scholarly journals RBL cells as models for in vitro studies of mast cells and basophils

2018 ◽  
Vol 282 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Franco H. Falcone ◽  
Daniel Wan ◽  
Nafal Barwary ◽  
Ronit Sagi-Eisenberg
Keyword(s):  
Mast Cells ◽  
In Vitro Studies ◽  
Rbl Cells

scholarly journals Serotonin storage pools in basophil leukemia and mast cells: characterization of two types of serotonin binding protein and radioautographic analysis of the intracellular distribution of [3H]serotonin.

1982 ◽  
Vol 93 (3) ◽  
pp. 638-647 ◽  
Author(s):  
H Tamir ◽  
T C Theoharides ◽  
M D Gershon ◽  
P W Askenase
Keyword(s):  
Mast Cells ◽  
Storage Proteins ◽  
Binding Protein ◽  
Protein S ◽  
Intracellular Distribution ◽  
Con A ◽  
Intracellular Storage ◽  
Rbl Cells

We studied binding of serotonin to protein(s) derived from rat basophil leukemia (RBL) cells and mast cells. We found two types of serotonin binding protein in RBL cells. These proteins differed from one another in molecular weight and eluted in separate peaks from sephadex G-200 columns. Peak I protein (KD = 1.9 X 10(-6) M) was a glycoprotein that bound to concanavalin A (Con A); Peak II protein (KD1 = 4.5 X 10(-8) M; KD2 = 3.9 X 10(-6) M) did not bind to Con A. Moreover, binding of [3H]serotonin to protein of peak I was sensitive to inhibition by reserpine, while binding of [3H]serotonin to protein of peak II resisted inhibition by that drug. Other differences between the two types of binding protein were found, the most significant of which was the far more vigorous conditions of homogenization required to extract peak I than peak II protein. Neither peak I nor peak II protein resembled the serotonin binding protein (SBP) that is found in serotonergic neurons of the brain and gut. Electron microscope radioautographic analysis of the intracellular distribution of [3H]serotonin taken up in vitro by RBL cells or in vivo by murine mast cells indicated that essentially all of the labeled amine was located in cytoplasmic granules. No evidence for a pool in the cytosol was found and all granules were capable of becoming labeled. The presence of two types of intracellular serotonin binding proteins in these cells may indicate that there are two intracellular storage compartments for the amine. Both may be intragranular, but peak I protein may be associated with the granular membrane while peak II protein may be more free within the granular core. Different storage proteins may help to explain the differential release of amines from mast cell granules.

In-vitro Studies on the Development of Rat Peritoneal Mast Cells

Immunobiology ◽  
1980 ◽  
Vol 156 (4-5) ◽  
pp. 470-476 ◽  
Author(s):  
Beate M. Czarnetzki ◽  
Dagmar Hannich ◽  
Helmut Niedorf
Keyword(s):  
Mast Cells ◽  
In Vitro Studies ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

scholarly journals THE MORPHOLOGY AND BEHAVIOR OF NEOPLASTIC MAST CELLS CULTIVATED IN VITRO

1947 ◽  
Vol 86 (2) ◽  
pp. 117-124 ◽  
Author(s):  
George H. Paff ◽  
Frank Bloom ◽  
Christopher Reilly
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
In Vitro Studies ◽  
Hanging Drop ◽  
Cytoplasmic Granules ◽  
Original Tumor ◽  
Mast Cell Tumors ◽  
Size Number ◽  
And Behavior

Fragments from two mast cell tumors of the dog have been cultured in vitro. Studies on the living and on fixed and stained preparations revealed the following: Only mast cells grew out from the original tumor fragments though these contained other types of cells. They grew in some of the roller tube cultures in a sheet resembling an epithelium but in hanging drop cultures they lay separate and were irregularly spindle or star-shaped with long protoplasmic processes. The cytoplasmic granules of the proliferating mast cells varied in size, number, and tinctorial properties. In most of the cells they stained metachromatically, in occasional cells some of the granules only could be stained, and in a few none could be stained.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production
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