scholarly journals Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor

2017 ◽  
Vol 15 (1) ◽  
pp. 123-132 ◽  
Author(s):  
Hannah Trøstrup ◽  
Christian J. Lerche ◽  
Lars J. Christophersen ◽  
Kim Thomsen ◽  
Peter Ø. Jensen ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wound Healing ◽  
Growth Factor ◽  
Epithelial Growth Factor ◽  
Vascular Epithelial Growth Factor ◽  
Epithelial Growth

scholarly journals Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1303
Author(s):  
Edgar D. Moyers-Montoya ◽  
René Gerardo Escobedo-González ◽  
Claudia L. Vargas-Requena ◽  
Perla Elvia Garcia-Casillas ◽  
Carlos A. Martínez-Pérez
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Growth Factor ◽  
Murine Model ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Epithelial Growth Factor ◽  
Epithelial Growth

Polycaprolactone (PCL) is a well-known FDA approved biomaterial for tissue engineering. However, its hydrophobic properties limit its use for skin wound healing which makes its functionalization necessary. In this work, we present the fabrication and evaluation of PCL nanofibers by the electrospinning technique, as well as PCL functionalized with 6-deoxy-6-amino-β-cyclodextrin (aminated nanofibers). Afterwards, epithelial growth factor (EGF) was anchored onto hydrophilic PCL/deoxy-6-amino-β-cyclodextrin. The characterization of the three electrospun fibers was made by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR); Confocal-Raman Spectroscopy were used for elucidated the chemical structure, the hydrophilicity was determined by Contact Angle (CA). In vitro cell proliferation test was made by seeding embryonic fibroblast cell line (3T3) onto the electrospun mats and in vivo studies in a murine model were conducted to prove its effectivity as skin wound healing material. The in vitro studies showed that aminated nanofibers without and with EGF had 100 and 150% more cell proliferation of 3T3 cells against the PCL alone, respectively. In vivo results showed that skin wound healing in a murine model was accelerated by the incorporation of the EGF. In addition, the EGF had favorable effects in epidermal cell proliferation. The study demonstrates that a protein of high biological interest like EGF can be attached covalently to the surface of a synthetic material enriched with amino groups. This kind of biomaterial has a great potential for applications in skin regeneration and wound healing.

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