scholarly journals Wound‐healing effect of adipose stem cell‐derived extracellular matrix sheet on full‐thickness skin defect rat model: Histological and immunohistochemical study

2018 ◽  
Vol 16 (1) ◽  
pp. 286-296 ◽  
Author(s):  
Yoon J. Lee ◽  
Seung E. Baek ◽  
Sujin Lee ◽  
Yeon J. Jeong ◽  
Ki J. Kim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Stem Cell ◽  
Rat Model ◽  
Immunohistochemical Study ◽  
Skin Defect ◽  
Full Thickness ◽  
Adipose Stem Cell ◽  
Thickness Skin ◽  
Wound Healing Effect

Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat

2006 ◽  
Vol 17 (6) ◽  
pp. 547-552 ◽  
Author(s):  
Dae-Hyun Roh ◽  
Seuk-Yun Kang ◽  
Jeom-Yong Kim ◽  
Young-Bae Kwon ◽  
Hae Young Kweon ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Skin Defect ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Healing Effect ◽  
Thickness Skin ◽  
Wound Healing Effect

Effects of Nanofiber/Stem Cell Composite on Wound Healing in Acute Full-Thickness Skin Wounds

2011 ◽  
Vol 17 (9-10) ◽  
pp. 1413-1424 ◽  
Author(s):  
Kun Ma ◽  
Susan Liao ◽  
Liumin He ◽  
Jia Lu ◽  
Seeram Ramakrishna ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

Application of acellular intima from porcine thoracic aorta in full-thickness skin wound healing in a rat model

2017 ◽  
Vol 71 ◽  
pp. 1135-1144 ◽  
Author(s):  
Haitian Fu ◽  
Liping Teng ◽  
RuiZhen Bai ◽  
Chao Deng ◽  
Guozhong Lv ◽  
...  
Keyword(s):  
Wound Healing ◽  
Thoracic Aorta ◽  
Rat Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Conservative Management of Full-Thickness Skin Defect on Foot Dorsal Region: A Report of 2 Cases Receiving Modified Moist Wound Healing Method

2010 ◽  
Vol 59 (3) ◽  
pp. 541-544
Author(s):  
Katsuro Fukuyama ◽  
Masaaki Imabayashi ◽  
Hirohumi Ohsako ◽  
Yuichiro Yazaki ◽  
Takashi Murayama ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conservative Management ◽  
Skin Defect ◽  
Full Thickness ◽  
Dorsal Region ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Experimental evaluation of the effectiveness of adipose mesenchymal stem cells in full-thickness skin wounds

2021 ◽  
Vol 9 (2) ◽  
pp. 175-181
Author(s):  
Ilya V. Artsimovich ◽  
Evgenii V. Zinoviev ◽  
Aleksander V. Gostimskii ◽  
Marat S. Asadulaev ◽  
Sergey V. Vissarionov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Control Group ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Healing Index ◽  
Thickness Skin

BACKGROUND: Currently, numerous techniques or medical devices that allow complete or partial restoration of the lost skin within a short time remain as subjects of development. Many studies have shown the effectiveness of using stem cells in the treatment of full-thickness skin defects, but their use remains very limited. At present, there is no consensus among researchers about the advisability of the use of stem cells in the treatment of burns as well as about the method of their introduction. AIM: This study aimed to examine the possibility of accelerating the reparative histogenesis of tissues in the zone of deep skin burns using cultures of adipogenic mesenchymal cells, as well as to evaluate the effectiveness of various methods of introducing cultures of these cells into the defect zone. MATERIALS AND METHODS: An experimental study was carried out on small laboratory animals (rats). After simulating a grade III burn, mesenchymal stem cells were transplanted and superficially applied to the wound surface or injected under the fascia. In the control group, no wound treatment was performed. To assess the effectiveness of the wound-healing preparations, the appearance of wounds was assessed daily, the nature of the discharge and presence and type of granulations were noted, and the timing of scab rejection and wound healing was recorded. The wound area was assessed using a planimetric method. A histological examination of wound biopsies was carried out on days 7, 14, 21, and 28 of observation. RESULTS: The application of adipogenic mesenchymal stem cells demonstrated the greatest efficiency on the developed burn model. Subfascial administration was less effective, but this method achieved a significant acceleration of wound healing in comparison with the control group. An increase in the healing index by 56.6% demonstrates the highest intensity of reparative regeneration in animals applied with adipogenic mesenchymal stem cells. CONCLUSIONS: The preliminary results show that the application of adipogenic mesenchymal stem cells on the skin defect is more effective than subfascial administration based on the healing index. The use of adipogenic mesenchymal stem cells may substantially increase the effectiveness of the treatment of full-thickness skin defects.

scholarly journals One-step approach for full-thickness skin defect reconstruction in rats using minced split-thickness skin grafts with Pelnac overlay

2019 ◽  
Vol 7 ◽  
Author(s):  
Tong Liu ◽  
Chao Qiu ◽  
Chi Ben ◽  
Haihang Li ◽  
Shihui Zhu
Keyword(s):  
Wound Healing ◽  
Healing Rate ◽  
Skin Grafting ◽  
Skin Defect ◽  
Skin Grafts ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Split Thickness Skin Grafting

Abstract Background Split-thickness skin grafting is the current gold standard for the treatment of traumatic skin loss. However, for patients with extensive burns, split-thickness skin grafting is limited by donor skin availability. Grafting split-thickness skin minced into micrografts increases the expansion ratio but may reduce wound repair quality. Dermal substitutes such as Pelnac can enhance the healing of full-thickness skin wounds, but their application currently requires two surgeries. The present study investigated whether it is possible to repair full-thickness skin defects and improve wound healing quality in a single surgery using Pelnac as an overlay of minced split-thickness skin grafts in a rat model. Methods A full-thickness skin defect model was established using male Sprague-Dawley rats of 10 weeks old. The animals were randomly divided into control and experimental groups in which Vaseline gauze and Pelnac, respectively, were overlaid on minced split-thickness skin grafts to repair the defects. Wound healing rate and quality were compared between the two groups. For better illustration of the quality of wound healing, some results were compared with those obtained for normal skin of rats. Results We found that using Pelnac as an overlay for minced split-thickness skin grafts accelerated wound closure and stimulated cell proliferation and tissue angiogenesis. In addition, this approach enhanced collagen synthesis and increased the formation of basement membrane and dermis as well as the expression of growth factors related to wound healing while reducing scar formation. Conclusions Using minced split-thickness skin grafts overlaid with Pelnac enables the reconstruction of full-thickness skin defects in a single step and can increase the healing rate while improving the quality of wound healing.

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