A comparative evaluation of Eosin-5′-maleimide flow cytometry reveals a high diagnostic efficacy for hereditary spherocytosis

2016 ◽  
Vol 38 (5) ◽  
pp. 520-526 ◽  
Author(s):  
P. Joshi ◽  
A. Aggarwal ◽  
M. Jamwal ◽  
M. U. S. Sachdeva ◽  
D. Bansal ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Comparative Evaluation ◽  
Hereditary Spherocytosis ◽  
Diagnostic Efficacy

Comparative evaluation of fresh, fixed, and cryopreserved solid tumor cells for reliable flow cytometry of DNA and tumor associated antigen

Cytometry ◽  
1992 ◽  
Vol 13 (7) ◽  
pp. 722-729 ◽  
Author(s):  
P. A. van Dam ◽  
J. V. Watson ◽  
D. G. Lowe ◽  
T. Chard ◽  
J. H. Shepherd
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Solid Tumor ◽  
Comparative Evaluation ◽  
Tumor Associated Antigen

Intraoperative flow cytometry analysis of glioma tissue for rapid determination of tumor presence and its histopathological grade

2013 ◽  
Vol 118 (6) ◽  
pp. 1232-1238 ◽  
Author(s):  
Takahiro Shioyama ◽  
Yoshihiro Muragaki ◽  
Takashi Maruyama ◽  
Takashi Komori ◽  
Hiroshi Iseki
Keyword(s):  
Flow Cytometry ◽  
Predictive Value ◽  
Rapid Determination ◽  
Characteristic Curve ◽  
Biopsy Sample ◽  
Surgical Biopsy ◽  
Diagnostic Efficacy ◽  
Histopathological Grade ◽  
Number Of Cells

Object Intraoperative histopathological investigation plays an important role during surgery for gliomas. To facilitate the rapid characterization of resected tissue, an original technique of intraoperative flow cytometry (iFC) was established. The objective in this study was evaluation of this technique's efficacy for rapidly determining tumor presence in the surgical biopsy sample and WHO histopathological grade of the neoplasm. Methods In total, 328 separate biopsy specimens obtained during the resection of 81 intracranial gliomas were analyzed with iFC. The evaluated malignancy index (MI) was defined as the ratio of the number of cells with greater than normal DNA content to the total number of cells. The duration of iFC in all cases was approximately 10 minutes. Each sample was additionally investigated histopathologically on frozen and permanent formalin-fixed paraffin-embedded tissue sections. The latter process was used as a “gold standard” control for evaluation of the diagnostic efficacy of iFC analysis. Results The MI differed significantly between neoplastic and perilesional brain tissue (25.3% ± 22.0% vs 4.6% ± 2.6%, p < 0.01). Receiver operating characteristic curve analysis revealed a corresponding area under the curve value of 0.941. The optimal cutoff level of the MI for identification of tumor in the biopsy specimen was 6.8%, which provided 0.88 sensitivity, 0.88 specificity, 0.97 positive predictive value, 0.60 negative predictive value, and 0.88 diagnostic accuracy. Additionally, the MI showed a significant association with WHO histopathological grades of glioma (p < 0.01), but its values in Grade II, III, and IV tumors overlapped prominently and were on average 13.3% ± 11.0%, 35.0% ± 21.8%, and 46.6% ± 23.1%, respectively. Conclusions Results of this study demonstrate that iFC with the determination of the MI may be feasible for rapidly determining glioma presence in a surgical biopsy sample.

Flow Cytometry as a Diagnostic Tool for Hereditary Spherocytosis

2006 ◽  
Vol 116 (3) ◽  
pp. 186-191 ◽  
Author(s):  
Gudrun Stoya ◽  
Bernd Gruhn ◽  
Heinz Vogelsang ◽  
Eckehard Baumann ◽  
Werner Linss
Keyword(s):  
Flow Cytometry ◽  
Diagnostic Tool ◽  
Hereditary Spherocytosis

Diagnosis of hereditary spherocytosis by flow cytometry in paediatric patients. Multicentric study

2019 ◽  
Vol 493 ◽  
pp. S597
Author(s):  
B. Álvarez Flores ◽  
V. Quintero Calcaño ◽  
Á. Cervera Bravo ◽  
J. Sevilla Navarro ◽  
J. Huerta Aragonés ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Hereditary Spherocytosis ◽  
Multicentric Study ◽  
Paediatric Patients

