Analysis of time and temperature stability of EDTA anticoagulation whole blood for complete blood count parameters with the use of Abbott CELL‐DYN Sapphire hematology analyzer

2019 ◽  
Vol 42 (2) ◽  
Author(s):  
Chi Zhang ◽  
Hui Zhang ◽  
Guo Li ◽  
Hongbo Zhang ◽  
Yang Fei
Keyword(s):  
Whole Blood ◽  
Blood Count ◽  
Complete Blood Count ◽  
Temperature Stability ◽  
Hematology Analyzer

scholarly journals Comparison of effect of aloe Vera gel with aspirin and celecoxib on platelet aggregation.

2020 ◽  
Vol 27 (05) ◽  
pp. 973-978
Author(s):  
Sidra Mushtaq ◽  
Zobia Mushtaq ◽  
Javeria Arif ◽  
Mufakhara Fatima ◽  
Sadida Bahawal ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Count ◽  
Complete Blood Count ◽  
Platelet Rich Plasma ◽  
Aloe Vera ◽  
Medical Institute ◽  
Aloe Vera Gel ◽  
Study Setting

Objective: This study was designed to compare the effect of Aloe vera gel with aspirin and celecoxib on platelet aggregation. Study Design: Comparative Study. Setting: Post graduate Medical Institute Lahore, Children Hospital, Lahore. Period: September 2015 to September 2016. Material & Methods: Blood was withdrawn from anti-cubital vein, complete blood count was checked, platelet rich plasma was prepared by centrifuging citrated whole blood and then incubated with  Aloe vera low (AVL), Aloe vera high (AVH), aspirin and celecoxib for 30 minutes at 37C. After adding the agonist arachidonic acid, reading was then taken for 3 minutes and percentage aggregation was recorded. Results: Platelet aggregation with aspirin, AVH and AVL was statistically significantly lower as compared to control and celecoxib groups. Conclusion: This study has demonstrateda dose dependentanti-platelet effect of Aloe vera gel which is comparable to aspirin.

On-chip sample preparation for complete blood count from raw blood

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1533-1544 ◽  
Author(s):  
John Nguyen ◽  
Yuan Wei ◽  
Yi Zheng ◽  
Chen Wang ◽  
Yu Sun
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
Whole Blood ◽  
Blood Count ◽  
Complete Blood Count ◽  
Chip Sample ◽  
On Chip

We present a monolithic microfluidic device capable of on-chip sample preparation for both RBC and WBC measurements from whole blood.

scholarly journals Comparison of Different Small Clinical Hematology Laboratory Configurations With Focus on Remote Smear Imaging

2019 ◽  
Vol 143 (10) ◽  
pp. 1234-1245 ◽  
Author(s):  
Laura Stephens ◽  
Nicholas J. Bevins ◽  
Hans-Inge Bengtsson ◽  
H. Elizabeth Broome
Keyword(s):  
Digital Imaging ◽  
Blood Count ◽  
Complete Blood Count ◽  
Cost Savings ◽  
Reference Method ◽  
Cost Modeling ◽  
Special Focus ◽  
Imaging Device ◽  
Cell Counts ◽  
Hematology Analyzer

Context.— Stand-alone clinical sites (eg, infusion centers) are becoming increasingly common. These sites require timely hematology analysis. Here we compare performance and costs of currently available analysis configurations with special focus on a proposed alternative using a minimal hematology analyzer plus a digital imaging device, allowing for remote oversight and interpretation. Objectives.— To determine whether low-volume laboratories might realize savings while gaining function by substituting commonly used configurations with a proposed alternative. Design.— To evaluate the performance of the proposed alternative configuration, blood counts with automated differentials produced by a Sysmex XE5000 (complete blood count reference method) were compared with cell counts from the Sysmex pocH-100i, CellaVision DM96 preclassified differentials, and DM96 reclassified differentials (differential reference method) by using standard regression analyses, 95% CIs, and truth tables. Financial cost modeling used staffing practices, test volumes, and smear production rates observed at remote clinics performing on-site hematology analysis within the University of California at San Diego Health system. Results.— Differential blood count parameters showed excellent correlation between the XE5000 and preclassification DM96 with R2 > 0.95. For blasts/abnormal cells, immature granulocytes, and nucleated red blood cells, the DM96 showed higher sensitivity and similar specificity to the XE5000. Cost modeling revealed that decreased personnel costs through remote monitoring of results facilitated by the DM96 would lead to lower operational costs relative to more conventional analysis configurations. Conclusions.— A digital imaging instrument with an inexpensive hematology analyzer provides similar information to a complex hematology analyzer and allows remote review of the blood smear findings by experts, leading to significant cost savings.

The Technicon H*1™—An Automated Hematology Analyzer for Today and Tomorrow: Complete Blood Count Parameters

1987 ◽  
Vol 87 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Pamela B. Bollinger ◽  
Benjamin Drewinko ◽  
Carrie D. Brailas ◽  
Nancy A. Smeeton ◽  
Jose M. Trujillo
Keyword(s):  
Blood Count ◽  
Complete Blood Count ◽  
Hematology Analyzer ◽  
Automated Hematology Analyzer

scholarly journals Effect of the addition of the antioxidant taurine on the complete blood count of whole blood stored at room temperature and at 4°C for up to 3 days

2017 ◽  
Vol 1 (1) ◽  
pp. 29-43
Author(s):  
Muhammad Abdul Ghaffar ◽  
Muhammad Asif Naveed ◽  
Rehma Dar
Keyword(s):  
Blood Cell ◽  
Whole Blood ◽  
Room Temperature ◽  
Blood Count ◽  
Complete Blood Count ◽  
Taurine Concentration ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
The Mean ◽  
Temperature Time

Background: The complete blood count is one of the most common routine tests. This study aimed to evaluate possible effects of the antioxidant taurine on the complete blood count of whole blood stored at room temperature and at 4°C over three days.Methods: Venous blood samples of 15 healthy males were distributed into two sets of tubes with each set of three tubes containing 50 μL of solutions with zero, 5 g/L, 10 g/L taurine. The tubes were kept at room temperature or at 4°C. Complete blood counts were performed on three successive days. The mean percentage changes [Δ = (mean value – mean baseline value) / mean baseline value x 100] were calculated and compared.Results: Complete blood count parameters exhibited different patterns of behaviour which were affected by the storage temperature, time and taurine concentration. Taurine at room temperature significantly enhanced the stability of: the platelet count over three days (Δ3 at 5 and 10 g/L taurine were 6.18, and 2.53 x 109 cells/L, respectively); the red blood cell count over three days (Δ3 at 5 and 10 g/L taurine were 2.59, and 1.39 x 1012 cells/L, respectively); mean corpuscular haemoglobin over three days (Δ3 at 5 and 10 g/L taurine were,-0.62 and -0.52 fl respectively); and red cell distribution width over two days (Δ2 at 5 and 10 g/L taurine were 1.30% and -0.1%, respectively). No additional stabilizing effects of taurine were reported for the mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, haematocrit and haemoglobin, while it negatively affected the white blood cell stability.Conclusion: Complete blood count parameters exhibited variable stability patterns in respect to temperature, time and taurine concentration.

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