scholarly journals The regenerative role of adipose-derived stem cells (ADSC) in plastic and reconstructive surgery

2016 ◽  
Vol 14 (1) ◽  
pp. 112-124 ◽  
Author(s):  
Naghmeh Naderi ◽  
Emman J Combellack ◽  
Michelle Griffin ◽  
Tina Sedaghati ◽  
Muhammad Javed ◽  
...  
Keyword(s):  
Stem Cells ◽  
Reconstructive Surgery ◽  
Adipose Derived Stem Cells ◽  
Plastic And Reconstructive Surgery

scholarly journals Role of adipose-derived stem cells in fat grafting and reconstructive surgery

2016 ◽  
Vol 9 (3) ◽  
pp. 152 ◽  
Author(s):  
ShaunS Tan ◽  
Warren Rozen ◽  
ZhiYang Ng ◽  
Weiqing Zhan
Keyword(s):  
Stem Cells ◽  
Reconstructive Surgery ◽  
Fat Grafting ◽  
Adipose Derived Stem Cells

scholarly journals Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs

2020 ◽  
Vol 21 (17) ◽  
pp. 6181
Author(s):  
Sara Cruciani ◽  
Giuseppe Garroni ◽  
Francesca Balzano ◽  
Renzo Pala ◽  
Emanuela Bellu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vitamin D ◽  
Stem Cell ◽  
Cell Fate ◽  
Adipogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Vitamin D Metabolism ◽  
Stem Cell Fate ◽  
Cellular Phenotypes

Fat tissue represents an important source of adipose-derived stem cells (ADSCs), which can differentiate towards several phenotypes under certain stimuli. Definite molecules as vitamin D are able to influence stem cell fate, acting on the expression of specific genes. In addition, miRNAs are important modulating factors in obesity and numerous diseases. We previously identified specific conditioned media able to commit stem cells towards defined cellular phenotypes. In the present paper, we aimed at evaluating the role of metformin on ADSCs differentiation. In particular, ADSCs were cultured in a specific adipogenic conditioned medium (MD), in the presence of metformin, alone or in combination with vitamin D. Our results showed that the combination of the two compounds is able to counteract the appearance of an adipogenic phenotype, indicating a feedforward regulation on vitamin D metabolism by metformin, acting on CYP27B1 and CYP3A4. We then evaluated the role of specific epigenetic modulating genes and miRNAs in controlling stem cell adipogenesis. The combination of the two molecules was able to influence stem cell fate, by modulating the adipogenic phenotype, suggesting their possible application in clinical practice in counteracting uncontrolled lipogenesis and obesity-related diseases.

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