A non-destructive tissue sampling technique for holothurians to facilitate extraction of DNA for genetic analysis

2015 ◽  
Vol 134 (3) ◽  
pp. 252-259 ◽  
Author(s):  
Samantha J. Nowland ◽  
Dean R. Jerry ◽  
Paul C. Southgate
Keyword(s):  
Genetic Analysis ◽  
Sampling Technique ◽  
Tissue Sampling ◽  
Non Destructive

Intraductal aspiration: a promising new tissue-sampling technique for the diagnosis of suspected malignant biliary strictures

2012 ◽  
Vol 75 (4) ◽  
pp. 798-804 ◽  
Author(s):  
Gabriele Curcio ◽  
Mario Traina ◽  
Filippo Mocciaro ◽  
Rosa Liotta ◽  
Raffaella Gentile ◽  
...  
Keyword(s):  
Sampling Technique ◽  
Tissue Sampling ◽  
Biliary Strictures

scholarly journals Ultrasound-guided targeted biopsies of CT-based radiomic tumour habitats: technical development and initial experience in metastatic ovarian cancer

2020 ◽  
Author(s):  
Lucian Beer ◽  
Paula Martin-Gonzalez ◽  
Maria Delgado-Ortet ◽  
Marika Reinius ◽  
Leonardo Rundo ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ct Scan ◽  
Sampling Technique ◽  
Gaussian Mixture ◽  
Dice Similarity Coefficient ◽  
Tissue Sampling ◽  
Clinical Workflow ◽  
Registration Method ◽  
Contrast Enhanced Ct ◽  
Targeted Biopsies

Abstract Purpose To develop a precision tissue sampling technique that uses computed tomography (CT)–based radiomic tumour habitats for ultrasound (US)-guided targeted biopsies that can be integrated in the clinical workflow of patients with high-grade serous ovarian cancer (HGSOC). Methods Six patients with suspected HGSOC scheduled for US-guided biopsy before starting neoadjuvant chemotherapy were included in this prospective study from September 2019 to February 2020. The tumour segmentation was performed manually on the pre-biopsy contrast-enhanced CT scan. Spatial radiomic maps were used to identify tumour areas with similar or distinct radiomic patterns, and tumour habitats were identified using the Gaussian mixture modelling. CT images with superimposed habitat maps were co-registered with US images by means of a landmark-based rigid registration method for US-guided targeted biopsies. The dice similarity coefficient (DSC) was used to assess the tumour-specific CT/US fusion accuracy. Results We successfully co-registered CT-based radiomic tumour habitats with US images in all patients. The median time between CT scan and biopsy was 21 days (range 7–30 days). The median DSC for tumour-specific CT/US fusion accuracy was 0.53 (range 0.79 to 0.37). The CT/US fusion accuracy was high for the larger pelvic tumours (DSC: 0.76–0.79) while it was lower for the smaller omental metastases (DSC: 0.37–0.53). Conclusion We developed a precision tissue sampling technique that uses radiomic habitats to guide in vivo biopsies using CT/US fusion and that can be seamlessly integrated in the clinical routine for patients with HGSOC. Key Points • We developed a prevision tissue sampling technique that co-registers CT-based radiomics–based tumour habitats with US images. • The CT/US fusion accuracy was high for the larger pelvic tumours (DSC: 0.76–0.79) while it was lower for the smaller omental metastases (DSC: 0.37–0.53).

scholarly journals Disseminated cryptococcosis in a deceased with HIV‐1 diagnosed by minimally invasive tissue sampling technique

2021 ◽  
Author(s):  
Nuwadatta Subedi ◽  
Suraj Bhattarai ◽  
Sunita Ranabhat ◽  
Binita K. Sharma ◽  
Madan P. Baral ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Sampling Technique ◽  
Tissue Sampling ◽  
Hiv 1

Partial fin-clipping as an effective tool for tissue sampling seahorses, Hippocampus spp.

2011 ◽  
Vol 92 (6) ◽  
pp. 1427-1432 ◽  
Author(s):  
Lucy C. Woodall ◽  
Rachel Jones ◽  
Brian Zimmerman ◽  
Samantha Guillaume ◽  
Teague Stubbington ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Sampling Technique ◽  
Iucn Red List ◽  
Growth Time ◽  
Tissue Sampling ◽  
Short Term ◽  
Genetic Studies ◽  
Dorsal Fin ◽  
Significant Difference ◽  
Hippocampus Kuda

Partial fin-clipping is a non-lethal sampling technique commonly used to sample tissue for molecular genetic studies of fish. The effect of this technique was tested on seahorses (Hippocampus spp.) as they have several peculiar biological characteristics when compared with other fish and are on the IUCN Red List of Threatened Species. Partial fin-clipping of the seahorse dorsal fin was evaluated on Hippocampus kuda. The fish were assessed for short-term effects (fin re-growth time) as well as the longer term effects (growth and mortality) of partial fin clipping over a four month period. Total fin re-growth occurred between 2 and 4 weeks with no significant difference observed in the fin re-growth time between sexes. There was no significant difference between the mortality rate/growth rate of clipped versus unclipped seahorses. Results indicate partial fin-clipping has no significant effect on seahorses, and should be considered as a useful method for tissue sampling.

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