Functional characterization of calliphorid cell death genes and cellularization gene promoters for controlling gene expression and cell viability in early embryos

2014 ◽  
Vol 24 (1) ◽  
pp. 58-70 ◽  
Author(s):  
R. M. Edman ◽  
R. J. Linger ◽  
E. J. Belikoff ◽  
F. Li ◽  
S.-H. Sze ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Cell Viability ◽  
Functional Characterization ◽  
Gene Promoters ◽  
Early Embryos ◽  
Cell Death Genes ◽  
Death Genes

scholarly journals dLKR/SDH regulates hormone-mediated histone arginine methylation and transcription of cell death genes

2008 ◽  
Vol 182 (3) ◽  
pp. 481-495 ◽  
Author(s):  
Dimitrios Cakouros ◽  
Kathryn Mills ◽  
Donna Denton ◽  
Alicia Paterson ◽  
Tasman Daish ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Gene Activation ◽  
Arginine Methylation ◽  
Gene Promoters ◽  
Methyl Transferase ◽  
Saccharopine Dehydrogenase ◽  
Cell Death Genes ◽  
Regulated Gene Expression ◽  
Death Genes

The sequential modifications of histones form the basis of the histone code that translates into either gene activation or repression. Nuclear receptors recruit a cohort of histone-modifying enzymes in response to ligand binding and regulate proliferation, differentiation, and cell death. In Drosophila melanogaster, the steroid hormone ecdysone binds its heterodimeric receptor ecdysone receptor/ultraspiracle to spatiotemporally regulate the transcription of several genes. In this study, we identify a novel cofactor, Drosophila lysine ketoglutarate reductase (dLKR)/saccharopine dehydrogenase (SDH), that is involved in ecdysone-mediated transcription. dLKR/SDH binds histones H3 and H4 and suppresses ecdysone-mediated transcription of cell death genes by inhibiting histone H3R17me2 mediated by the Drosophila arginine methyl transferase CARMER. Our data suggest that the dynamic recruitment of dLKR/SDH to ecdysone-regulated gene promoters controls the timing of hormone-induced gene expression. In the absence of dLKR/SDH, histone methylation occurs prematurely, resulting in enhanced gene activation. Consistent with these observations, the loss of dLKR/SDH in Drosophila enhances hormone-regulated gene expression, affecting the developmental timing of gene activation.

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Network Analysis Identifies Drug Targets and Small Molecules to Modulate Apoptosis Resistant Cancers

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 851
Author(s):  
Samreen Fathima ◽  
Swati Sinha ◽  
Sainitin Donakonda
Keyword(s):  
Cell Death ◽  
Small Molecules ◽  
Drug Targets ◽  
Apoptotic Cell ◽  
Colon Adenocarcinoma ◽  
Interaction Networks ◽  
Small Cell Lung ◽  
Protein Protein Interaction ◽  
Cell Death Genes ◽  
Death Genes

Programed cell death or apoptosis fails to induce cell death in many recalcitrant cancers. Thus, there is an emerging need to activate the alternate cell death pathways in such cancers. In this study, we analyzed the apoptosis-resistant colon adenocarcinoma, glioblastoma multiforme, and small cell lung cancers transcriptome profiles. We extracted clusters of non-apoptotic cell death genes from each cancer to understand functional networks affected by these genes and their role in the induction of cell death when apoptosis fails. We identified transcription factors regulating cell death genes and protein–protein interaction networks to understand their role in regulating cell death mechanisms. Topological analysis of networks yielded FANCD2 (ferroptosis, negative regulator, down), NCOA4 (ferroptosis, up), IKBKB (alkaliptosis, down), and RHOA (entotic cell death, down) as potential drug targets in colon adenocarcinoma, glioblastoma multiforme, small cell lung cancer phenotypes respectively. We also assessed the miRNA association with the drug targets. We identified tumor growth-related interacting partners based on the pathway information of drug-target interaction networks. The protein–protein interaction binding site between the drug targets and their interacting proteins provided an opportunity to identify small molecules that can modulate the activity of functional cell death interactions in each cancer. Overall, our systematic screening of non-apoptotic cell death-related genes uncovered targets helpful for cancer therapy.

scholarly journals 1779 Cell Death Genes are Induced Immediately after Hypoxia-Reoxygenation (HR) in the Newborn Mouse Lung

2012 ◽  
Vol 97 (Suppl 2) ◽  
pp. A503-A503
Author(s):  
E. Wollen ◽  
A. Rognlien ◽  
M. Atneosen-Asegg ◽  
M. Wright ◽  
M. Bjoras ◽  
...  
Keyword(s):  
Cell Death ◽  
Mouse Lung ◽  
Newborn Mouse ◽  
Cell Death Genes ◽  
Death Genes ◽  
Hypoxia Reoxygenation

Interleukin-1α-converting enzyme (ICE) and related cell death genes ICErel-II and ICErel-III map to the same PAC clone at band 11q22.2-22.3

1997 ◽  
Vol 8 (8) ◽  
pp. 611-613 ◽  
Author(s):  
Jamal Nasir ◽  
Jane L. Theilmann ◽  
John P. Vaillancourt ◽  
Neil A. Munday ◽  
Ambereen Ali ◽  
...  
Keyword(s):  
Cell Death ◽  
Converting Enzyme ◽  
Related Cell ◽  
Interleukin 1Α ◽  
Cell Death Genes ◽  
Death Genes
Sign in / Sign up

Export Citation Format

Share Document