Comparative evaluation of the parameters of spermatozoa apoptosis of young and middle-aged men by flow cytometry

2021 ◽  
Vol 172 (10) ◽  
pp. 501-504
Author(s):  
O. V. Dolgikh ◽  
◽  
D. G. Dianova ◽  
A. V. Krivtsov ◽  
I. N. Alikina ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Comparative Evaluation ◽  
Middle Aged

scholarly journals Flow cytometry test for hereditary spherocytosis

Haematologica ◽  
2012 ◽  
Vol 97 (12) ◽  
pp. e47-e47 ◽  
Author(s):  
G. Mackiewicz ◽  
F. Bailly ◽  
B. Favre ◽  
J. Guy ◽  
M. Maynadie ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Hereditary Spherocytosis

Evaluation of the Role of Flow Cytometry as a Diagnostic Tool for Hereditary Spherocytosis

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5381-5381
Author(s):  
Nalini K Pati ◽  
Mary M Sartor ◽  
Sue Wong ◽  
Adrienne Nemet ◽  
Kenneth Francis Bradstock
Keyword(s):  
Flow Cytometry ◽  
Family History ◽  
Haemolytic Anaemia ◽  
Peripheral Blood ◽  
Hereditary Spherocytosis ◽  
Positive Family History ◽  
Unknown Origin ◽  
Flow Cytometric ◽  
History Of ◽  
Normal Controls

Abstract Flow cytometric analysis of eosin-5′-maleimide-labeled red blood cells has been proposed as a method of identifying hereditary spherocytosis (HS). The flow cytometric test measures the fluorescence intensity of intact red cells labelled with the dye eosin-5-maleimide (EMA), which reacts covalently with Lys-430 on the first extra cellular loop of band 3 protein. Patients with HS have reduced fluorescence compared to other patient groups and normal controls. The aim of the present study was to assess the utility of flow cytometry in the diagnosis of hereditary spherocytosis. Fresh peripheral blood from 40 normal controls was collected in Lithium Heparin, stained with the dye EMA and analysed by flow cytometry. The normal range was established as 55–74 channel numbers. On 7 known cases of Hereditary Spherocytosis the MFI range was 35.6–44.6 channel numbers, and therefore samples falling into this range were considered to be positive for a diagnosis of HS by EMA. Equivocal results were defined when the MFI was in the range of 45–54 channel numbers. A total of 98 samples were sent for investigation or exclusion of HS. Clinical problems were grouped into the following categories: peripheral blood spherocytosis with negative Coomb’s test and no family history of HS, positive family history of HS, Neonatal hyperbilirubinaemia, haemolytic anaemia of unknown origin. Indications Coomb’s −ve, Spherocytosis, no FH (n=50) (51%) Positive family history (FH) (n=18) (17%) Neonatal hyperbilirubinemia (NNH) (n=8) (8%) Haemolytic anaemia (HA) (n=16) (17%) Results of EMA Pos Neg Equi Pos Neg Equi Pos Neg Equi Pos Neg Equi Number 19 17 13 9 7 2 2 4 2 0 14 2 Percentage 38 34 26 50 39 11 25 50 25 0 87.5 12.5 Within this cohort the group with FH of HS had the highest positive and least equivocal results. The group with suspected HS (peripheral blood spherocytosis with negative coomb’s test) resulted in positive in 38% and equivocal in 26% cases. The group with NNH had positive results in 25% of cases and the group with HA had no positive results. Equivocal results occurred in 20% of cases suggesting further investigations are required to confirm or exclude HS. The EMA dye method by flow cytometry is a useful test for diagnosing red cell membrane abnormalities due to band 3 defects, but the assay produces a substantial number of equivocal results, and has poor utility in the investigation of haemolytic anaemia of unknown origin.

Flow cytometry based on heterogeneity index score compared with urine cytology to evaluate their diagnostic efficacy in bladder tumor

Urology ◽  
1987 ◽  
Vol 29 (2) ◽  
pp. 218-222 ◽  
Author(s):  
Seido Jitsukawa ◽  
Masaaki Tachibana ◽  
Masaaki Nakazono ◽  
Hiroshi Tazaki ◽  
Joseph C. Addonizio
Keyword(s):  
Flow Cytometry ◽  
Bladder Tumor ◽  
Index Score ◽  
Urine Cytology ◽  
Diagnostic Efficacy ◽  
Heterogeneity Index
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