Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1linked with insecticide resistance in the brown planthopper,Nilaparvata lugens

2014 ◽  
Vol 23 (6) ◽  
pp. 768-778 ◽  
Author(s):  
R. Pang ◽  
Y. Li ◽  
Y. Dong ◽  
Z. Liang ◽  
Y. Zhang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Insecticide Resistance ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Promoter Polymorphisms

Purification, molecular cloning and heterologous expression of a glutathione S-transferase involved in insecticide resistance from the rice brown planthopper, Nilaparvata lugens

2002 ◽  
Vol 362 (2) ◽  
pp. 329-337 ◽  
Author(s):  
John G. VONTAS ◽  
Graham J. SMALL ◽  
Dimitra C. NIKOU ◽  
Hilary RANSON ◽  
Janet HEMINGWAY
Keyword(s):  
Insecticide Resistance ◽  
Peroxidase Activity ◽  
Pyrethroid Resistance ◽  
Brown Planthopper ◽  
Resistance Mechanism ◽  
Amino Acid Identity ◽  
Nilaparvata Lugens ◽  
Northern Analysis ◽  
Affinity Column ◽  
Glutathione S Transferase

A novel glutathione S-transferase (GST)-based pyrethroid resistance mechanism was recently identified in Nilaparvata lugens [Vontas, Small and Hemingway (2001) Biochem. J. 357, 65–72]. To determine the nature of GSTs involved in conferring this resistance, the GSTs from resistant and susceptible strains of N. lugens were partially purified by anion exchange and affinity chromatography. The majority of peroxidase activity, previously correlated with resistance, was confined to the fraction that bound to the affinity column, which was considerably elevated in the resistant insects. A cDNA clone encoding a GST (nlgst1-1) —the first reported GST sequence from Hemiptera with up to 54% deduced amino-acid identity with other insect class I GSTs—was isolated from a pyrethroid-resistant strain. Northern analysis showed that nlgst1-1 was overexpressed in resistant insects. nlgst1-1 was expressed in Escherichia coli, purified and characterized. The ability of the recombinant protein to bind to the S-hexylglutathione affinity matrix, its substrate specificities and its immunological properties confirmed that this GST was one from the elevated subset of N. lugens GSTs. Peroxidase activity of the recombinant nlgst1-1 indicated that it had a role in resistance, through detoxification of lipid peroxidation products induced by pyrethroids. Southern analysis of genomic DNA from the resistant and susceptible strains indicated that GST-based insecticide resistance may be associated with gene amplification in N. lugens.

Adipokinetic hormone regulates cytochrome P450-mediated imidacloprid resistance in the brown planthopper, Nilaparvata lugens

Chemosphere ◽  
2020 ◽  
Vol 259 ◽  
pp. 127490 ◽  
Author(s):  
Bingjie Tang ◽  
Yibei Cheng ◽  
Yimin Li ◽  
Wenru Li ◽  
Ying Ma ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Adipokinetic Hormone
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