scholarly journals Silencing cathepsin L expression reduces Myzus persicae protein content and the nutritional value as prey for Coccinella septempunctata

2019 ◽  
Vol 28 (6) ◽  
pp. 785-797 ◽  
Author(s):  
I. Rauf ◽  
M. Asif ◽  
I. Amin ◽  
R. Z. Naqvi ◽  
N. Umer ◽  
...  
2018 ◽  
Author(s):  
Imran Rauf ◽  
Muhammad Asif ◽  
Imran Amin ◽  
Rubab Zahra Naqvi ◽  
Noroza Umer ◽  
...  

AbstractGut-expressed aphid genes, which may be more easily inhibited by RNA interference (RNAi) constructs, are attractive targets for pest control efforts involving transgenic plants. Here we show that expression of cathepsin L, a cysteine protease that functions in aphid guts, can be reduced by expression of an RNAi construct in transgenic tobacco. The effectiveness of this approach is demonstrated by up to 80% adult mortality, reduced fecundity, and delayed nymph production of Myzus persicae (green peach aphids) when cathepsin L expression was reduced by plant-mediated RNAi. Consistent with the function of cathepsin L as a gut protease, M. persicae fed on the RNAi plants had a lower protein content in their bodies and excreted more protein in their honeydew. Larvae of Coccinella septempunctata (seven-spotted ladybugs) grew more slowly on aphids having reduced cathepsin L expression, suggesting that prey insect nutritive value, and not just direct negative effects of the RNAi construct, needs to be considered when producing transgenic plants for RNAi-mediated pest control.HighlightsSilencing expression of cathepsin L by RNA interference reduces protein content of Myzus persicae (green peach aphid) bodies.Honeydew of aphids with cathepsin L silenced contains elevated protein.Cathepsin L is required for efficient protein uptake from phloem sap.Aphids with cathepsin L expression silenced have increased mortality and fewer offspring.Coccinella septempunctata (seven-spotted ladybugs) grow more slowly on aphids with expression of cathepsin L silenced.


Author(s):  
Antonia Odagiu ◽  
I. Oroian ◽  
Tania Mihăiescu

The identification of the suitable sample size and adequate sampling place when alfalfa hay was chemically analyzed in order to determine its nutritional value was the aim of our test. Two loads of alfalfa hay were used for collecting samples, two sizes each (15 and 10 subsamples, variant I and II, respectively). The crude protein content was determined using AOAC protocol. By assessing the statistic parameters of the crude protein content of the forage, function of the sampling place and sample size, we observed the influence of the sampling technique on the accuracy of the determinations. The highest coefficient of variability was recorded when samples were collected from the base of the load in each case. Only the crude protein content differences between the samples collected from the other parts of the load and base of the load (variant I, in both loads) were statistically significant (p < 0.05). Within the same load, no significant differences (p > 0.05) were recorded. Even notable differences were not obtained in crude protein content determination function of sampling place or sampling size, the need and care in sampling should be considered.


2021 ◽  
Vol 6 (3) ◽  
pp. 455-464
Author(s):  
Emma Suri Yanti Siregar ◽  
Tengku Muhammad Ghazali ◽  
Rosmasita Rosmasita ◽  
Dian Fitria ◽  
Juliana Pebrina Siburian ◽  
...  

 Indonesia is a maritime country that has many marine natural resources. Central Tapanuli Regency has a position close to the sea. Even though fish has a high protein content with amino acids, students or young people still like to eat fish a little. So it is necessary to provide information and at the same time invite the younger generation to like to eat fish to increase their intelligence. This community service activity was carried out at MTS Al-Maidar Pandan, Pandan District, Central Tapanuli Regency. The method of implementing this service activity is done by providing socialization about the importance of eating fish for MTS Al Maidar students. The outcomes achieved are increasing students' knowledge about the benefits of fish consumption and the nutritional value contained in fish as well as providing students' understanding with the knowledge to be able to distinguish between fish that are suitable for daily consumption or not.


2012 ◽  
Vol 48 (3) ◽  
pp. 414-427 ◽  
Author(s):  
LIKAWENT YEHEYIS ◽  
C. KIJORA ◽  
E. VAN SANTEN ◽  
M. WINK ◽  
JÜRGEN DANIER ◽  
...  

