Amsp3 may act upstream of Amdnmt3 in female caste differentiation in the honeybee ( Apis mellifera )

2021 ◽  
Author(s):  
L.‐X. Pan ◽  
M. Li ◽  
F.‐Y. Zhao ◽  
F.‐P. Cheng ◽  
Z.‐L. Wang
Keyword(s):  
Apis Mellifera ◽  
Caste Differentiation

scholarly journals Honeybee (Apis mellifera) Maternal Effect Causes Alternation of DNA Methylation Regulating Queen Development

Sociobiology ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 5935
Author(s):  
Xu Jiang He ◽  
Hao Wei ◽  
Wu Jun Jiang ◽  
Yi Bo Liu ◽  
Xiao Bo Wu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Apis Mellifera ◽  
Signaling Pathways ◽  
Maternal Effect ◽  
Caste Differentiation ◽  
Hippo Signaling ◽  
Thorax Length ◽  
Queen Cell ◽  
Epigenetic Analysis ◽  
Worker Caste

Queen-worker caste dimorphism is a typical trait for honeybees (Apis mellifera). We previously showed a maternal effect on caste differentiation and queen development, where queens emerged from queen-cell eggs (QE) had higher quality than queens developed from worker cell eggs (WE). In this study, newly-emerged queens were reared from QE, WE, and 2-day worker larvae (2L). The thorax size and DNA methylation levels of queens were measured. We found that queens emerging from QE had significantly larger thorax length and width than WE and 2L. Epigenetic analysis showed that QE/2L comparison had the most different methylated genes (DMGs, 612) followed by WE/2L (473), and QE/WE (371). Interestingly, a great number of DMGs (42) were in genes belonging to mTOR, MAPK, Wnt, Notch, Hedgehog, FoxO, and Hippo signaling pathways that are involved in regulating caste differentiation, reproduction and longevity. This study proved that honeybee maternal effect causes epigenetic alteration regulating caste differentiation and queen development.

scholarly journals Female developmental environment delays development of male honeybee (Apis mellifera)

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yi Bo Liu ◽  
Yao Yi ◽  
Amal Abdelmawla ◽  
Yun Lin Zheng ◽  
Zhi Jiang Zeng ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Larval Stage ◽  
Sex Differentiation ◽  
Mammalian Target Of Rapamycin ◽  
Reproductive Organs ◽  
Caste Differentiation ◽  
Whole Body ◽  
Male Development ◽  
Reproductive Tissues ◽  
Body Sizes

Abstract Background Nutrition and cell size play an important role in the determination of caste differentiation in queen and worker of honeybees (Apis mellifera), whereas the haploid genome dominates the differentiation of drones. However, the effects of female developmental environment on the development of males remain unclear. In this study, young drone larvae were transferred into worker cells (WCs) or remained in drone cells (DCs) to rear drones. The drone larvae were also grafted into queen cells (QCs) for 48 h and then transplanted into drone cells until emerging. Morphological indexes and reproductive organs of these three types of newly emerged drones were measured. Newly emerged drones and third instar drone larvae from WCs, DCs and QCs were sequenced by RNA sequencing (RNA-Seq). Results The amount of food remaining in cells of the QC and WC groups was significantly different to that in the DC group at the early larval stage. Morphological results showed that newly emerged DC drones had bigger body sizes and more well-developed reproductive tissues than WC and QC drones, whereas the reproductive tissues of QC drones were larger than those of WC drones. Additionally, whole body gene expression results showed a clear difference among three groups. At larval stage there were 889, 1761 and 1927 significantly differentially expressed genes (DEGs) in WC/DC, QC/DC and WC/QC comparisons, respectively. The number of DEGs decreased in adult drones of these three comparisons [678 (WC/DC), 338 (QC/DC) and 518 (WC/QC)]. A high number of DEGs were involved in sex differentiation, growth, olfaction, vision, mammalian target of rapamycin (mTOR), Wnt signaling pathways, and other processes. Conclusions This study demonstrated that the developmental environment of honeybee females can delay male development, which may serve as a model for understanding the regulation of sex differentiation and male development in social insects by environmental factors.

scholarly journals Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera

2007 ◽  
Vol 7 (1) ◽  
pp. 70 ◽  
Author(s):  
Angel R Barchuk ◽  
Alexandre S Cristino ◽  
Robert Kucharski ◽  
Luciano F Costa ◽  
Zilá LP Simões ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Caste Differentiation ◽  
Molecular Determinants

scholarly journals Differential Protein Expression in Honeybee (Apis mellifera L.) Larvae: Underlying Caste Differentiation

PLoS ONE ◽  
2010 ◽  
Vol 5 (10) ◽  
pp. e13455 ◽  
Author(s):  
Jianke Li ◽  
Jing Wu ◽  
Desalegn Begna Rundassa ◽  
Feifei Song ◽  
Aijuan Zheng ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Protein Expression ◽  
Caste Differentiation ◽  
Differential Protein Expression ◽  
Differential Protein
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