scholarly journals Cryopreservation of lipoaspirates: in vitro measurement of the viability of adipose‐derived stem cell and lipid peroxidation

2020 ◽  
Vol 17 (5) ◽  
pp. 1282-1290
Author(s):  
Dong Yeon Kim ◽  
Eunjin Kim ◽  
Ki Joo Kim ◽  
Young‐Joon Jun ◽  
Jong‐Won Rhie
Keyword(s):  
Lipid Peroxidation ◽  
Stem Cell ◽  
Adipose Derived Stem Cell

3D Human Adipose-Derived Stem Cell Clusters as a Model for In Vitro Fibrosis

2016 ◽  
Vol 22 (7) ◽  
pp. 679-690 ◽  
Author(s):  
Thanavel Rajangam ◽  
Min Hee Park ◽  
Sang-Heon Kim
Keyword(s):  
Stem Cell ◽  
Cell Clusters ◽  
Adipose Derived Stem Cell

An in vitro cellular system modelling progressive human adipose-derived stem cell aging

2018 ◽  
Vol 63 (5) ◽  
pp. 272-274 ◽  
Author(s):  
Yanghua Shi ◽  
Lian Wang ◽  
Yichang Li ◽  
Congdi Xu ◽  
Xiaowen Shao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cellular System ◽  
Cell Aging ◽  
System Modelling ◽  
Stem Cell Aging ◽  
Adipose Derived Stem Cell

scholarly journals Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Xiaoru Xu ◽  
Kaixiu Fang ◽  
Lifeng Wang ◽  
Xiangwei Liu ◽  
Yuchao Zhou ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Stem Cell ◽  
Bone Healing ◽  
Cell Sheet ◽  
Semaphorin 3A ◽  
Adipose Derived Stem Cell

Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) and Bio-Oss® bone granules on bone healing in type 2 diabetes mellitus (T2DM) rats with the addition of semaphorin 3A (Sema3A). The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. An ASC sheet combined with Bio-Oss® bone granules promoted bone formation in T2DM rats as indicated by microcomputed tomography (micro-CT) and histological analysis. In addition, Sema3A promoted the osteogenic differentiation of ASC sheets in vitro and local injection of Sema3A promoted T2DM rats’ calvarial bone regeneration based on ASC sheet and Bio-Oss® bone granule complex treatment. In conclusion, the local injection of Sema3A and the complexes of ASC sheet and Bio-Oss® bone granules could promote osseous healing and are potentially useful to improve bone healing for T2DM patients.

The effect of exogenous histone H1 on rat adipose-derived stem cell proliferation, migration, and osteogenic differentiation in vitro

2012 ◽  
Vol 227 (10) ◽  
pp. 3417-3425 ◽  
Author(s):  
Li-Wen Hsu ◽  
Shigeru Goto ◽  
Toshiaki Nakano ◽  
Kuang-Den Chen ◽  
Chih-Chi Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Histone H1 ◽  
Stem Cell Proliferation ◽  
Exogenous Histone ◽  
Adipose Derived Stem Cell

In-vitro cell adhesion and proliferation of adipose derived stem cell on hydroxyapatite composite surfaces

2017 ◽  
Vol 75 ◽  
pp. 1305-1316 ◽  
Author(s):  
Praneetha Pulyala ◽  
Akshay Singh ◽  
Marcela Ferreira Dias-Netipanyj ◽  
Sheron Compos Cogo ◽  
Luciane S. Santos ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Hydroxyapatite Composite ◽  
Adipose Derived Stem Cell ◽  
Cell Adhesion And Proliferation

Effects of 3D-bioplotted polycaprolactone scaffold geometry on human adipose-derived stem cell viability and proliferation

2017 ◽  
Vol 23 (3) ◽  
pp. 534-542 ◽  
Author(s):  
Saahil V. Mehendale ◽  
Liliana F. Mellor ◽  
Michael A. Taylor ◽  
Elizabeth G. Loboa ◽  
Rohan A. Shirwaiker
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Pore Size ◽  
Compression Modulus ◽  
Engineering Applications ◽  
Content Type ◽  
Musculoskeletal Tissue ◽  
Adipose Derived Stem Cell

Purpose This study aims to investigate the effect of three-dimensional (3D)- bioplotted polycaprolactone (PCL) scaffold geometry on the biological and mechanical characteristics of human adipose-derived stem cell (hASC) seeded constructs. Design/methodology/approach Four 3D-bioplotted scaffold disc designs (Ø14.5 × 2 mm) with two levels of strand–pore feature sizes and two strand laydown patterns (0°/90° or 0°/120°/240°) were evaluated for hASC viability, proliferation and construct compressive stiffness after 14 days of in vitro cell culture. Findings Scaffolds with the highest porosity (smaller strand–pore size in 0°/120°/240°) yielded the highest hASC proliferation and viability. Further testing of this design in a 6-mm thick configuration showed that cells were able to penetrate and proliferate throughout the scaffold thickness. The design with the lowest porosity (larger strand–pore size in 0°/90°) had the highest compression modulus after 14 days of culture, but resulted in the lowest hASC viability. The strand laydown pattern by itself did not influence the compression modulus of scaffolds. The 14-day cell culture also did not cause significant changes in compressive properties in any of the four designs. Originality/value hASC hold great potential for musculoskeletal tissue engineering applications because of their relative ease of harvest, abundance and differentiation abilities. This study reports on the effects of 3D-bioplotted scaffold geometry on mechanical and biological characteristics of hASC-seeded PCL constructs. The results provide the basis for future studies which will use this optimal scaffold design to develop constructs for hASC-based osteochondral tissue engineering applications.

scholarly journals Adipose-derived stem cell-secreted factors promote early stage follicle development in a biomimetic matrix

2019 ◽  
Vol 7 (2) ◽  
pp. 571-580 ◽  
Author(s):  
Claire E. Tomaszewski ◽  
Elizabeth Constance ◽  
Melissa M. Lemke ◽  
Hong Zhou ◽  
Vasantha Padmanabhan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Early Stage ◽  
Follicle Development ◽  
Peg Hydrogels ◽  
Secreted Factors ◽  
Survival And Growth ◽  
Growth Promoting ◽  
Adipose Derived Stem Cell

ADSCs encapsulated in biomimetic PEG hydrogels maintain stemness and secrete survival- and growth-promoting factors that support in vitro folliculogenesis.

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