SUMMARYThough bitter white lupin (Lupinus albus L.) is a traditional crop in Ethiopia, sweet lupins are new to the country. As a result, the nutritional value of low-alkaloid lupins has not been evaluated under Ethiopian conditions. Crude protein, amino acid and alkaloid contents of 16 cultivars of three annual lupin species grown in four lupin growing locations (Merawi, Finoteselam, Kossober-1 and Kossober-2) of Ethiopia were evaluated. Location × cultivar interaction was a significant source of variation for all traits (p < 0.0001). In all locations, blue entries had either similar (p ≥ 0.0584) or higher (p ≤ 0.0235) forage crude protein content than the Local Landrace, white group and yellow entry. Compared with the Local Landrace, white and blue entries, the sole yellow entry had higher (p ≤ 0.0148) seed crude protein content at all locations except at Kossober-2, where it had similar (p = 0.8460) crude protein content as white entries. The Local Landrace had the highest forage and seed alkaloid contents. However, sweet blue Vitabor and Sanabor entries had the lowest forage and seed alkaloid contents, respectively. Low alkaloid and higher crude protein contents of sweet lupins grown in Ethiopia show the possibility to use sweet lupin forage and seeds as cheap home-grown protein source for livestock feed and human food in the country. However, for more reliable information, the laboratory results need to be verified by animal and human evaluations of the crop.


Author(s):  
Р.Х. КАНДРОКОВ ◽  
С.Е. ТЕРЕНТЬЕВ ◽  
Н.В. ЛАБУТИНА ◽  
М.Ш. БЕГЕУЛОВ ◽  
И.Г. БЕЛЯВСКАЯ ◽  
...  

В России наблюдается повышенный интерес к продуктам питания, включающим нетрадиционное сырье. Спрос на продукты с добавкой семян конопли (Cannabis sativa L.) обусловлен их питательной ценностью и низкой аллергенностью. Исследованы химические и физико-химические показатели пшенично-конопляной муки, полученной из помольной смеси зерна яровой пшеницы сорта Радмира и семян конопли сорта Сурская в соотношении соответственно 95 : 5, 92,5 : 7,5 и 90 : 10%. В качестве контрольного образца была пшеничная мука без добавок. Размол помольных пшенично-конопляных смесей различного соотношения и зерна пшеницы проводили на мельницах лабораторного помола (МЛП-4) с нарезными (драные системы) и микрошероховатыми вальцами (размольные системы). Исследование химических и физико-химических свойств образцов пшенично-конопляной и пшеничной муки проводили на инфракрасном анализаторе SpectraStar 2500 XL. Установлено, что добавление семян конопли в помольную смесь существенно снижает выход пшенично-конопляной муки по сравнению с выходом пшеничной муки (при добавке 5–7,5% семян конопли выход снижается на 4,3–4,4%, а при добавке 10% семян конопли – на 10,6%), однако повышается содержание жира и белка во всех потоках пшенично-конопляной муки, полученной как с драных, так и с размольных систем. По сравнению с содержанием жира и белка в муке из зерна пшеницы, составившим 1,12 и 11,57% соответственно, при добавлении 5% семян конопли в помольную пшенично-конопляную зерновую смесь средневзвешенное содержание жира в пшенично-конопляной муке составило 3,76%, а содержание белка – 12,74%, при добавлении 7,5% конопли в помольную смесь средневзвешенное содержание жира в пшенично-конопляной муке составило 4,35%, а белка – 12,7%, при добавлении 10% конопли в помольную смесь средневзвешенное содержание жира в пшенично-конопляной муке составило 4,97%, а содержание белка – 12,4%. Повышение содержания жира и белка в пшенично-конопляной муке будет способствовать повышению пищевой ценности хлебобулочного изделия из нее. Increased interest in food products, including non-traditional raw materials, is observe among the population of Russia. The demand for products with the addition of hemp seeds (Cannabis sativa L.) is due to their nutritional value and low allergenicity. Chemical and physico-chemical parameters of wheat-hemp flour obtained from a grind mixture of Radmira spring wheat grain and Surskaya hemp seeds in the ratio: 95 : 5, 92,5 : 7,5 and 90 : 10%, respectively, were investigated. Wheat flour without additives was use as a control sample. Grinding of grind wheat-hemp mixtures of various ratios and wheat grains was carried out in laboratory grind mills with rifled (torn systems) and micro-roughened rollers (grinding systems). The study of chemical and physico-chemical properties of wheat-hemp and wheat flour samples was carried out on the SpectraStar 2500 XL infrared analyzer. It was found that the addition of hemp seeds to the grind mixture significantly reduces the yield of wheat-hemp flour compared with the yield of wheat flour (with the addition of 5–7,5% of hemp seeds, the yield decreases by 4,3–4.4%, and with the addition of 10% of hemp seeds – by 10,6%), however increases the fat and protein content in all streams of wheat-hemp flour obtained from both torn and grinding systems. Compared with the fat and protein content in wheat flour, which amounted to 1,12 and 11,57% respectively, when adding 5% hemp to the wheat-hemp grain mixture, the weighted average fat content in wheat-hemp flour was 3,76%, and the protein content was 12,74%, when adding 7,5% hemp to the grind mixture, the weighted average fat content in wheat-hemp flour was 4,35%, and protein – 12,7%, and when adding 10% the weighted average fat content in wheat-hemp flour in the grind mixture was 4,97%, and the protein content was 12,4%. An increase in the fat and protein content in wheat and hemp flour will contribute to an increase in the nutritional value of a bakery product made from it.


