scholarly journals Cepheid Xpert ® Flu/RSV and Seegene Allplex ™ RP1 show high diagnostic agreement for the detection of influenza A/B and respiratory syncytial viruses in clinical practice

2020 ◽  
Author(s):  
Nasir Wabe ◽  
Robert Lindeman ◽  
Jeffrey J. Post ◽  
William Rawlinson ◽  
Melissa Miao ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Influenza A ◽  
Diagnostic Agreement ◽  
Respiratory Syncytial Viruses

scholarly journals Investigation of an outbreak of acute respiratory disease in an indigenous village in Brazil: Contribution of Influenza A(H1N1)pdm09 and human respiratory syncytial viruses

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0218925 ◽  
Author(s):  
Andrey Moreira Cardoso ◽  
Paola Cristina Resende ◽  
Enny S. Paixao ◽  
Felipe G. Tavares ◽  
Yasmin N. Farias ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Influenza A ◽  
Acute Respiratory Disease ◽  
Influenza A H1n1 ◽  
Respiratory Syncytial Viruses

scholarly journals Detection and Differentiation of SARS-CoV-2, Influenza, and Respiratory Syncytial Viruses by CRISPR

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 823
Author(s):  
Huifen Zhou ◽  
Jen-Hui Tsou ◽  
Molangur Chinthalapally ◽  
Hongjie Liu ◽  
Feng Jiang
Keyword(s):  
Early Detection ◽  
Influenza A ◽  
Respiratory Infections ◽  
Limit Of Detection ◽  
Respiratory Viruses ◽  
Cross Reaction ◽  
Sensitive Detection ◽  
Acute Respiratory Infections ◽  
Prevention And Treatment ◽  
Respiratory Syncytial Viruses

SARS-CoV-2, influenza, and respiratory syncytial viruses (RSVs) cause acute respiratory infections with similar symptoms. Since the treatments and outcomes of these infections are different, the early detection and accurate differentiation of the viruses are clinically important for the prevention and treatment of the diseases. We previously demonstrated that clustered regularly interspaced short palindromic repeats (CRISPR) could rapidly and precisely detect SARS-CoV-2. The objective of this study was to develop CRISPR as a test for simultaneously detecting and accurately distinguishing the viruses. The CRISPR assay with an RNA guide against each virus was performed in the reference standards of SARS-CoV-2, influenza A and B, and RSV. The CRISPR assay had a limit of detection of 1–100 copies/µL for specifically detecting SARS-CoV-2, influenza A and B, and RSV without cross-reaction with other respiratory viruses. The validation of the test in nasopharyngeal specimens showed that it had a 90–100% sensitivity and 100% specificity for the detection of SARS-CoV-2, influenza A and B, and RSV. The CRISPR assay could potentially be used for sensitive detection and specific differentiation of the respiratory viruses.

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