relative water content of roots, shoots and leaves; area of leaves; contents of pigments (chlorophyll a and b) and ascorbic acid; and on the protein patterns of roots. Moreover, the bacterial inoculation notably reduced the drought-induced damage indicated by lower leakage of electrolytes and less accumulation of Malondialdehyde and hydrogen peroxide, surprisingly with less enhanced production of proline and activities of catalase and peroxidase than their uninoculated counterparts. Under normal conditions, inoculating wheat plants with these PGPB resulted in significantly promoted growth and elevated contents of pigments and altered protein patterns of roots. Conclusion Overall, we can say that both Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11 were able to deactivate the growth inhibition in wheat seedlings to some extent, while maintaining a certain level of efficient protection against damage under drought stress.

scholarly journals DEHYDRIN PROFILES OF SOME IRANIAN MELON VARIETIES (Cucumis melo L. Merr) UNDER DROUGHT STRE SS CONDITIONS

2019 ◽  
Vol 18 (6) ◽  
pp. 75-84
Author(s):  
Alireza Motallebi-Azar ◽  
István Papp ◽  
Anita Szegő
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Water Content ◽  
Water Status ◽  
Field Capacity ◽  
Total Protein Content ◽  
Severe Drought ◽  
Molecular Weights ◽  
Potential Marker ◽  
Relative Water

Dehydrins are proteins that play a role in the mechanism of drought tolerance. This study aimed at establishing dehydrin profile and accumulation in four local melon varieties of Iran: Mino, Dargazi, Saveii, and Semsori, as well as in a commercial variety Honeydew. Plants were treated with drought stress by adjusting the soil water content to 75, 50, 40, 30 and 20% of field capacity (FC) by withholding water. Water status of plants was monitored based on the seedling fresh weight (FW) and relative water content of leaves (RWC). Total protein content was extracted, then heat-stable protein (HSP) fraction was isolated for each variety and water stress treatment. After SDS-PAGE of HSP, Western blotting analysis was carried out with Anti-dehydrin rabbit (primary) and Goat anti rabbit (secondary) antibodies. ANOVA results showed that with decreasing FC below 75%, FW and RWC decreased, but these changes significantly varied among genotypes. On the basis of FW and RWC data under different drought stress treatments, the following drought-tolerant ranking was established: Mino > Dargazi > Saveii and Honeydew > Semsori, from tolerant to sensitive order. Results of Western blot analysis showed that expression of some proteins with molecular weights of 19–52 kDa was induced in the studied varieties under drought stress (% FC). Expression level of the dehydrin proteins in different varieties was variable and also depending on the drought stress level applied. However, dehydrin proteins (45 and 50 kDa) showed strong expression levels in all varieties under severe drought stress (20% FC). The abundance of dehydrin proteins was higher in tolerant varieties (Mino and Dargazi) than in moderate and drought sensitive genotypes. Consequently, dehydrins represent a potential marker for selection of genotypes with enhanced drought tolerance.

Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress

2018 ◽  
Vol 164 ◽  
pp. 611-617 ◽  
Author(s):  
Zongbo Qiu ◽  
Yanyan He ◽  
Yimeng Zhang ◽  
Junli Guo ◽  
Li Wang
Keyword(s):  
Drought Stress ◽  
Target Genes ◽  
Wheat Seedlings

scholarly journals Physiological Responses under Drought Stress of Improved Drought-Tolerant Rice Lines and their Parents

2018 ◽  
Vol 46 (2) ◽  
pp. 679-687 ◽  
Author(s):  
Preeyanuch LARKUNTHOD ◽  
Noppawan NOUNJAN ◽  
Jonaliza L SIANGLIW ◽  
Theerayut TOOJINDA ◽  
Jirawat SANITCHON ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Water Status ◽  
Physiological Responses ◽  
High Water ◽  
Recurrent Parent ◽  
Potential Candidate ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Drought Tolerant

Many of the economically important rice cultivars including ‘Khao Dawk Mali 105’ (KDML105) or jasmine rice, one of the world’s famous rice exported from Thailand suffers from drought due to erratic rainfalls and limited irrigation. To improve drought tolerance and reserve genetic background of KDML105, chromosome segment substitution lines (CSSL) containing drought tolerant quantitative trait loci (DT-QTL) has been previously developed by backcrossing between KDML105 and drought tolerant donor, IR58586-F2-CA-143 (DH212). To understand the physiological responses related to drought tolerance in CSSL lines compared to parents, two CSSLs namely CSSL1-16 and CSSL1-18, respectively were used in this study. Twenty-one-d-old hydroponically grown plants were subjected to 20% PEG for 0, 7, 14 d and then recovered from stress for 3 d. The results indicated that CSSL lines especially, CSSL1-16 showed better performance under drought stress compared to their recurrent parent. Drought tolerance superior CSSL1-16 line was indicated by high water status (high relative water content and leaf water potential), good osmotic adjustment, high proline and greater membrane stability. Moreover, this line was able to resume growth after stress recovery whereas other lines/cultivar could not recover. Similarly, drought tolerant donor showed high water status suggesting that well-maintained plant water status was associated with drought tolerant trait. It could be concluded that the highest drought tolerant line was CSSL1-16 followed by DH212, CSSL1-18 and KDML105. It would be interesting to go further into introgressed section in CSSL1-16 to identify potential candidate genes in DT-QTL for breeding drought tolerant rice in the future.

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