2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Qiannan Yu ◽  
Meijuan Guo ◽  
Bin Zhang ◽  
Hao Wu ◽  
Yan Zhang ◽  
...  

To explore the differences in the main nutritional composition of 23 kinds of common edible fungi in the market, the moisture, ash, protein, fat, dietary fiber, carbohydrates, polysaccharides, and energy were analyzed using national standard methods. The results showed that the 23 kinds of edible fungi varied greatly in nutritional composition. Based on dry weight, the moisture content was 6.9∼15.5 g/100 g, the ash content ranged from 1.3 to 10.1 g/100 g, the protein content ranged from 8.5 to 36.9 g/100 g, the fat content was 0.5∼3.9 g/100 g, the dietary fiber content was between 14.4∼70.2 g/100 g, the carbohydrate content is 0.5∼37.3 g/100 g, the polysaccharide content was 2.1∼8.3 g/100 g, and the energy is about 751∼1322 100 g/kJ. All the 23 kinds of edible fungi can be regarded as high-protein low-fat foods, which have their own advantages in terms of nutritional value. This study provides reference for people to use edible fungi in a more scientific and reasonable way.


1997 ◽  
Vol 53 (1-3) ◽  
pp. 31-45 ◽  
Author(s):  
Marcello Duranti ◽  
Cristina Gius

Author(s):  
Satvir Kaur ◽  
Sarbjit Kaur ◽  
Anil K Gupta ◽  
Jagmeet Kaur

The present investigation aims to analyze nutrient profile, antinutritional traits and physiochemical characteristics in raw (dry seeds) and water soaked seeds of eighteen chickpea genotypes comprising 16 cold stress tolerant and 2 susceptible. Raw seeds of tolerant genotypes were found to have higher total sugars and protein content and lower amount of trypsin inhibitors. Higher content of raffinose family oligosaccharides in tolerant genotypes is an additional characteristic for their tolerant behaviour and it does not interfere with the digestion as with soaking these oligosaccharides are reduced by 87%. The enhanced mineral content with soaking in tolerant chickpea genotypes might be attributed to the better physiochemical characteristics in terms of higher hydration capacity and swelling index which leads to greater permeable and softer cotyledons that might help in leaching out of mineral ligands. The higher nutritional value along with better physiochemical characteristics observed in tolerant genotypes render them useful at consumer level as well as from breeding point of view.


BioControl ◽  
2005 ◽  
Vol 50 (4) ◽  
pp. 565-588 ◽  
Author(s):  
Ioanna Katsarou ◽  
John T. Margaritopoulos ◽  
John A. Tsitsipis ◽  
Dionyssios Ch. Perdikis ◽  
Kostas D. Zarpas

Author(s):  
Bulkaini Bulkaini ◽  
Djoko Kisworo ◽  
Sukirno Sukirno ◽  
Rani Wulandani ◽  
Maskur Maskur

The study was aimed to determine the effect of tapioca flour addition atvarious levels on the quality of chicken sausage. The research was design based on a Completely Randomized Design (CRD) with 4 treatments and 3 replications. The treatment of tapioca flour addition level were: 0%, 10%, 20% and 30%. Data ofsausage quality were analyzed using Analysis of Variance and continued with Duncan's Multiple Range Test. The results showed that the addition of tapioca flour in the process of chicken sausage making were significantly affect (P <0.05) moisture and protein content, while the fat and ash content were not significantly effected (P> 0.05) by the treatment. The addition of 10% tapioca flour obtained the highest nutritional value of protein (17.67 ± 0.93) % as compared to the other levels.


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