scholarly journals P9: SIMULTANEOUS DETECTION OF MAJOR FOOD ALLERGENS USING FLUORESCENT MULTIPLEX ARRAY

2018 ◽  
Vol 48 ◽  
pp. 7-7
Keyword(s):  
Simultaneous Detection ◽  
Food Allergens ◽  
Major Food ◽  
Multiplex Array

Simultaneous Detection of Four Major Food Allergens Using a Multiplex Array

2018 ◽  
Vol 141 (2) ◽  
pp. AB255
Author(s):  
Stephanie Filep ◽  
Bryan Smith ◽  
Kristina Reid Black ◽  
Martin D. Chapman
Keyword(s):  
Simultaneous Detection ◽  
Food Allergens ◽  
Major Food ◽  
Multiplex Array

scholarly journals Simultaneous Quantification of Major Food Allergens Using Fluorescent Multiplex Array

2019 ◽  
Vol 143 (2) ◽  
pp. AB240
Author(s):  
Kristina Reid Black ◽  
Stephanie Filep ◽  
Bryan R.E. Smith ◽  
Jessica Lee ◽  
Brian Murphy ◽  
...  
Keyword(s):  
Food Allergens ◽  
Simultaneous Quantification ◽  
Major Food ◽  
Multiplex Array

A Review on Identified Major Food Allergens: Characteristics and Role in Food Allergy

2017 ◽  
Vol 54 (3) ◽  
pp. 346
Author(s):  
Chhavi Arya ◽  
Chetna Jantwal
Keyword(s):  
Food Allergy ◽  
Food Allergies ◽  
Allergic Reactions ◽  
Food Allergens ◽  
Food Groups ◽  
Consumer Protection Act ◽  
Major Food ◽  
Life Threatening ◽  
Major Allergens ◽  
Allergen Labeling

Food allergens are the substances present in food that cause food allergy. Human body reactions to food allergens range from mild to severe life threatening anaphylactic shock. At least seventy different foods have been reported to cause allergic reactions and several other foods have been identified which have the potential to provoke allergic reactions. Majority of the identified food allergens are proteins. The Food Allergen Labeling and Consumer Protection Act (FALCPA) identifies eight major food groups i.e. milk, eggs, fish, crustacean shellfish, tree nuts, peanuts, wheat, and soybeans as major allergy causing foods. These eight foods are believed to account for 90 per cent of food allergies and are responsible for most serious reactions to foods. Several studies have been done which identify the major allergens in various foods. The present paper attempts to review the major allergens present in various food.

scholarly journals Food allergy and anaphylaxis – 2056. Clinical cross-reactivity of major food allergens among children

2013 ◽  
Vol 6 ◽  
pp. P139
Author(s):  
Miho Hasegawa ◽  
Takatsugu Komata ◽  
Kiyotake Ogura ◽  
Katsuhito Iikura ◽  
Sakura Sato ◽  
...  
Keyword(s):  
Food Allergy ◽  
Cross Reactivity ◽  
Food Allergens ◽  
Major Food

scholarly journals Murine IgE and IgG antibody responses to peanuts and shrimp allergens: Major food allergens in man

2003 ◽  
Vol 111 (2) ◽  
pp. S247 ◽  
Author(s):  
S.B. Lehrer ◽  
J. Fernandes ◽  
R. Reisch ◽  
P. Gaudry ◽  
G. Dai ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Food Allergens ◽  
Igg Antibody ◽  
Major Food

scholarly journals Multi-laboratory validation of the xMAP—Food Allergen Detection Assay: A multiplex, antibody-based assay for the simultaneous detection of food allergens

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0234899
Author(s):  
Eric A. E. Garber ◽  
Chung Y. Cho ◽  
Prasad Rallabhandi ◽  
William L. Nowatzke ◽  
Kerry G. Oliver ◽  
...  
Keyword(s):  
Food Allergen ◽  
Simultaneous Detection ◽  
Food Allergens ◽  
Detection Assay ◽  
Allergen Detection

Green tea catechins of food supplements facilitate pepsin digestion of major food allergens, but hampers their digestion if oxidized by phenol oxidase

2012 ◽  
Vol 4 (3) ◽  
pp. 650-660 ◽  
Author(s):  
Ziyad Tantoush ◽  
Danijela Apostolovic ◽  
Bojana Kravic ◽  
Ivana Prodic ◽  
Luka Mihajlovic ◽  
...  
Keyword(s):  
Green Tea ◽  
Phenol Oxidase ◽  
Food Supplements ◽  
Food Allergens ◽  
Tea Catechins ◽  
Pepsin Digestion ◽  
Green Tea Catechins ◽  
Major Food

Robustness Testing of the xMAP Food Allergen Detection Assay: A Multiplex Assay for the Simultaneous Detection of Food Allergens

2020 ◽  
Vol 83 (6) ◽  
pp. 1050-1056 ◽  
Author(s):  
PRASAD RALLABHANDI ◽  
CHUNG Y. CHO ◽  
WILLIAM L. NOWATZKE ◽  
KERRY G. OLIVER ◽  
ERIC A. E. GARBER
Keyword(s):  
Food Allergen ◽  
Incubation Temperature ◽  
Simultaneous Detection ◽  
Multiplex Assay ◽  
Food Allergens ◽  
Assay Procedure ◽  
Extraction Buffer ◽  
Detection Assay ◽  
Assay Performance ◽  
Allergen Detection

ABSTRACT The xMAP food allergen detection assay (xMAP FADA) can simultaneously detect 15 analytes (14 food allergens plus gluten) in one analysis. The xMAP FADA typically employs two antibody bead sets per analyte, providing built-in confirmation that is not available with other antibody-based assays. Before an analytical method can be used, its reliability must be assessed when conditions of the assay procedure are altered. This study was conducted to determine the effects on assay performance associated with changes in incubation temperature, amounts of the antibody bead cocktail, and concentrations of detection antibody and β-mercaptoethanol in the reduced-denatured extraction buffer. The analysis of buffered-detergent extracts revealed lower responses at 22°C than at 37°C, but temperature had no effect on the analysis of reduced-denatured extracts. Changes in β-mercaptoethanol and detection antibody concentrations had an effect on the detection of only milk in the reduced-denatured extracts. A slight change in the measured bead count was observed when one-fourth of the bead cocktail was used, and a large decrease in the bead count was noted when one-eighth of the recommended amount was used, but this number (≥25) was still sufficient to provide reliable results. Overall, the xMAP FADA was very robust to changes in the assay procedure, which may inadvertently occur. HIGHLIGHTS

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