scholarly journals Suppressor of hairy‐wing, modifier of mdg4 and centrosomal protein of 190 gene orthologues of the gypsy insulator complex in the malaria mosquito, Anopheles stephensi

2016 ◽  
Vol 25 (4) ◽  
pp. 460-469
Author(s):  
R. Carballar‐Lejarazú ◽  
P. Brennock ◽  
A. A. James
Keyword(s):  
Anopheles Stephensi ◽  
Malaria Mosquito ◽  
Gypsy Insulator ◽  
Centrosomal Protein

scholarly journals Wild chive oil is an extremely effective larvicide against malaria mosquito vector Anopheles stephensi

2019 ◽  
Vol 12 (4) ◽  
pp. 170 ◽  
Author(s):  
Moussa Soleimani-Ahmadi ◽  
Alireza Sanei-Dehkordi ◽  
YaserSalim Abadi ◽  
Azim Paksa
Keyword(s):  
Anopheles Stephensi ◽  
Mosquito Vector ◽  
Malaria Mosquito

scholarly journals Pure early zygotic genes in the Asian malaria mosquito Anopheles stephensi

2018 ◽  
Vol 11 (S2) ◽  
Author(s):  
Yang Wu ◽  
Wanqi Hu ◽  
James K. Biedler ◽  
Xiao-Guang Chen ◽  
Zhijian Jake Tu
Keyword(s):  
Anopheles Stephensi ◽  
Malaria Mosquito

scholarly journals Cas9-Mediated Gene-Editing in the Malaria Mosquito Anopheles stephensi by ReMOT Control

2020 ◽  
Vol 10 (4) ◽  
pp. 1353-1360 ◽  
Author(s):  
Vanessa M. Macias ◽  
Sage McKeand ◽  
Duverney Chaverra-Rodriguez ◽  
Grant L. Hughes ◽  
Aniko Fazekas ◽  
...  
Keyword(s):  
Gene Editing ◽  
Molecular Mechanisms ◽  
Anopheles Stephensi ◽  
Mosquito Control ◽  
Control Strategies ◽  
Mosquito Species ◽  
Adult Mosquito ◽  
Malaria Mosquito ◽  
Powerful Method ◽  
Injection Methods

Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission of malaria parasites by Anopheles mosquitoes. CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology of vector-pathogen interactions and can itself form the basis of mosquito control strategies. However, embryo injection methods used to genetically manipulate mosquitoes (especially Anopheles) are difficult and inefficient, particularly for non-specialist laboratories. Here, we adapted the ReMOT Control (Receptor-mediated Ovary Transduction of Cargo) technique to deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector Anopheles stephensi without injecting embryos. In Anopheles, ReMOT Control gene editing was as efficient as standard embryo injections. The application of ReMOT Control to Anopheles opens the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise to perform embryo injections and establishes the flexibility of ReMOT Control for diverse mosquito species.

scholarly journals Dynamic expression of miRNAs across immature and adult stages of the malaria mosquito Anopheles stephensi

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Shanu Jain ◽  
Vandita Rana ◽  
Adak Tridibes ◽  
Sujatha Sunil ◽  
Raj K Bhatnagar
Keyword(s):  
Anopheles Stephensi ◽  
Malaria Mosquito ◽  
Dynamic Expression

Post-integration behavior of a Minos transposon in the malaria mosquito Anopheles stephensi

2007 ◽  
Vol 278 (5) ◽  
pp. 575-584 ◽  
Author(s):  
Christina Scali ◽  
Tony Nolan ◽  
Igor Sharakhov ◽  
Maria Sharakhova ◽  
Andrea Crisanti ◽  
...  
Keyword(s):  
Anopheles Stephensi ◽  
Malaria Mosquito
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