The antenna-specific odorant-binding protein AlinOBP13 of the alfalfa plant bugAdelphocoris lineolatusis expressed specifically in basiconic sensilla and has high binding affinity to terpenoids

2014 ◽  
Vol 23 (4) ◽  
pp. 417-434 ◽  
Author(s):  
L. Sun ◽  
H-J. Xiao ◽  
S-H. Gu ◽  
J-J. Zhou ◽  
Y-Y. Guo ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Alfalfa Plant ◽  
Basiconic Sensilla ◽  
High Binding ◽  
Odorant Binding ◽  
High Binding Affinity

Vitamin B12 radioassay with oyster toadfish (Opsanus tau) serum as binder.

1977 ◽  
Vol 23 (6) ◽  
pp. 1037-1045 ◽  
Author(s):  
D O Kubiatowicz ◽  
D S Ithakissios ◽  
D C Windorski
Keyword(s):  
Vitamin B12 ◽  
Binding Affinity ◽  
Incubation Temperature ◽  
Binding Protein ◽  
High Capacity ◽  
Opsanus Tau ◽  
High Binding ◽  
Denatured Proteins ◽  
Oyster Toadfish ◽  
High Binding Affinity

Abstract We describe a vitamin B12 radioassay in which oyster toadfish (Opsanus tau) serum is used as the binding protein. The serum is quite stable and has a high capacity and high binding affinity (K greater than 10(12) liter/mol) for vitamin B12. The binding is not significantly affected by temperature, the presence of denatured proteins, or the amount of vitamin B12 present. The radioassay is reproducible (CV 4.4%) within assay, 7.8% between assays) and sensitive (12 ng/liter). Assay accuracy is unaffected by the protein in serum or by reasonable variations in incubation temperature and time.

Rapid conversion of IgG to biosensor using an antibody-binding protein-based probe

The Analyst ◽  
2021 ◽  
Author(s):  
Guangwei Zhao ◽  
Haimei Li ◽  
Baowei Li ◽  
Mei Li ◽  
Wei Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Binding Affinity ◽  
Binding Protein ◽  
Antibody Binding ◽  
Full Length ◽  
High Binding ◽  
High Binding Affinity ◽  
Rapid Conversion

We successfully developed a fluorescent probe that can quickly convert full-length antibodies to Quenchbodies, which represent a type of fluorescent immunosensor with high binding affinity and specificity depending on the...

scholarly journals Herb-target virtual screening and network pharmacology for prediction of molecular mechanism of Danggui Beimu Kushen Wan for prostate cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hong Li ◽  
Andrew Hung ◽  
Angela Wei Hong Yang
Keyword(s):  
Prostate Cancer ◽  
Binding Affinity ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Tight Binding ◽  
Experimental Studies ◽  
Network Pharmacology ◽  
High Morbidity ◽  
High Binding ◽  
High Binding Affinity

AbstractProstate cancer (PCa) is a cancer that occurs in the prostate with high morbidity and mortality. Danggui Beimu Kushen Wan (DBKW) is a classic formula for patients with difficult urination including PCa. This study aimed to investigate the molecular mechanisms of DBKW for PCa. We obtained DBKW compounds from our previous reviews. We identified potential targets for PCa from literature search, currently approved drugs and Open Targets database and filtered them by protein–protein interaction network analysis. We selected 26 targets to predict three cancer-related pathways. A total of 621 compounds were screened via molecular docking using PyRx and AutoDock Vina against 21 targets for PCa, producing 13041 docking results. The binding patterns and positions showed that a relatively small number of tight-binding compounds from DBKW were predicted to interact strongly and selectively with three targets. The top five high-binding-affinity compounds were selected to generate a network, indicating that compounds from all three herbs had high binding affinity against the 21 targets and may have potential biological activities with the targets. DBKW contains multi-targeting agents that could act on more than one pathway of PCa simultaneously. Further studies could focus on validating the computational results via experimental studies.

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