AIRE in context: Leveraging chromatin plasticity to trigger ectopic gene expression*

2021 ◽  
Author(s):  
Caroline Kaiser ◽  
Alexandra Bradu ◽  
Noah Gamble ◽  
Jason A. Caldwell ◽  
Andrew S. Koh
Keyword(s):  
Gene Expression ◽  
Ectopic Gene Expression

scholarly journals Easy and robust electrotransfection protocol for efficient ectopic gene expression and genome editing in human B cells

Gene Therapy ◽  
2020 ◽  
Author(s):  
Reynand Jay Canoy ◽  
Franck André ◽  
Anna Shmakova ◽  
Joëlle Wiels ◽  
Marc Lipinski ◽  
...  
Keyword(s):  
Gene Expression ◽  
B Cells ◽  
Genome Editing ◽  
Human B Cells ◽  
Ectopic Gene Expression

scholarly journals RB2/p130 ectopic gene expression in neuroblastoma stem cells: evidence of cell-fate restriction and induction of differentiation

2001 ◽  
Vol 360 (3) ◽  
pp. 569 ◽  
Author(s):  
Francesco P. JORI ◽  
Umberto GALDERISI ◽  
Elena PIEGARI ◽  
Gianfranco PELUSO ◽  
Marilena CIPOLLARO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Fate ◽  
Ectopic Gene Expression ◽  
Induction Of Differentiation ◽  
Fate Restriction

scholarly journals A modular chromosomally integrated toolkit for ectopic gene expression in Vibrio cholerae

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Triana N. Dalia ◽  
Jennifer L. Chlebek ◽  
Ankur B. Dalia
Keyword(s):  
Gene Expression ◽  
Vibrio Cholerae ◽  
Industrial Applications ◽  
Model System ◽  
Promoter Element ◽  
Reporter System ◽  
Dynamic Activity ◽  
Type Iv ◽  
Ectopic Gene Expression ◽  
Integrated Platform

Abstract The ability to express genes ectopically in bacteria is essential for diverse academic and industrial applications. Two major considerations when utilizing regulated promoter systems for ectopic gene expression are (1) the ability to titrate gene expression by addition of an exogenous inducer and (2) the leakiness of the promoter element in the absence of the inducer. Here, we describe a modular chromosomally integrated platform for ectopic gene expression in Vibrio cholerae. We compare the broadly used promoter elements Ptac and PBAD to versions that have an additional theophylline-responsive riboswitch (Ptac-riboswitch and PBAD-riboswitch). These constructs all exhibited unimodal titratable induction of gene expression, however, max induction varied with Ptac > PBAD > PBAD-riboswitch > Ptac-riboswitch. We also developed a sensitive reporter system to quantify promoter leakiness and show that leakiness for Ptac > Ptac-riboswitch > PBAD; while the newly developed PBAD-riboswitch exhibited no detectable leakiness. We demonstrate the utility of the tightly inducible PBAD-riboswitch construct using the dynamic activity of type IV competence pili in V. cholerae as a model system. The modular chromosomally integrated toolkit for ectopic gene expression described here should be valuable for the genetic study of V. cholerae and could be adapted for use in other species.

scholarly journals Response: Are Tissues a Patch Quilt of Ectopic Gene Expression?

Science ◽  
1989 ◽  
Vol 246 (4927) ◽  
pp. 261-261 ◽  
Author(s):  
S. S. SOMMER ◽  
G. SARKAR
Keyword(s):  
Gene Expression ◽  
Ectopic Gene Expression

scholarly journals Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells

2013 ◽  
Vol 17 (1) ◽  
pp. 23 ◽  
Author(s):  
Woosuk Kim ◽  
Ji Hyeon Kim ◽  
Sun-Young Kong ◽  
Min-Hye Park ◽  
Uy Dong Sohn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Ectopic Gene Expression

scholarly journals Ectopic gene expression and homeotic transformations in arthropods using recombinant Sindbis viruses

1999 ◽  
Vol 9 (22) ◽  
pp. 1279-1287 ◽  
Author(s):  
David L. Lewis ◽  
Mark A. DeCamillis ◽  
Craig R. Brunetti ◽  
Georg Halder ◽  
Victoria A. Kassner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ectopic Gene Expression

scholarly journals Aire unleashes stalled RNA polymerase to induce ectopic gene expression in thymic epithelial cells

2011 ◽  
Vol 109 (2) ◽  
pp. 535-540 ◽  
Author(s):  
M. Giraud ◽  
H. Yoshida ◽  
J. Abramson ◽  
P. B. Rahl ◽  
R. A. Young ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Rna Polymerase ◽  
Thymic Epithelial Cells ◽  
Ectopic Gene Expression
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