Prospective evaluation of two specific IgG immunoassays (HemosIL ® AcuStar HIT‐IgG and HAT45G ® ) for the diagnosis of heparin‐induced thrombocytopenia: A Bayesian approach

2020 ◽  
Author(s):  
Emilie Jousselme ◽  
Eve‐Anne Guéry ◽  
Christophe Nougier ◽  
Frédéric Sobas ◽  
Jérôme Rollin ◽  
...  
Keyword(s):  
Bayesian Approach ◽  
Prospective Evaluation ◽  
Heparin Induced Thrombocytopenia ◽  
Specific Igg

scholarly journals Prospective evaluation of PF4/heparin immunoassays for the diagnosis of heparin-induced thrombocytopenia

2009 ◽  
Vol 7 (8) ◽  
pp. 1260-1265 ◽  
Author(s):  
T. BAKCHOUL ◽  
A. GIPTNER ◽  
A. NAJAOUI ◽  
G. BEIN ◽  
S. SANTOSO ◽  
...  
Keyword(s):  
Prospective Evaluation ◽  
Heparin Induced Thrombocytopenia

Rapid and simple IgG specific test for the exclusion of heparin induced thrombocytopenia (HIT)

2011 ◽  
Vol 49 (12) ◽  
Author(s):  
Hans-Jürgen Kolde ◽  
Ralf Dostatni ◽  
Susanne Mauracher
Keyword(s):  
Anticoagulation Therapy ◽  
Clinical Samples ◽  
Functional Assay ◽  
Specific Test ◽  
Platelet Factor ◽  
Heparin Induced Thrombocytopenia ◽  
Activation Assay ◽  
Specific Igg ◽  
Positive Results ◽  
Specific Igg Antibodies

AbstractThe exclusion of heparin induced thrombocytopenia (HIT) is required for selecting the most appropriate anticoagulation therapy in affected patients. It requires the combination of clinical data with the detection of antibodies directed against platelet factor 4 (PF4) in complex with polyanions (PA) such as heparin.We developed a lateral flow immunoassay (LFIA) for PF4/PA complex specific IgG antibodies based on gold nanoparticles. Unlike most other assays, the initial immune reaction takes place in the liquid phase. The sensitivity of the assay has been adjusted with clinical samples aiming in the reliable detection of sera which are positive in a functional platelet activation assay.Sera from 60 patients with suspected HIT were investigated. LFIA identified correctly all samples (n=20) which were positive in a functional assay (HIPA) and an IgG specific ELISA. It correlated with ELISA, but false positive results were less frequent (7 samples were negative with LFIA and HIPA but positive with ELISA).The LFIA may be a suitable tool for the rapid exclusion of HIT within 10 min.

scholarly journals FcγRIIA H/R131 Polymorphism, Subclass-Specific IgG Anti-Heparin/Platelet Factor 4 Antibodies and Clinical Course in Patients With Heparin-Induced Thrombocytopenia and Thrombosis

Blood ◽  
1997 ◽  
Vol 89 (2) ◽  
pp. 370-375 ◽  
Author(s):  
Gowthami Arepally ◽  
Steven E. McKenzie ◽  
Xiao-Ming Jiang ◽  
Mortimer Poncz ◽  
Douglas B. Cines
Keyword(s):  
Amino Acid ◽  
Disease Susceptibility ◽  
Clinical Course ◽  
Platelet Factor 4 ◽  
Cross Linking ◽  
Functional Polymorphism ◽  
Platelet Factor ◽  
Heparin Induced Thrombocytopenia ◽  
Receptor Isoforms ◽  
Specific Igg

Abstract The explanation why only a subset of patients with heparin-induced thrombocytopenia (HIT) develop clinically apparent thromboses (HITT) remains uncertain. It has been proposed that platelet activation induced by cross-linking of FcγRIIA by anti-heparin/platelet factor 4 (PF4) antibodies is central to the pathogenesis of thrombosis. The observation that a common functional polymorphism of FcγRIIA, involving either an arginine (R) or histidine (H) at amino acid 131, may underlie disease susceptibility prompted us to investigate the prevalence of receptor isoforms in patients with HIT and HITT. Furthermore, because these isoforms reportedly differ in their avidity for immune complexes containing human IgG2 , we also analyzed sera from patients with HIT and HITT for the prevalence of various subclass-specific IgG anti-heparin/PF4 antibodies. No difference in the allele frequency of FcγRIIA-H131 or R131 was identified among 13 patients with HIT or 23 with HITT compared with 102 controls (χ2 = 1.21, P = .8). Furthermore, although most patients had IgG2 antibodies (62%), IgG1 was the predominant subclass in 30 of the 34 patients with IgG anti-heparin/PF4 antibodies and in 12 was the exclusive subclass found. Also, there was no association between the concordance of IgG2 anti-heparin/PF4 antibodies and the expression of FcγRIIA-H131 in patients with HITT compared with patients with thrombocytopenia alone. These results make it unlikely that the FcγRIIA-H131 isoform or IgG2 anti-heparin/PF4 antibodies are required to develop HITT, suggesting that factors in addition to cross-linking of FcγRIIA receptors contribute to the pathogenesis of thrombosis in patients with heparin-dependent antiplatelet antibodies.

scholarly journals P165 Prospective evaluation of Aspergillus fumigatus-specific IgG in patients with cystic fibrosis

2019 ◽  
Vol 18 ◽  
pp. S104
Author(s):  
P. Eschenhagen ◽  
P. Bacher ◽  
S. Kaufmann ◽  
V. Rickerts ◽  
K. Seidel ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Aspergillus Fumigatus ◽  
Prospective Evaluation ◽  
Specific Igg
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